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Evolutionary and biogeographical approach on Australoheros angiru (Cichlidae) from lagoons in a dividing plateau between the basins of the Iguassu River and the Uruguay River, Brazil

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Abstract

Australoheros is represented by species of Cichlidae spread over different Brazilian river systems. Individuals of Australoheros angiru were collected in isolated lagoons at 950 m of altitude in a dividing plateau between the basins of the Iguassu River and the Uruguay River, located in the São Lourenço do Oeste region (Santa Catarina State), and subject to basic and molecular cytogenetic analyzes. Moreover, a review about the occurrence and collections sites of A. angiru was used to the clarification of biogeographical issues of Australoheros and other species of fish that are common between the basins of the Uruguay River and Iguassu River. The analysis by conventional staining with Giemsa found a diploid number of 48 chromosomes (18 submetacentric + 30 subtelocentric/acrocentric) for males and females, without sex chromosome differentiation. The nucleolar organizing regions identified by silver impregnation and by fluorescence in situ hybridization with 18S rDNA probes showed to be simple, located on the end of the short arm of subtelocentric chromosome pair 15. Heterochromatin was observed in the centromeric region of most of the chromosomes of the complement, in addition to coincide with the nucleolar organizing regions. The data presented in this study are the first reports on the location of sites of 5S rDNA for Australoheros, being located in interstitial position on the long arm of subtelocentric chromosome pair 11. The basic and molecular cytogenetic data presented here contribute to the better understanding of chromosomal evolution of Cichlidae, act as interesting tools for taxonomy and phylogeny of the group, in addition to help in understanding the dispersion of A. angiru between the two hydrographic basins.

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Acknowledgments

The authors are grateful to Instituto Chico Mendes de Conservação da Biodiversidade (ICMBio) for authorizing the capture of the fishes (License number: SISBIO 10522–1). The authors thank to Unioeste and to the Núcleo de Pesquisas em Limnologia, Ictiologia e Aquicultura (Nupélia) for logistical support. This study was financed by Fundação Araucária (Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Estado do Paraná), CAPES (Coordenadoria de Aperfeiçoamento de Ensino Superior) and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico).

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Authors and Affiliations

  1. Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná, Rua Universitária 2069, Cascavel, Paraná, 85819-110, Brazil

    Leonardo Marcel Paiz, Rafaela Maria Moresco & Vladimir Pavan Margarido

  2. Departamento de Biologia Celular e Genética, Universidade Estadual de Maringá, Maringá, Paraná, 87020-900, Brazil

    Lucas Baumgartner

  3. Universidade Paranaense, Campus Francisco Beltrão, Francisco Beltrão, Paraná, 85601-000, Brazil

    Fernando Rodrigo Treco

  4. Departamento de Biologia, Núcleo de Pesquisas em Limnologia, Ictiologia e Aquicultura (Nupélia), Universidade Estadual de Maringá, Maringá, Paraná, 87020-900, Brazil

    Weferson Júnio da Graça

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  1. Leonardo Marcel Paiz
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  6. Vladimir Pavan Margarido
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Correspondence to Vladimir Pavan Margarido.

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Paiz, L.M., Baumgartner, L., Moresco, R.M. et al. Evolutionary and biogeographical approach on Australoheros angiru (Cichlidae) from lagoons in a dividing plateau between the basins of the Iguassu River and the Uruguay River, Brazil. Rev Fish Biol Fisheries 24, 399–407 (2014). https://doi.org/10.1007/s11160-013-9321-y

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