Reviews in Fish Biology and Fisheries

, Volume 22, Issue 2, pp 519–526 | Cite as

Invasion, dispersion and hybridization of fish associated to river transposition: karyotypic evidence in Astyanaxbimaculatus group” (Characiformes: Characidae)

  • Wellington Adriano Moreira Peres
  • Luiz Antonio Carlos Bertollo
  • Paulo Andreas Buckup
  • Daniel Rodrigues Blanco
  • Daniel Luis Zanella Kantek
  • Orlando Moreira-Filho
Research Paper


The Astyanax species of the bimaculatus group include morphologically similar representatives that share the same color pattern, corresponding to at least 20 species. Specimens from the São Francisco River basin and from the Grande River (Upper Paraná basin) are included in this group. In the early 1960’s, the Piumhi River, an original tributary of the Grande River, was transposed to the São Francisco River basin due to the construction of the Furnas Hydroelectric Power, in the state of Minas Gerais, Brazil. In this context, we characterized representatives of the bimaculatus group from both basins and from the transposition region of the Piumhi River, using chromosome characteristics obtained by conventional analyzes and fluorescence in situ hybridization with rDNA probes. All the analyzed specimens presented 2n = 50 chromosomes and similar localization of the nucleolus organizer regions (Ag-NORs), 18S rDNA and 5S rDNA sites. However, diagnostic chromosome differences were detected concerning the number of submetacentric and acrocentric chromosomes in the karyotypes, making possible the characterization of five distinct karyotypes (Cytotypes A–E), where the specimens from the São Francisco and from the Grande River correspond to Cytotypes A and B, respectively. In addition, these two Cytotypes were sympatrically found in the Piumhi River with specimens carrying a clear intermediate karyotype (Cytotype C), as well as with others harboring different karyotypes (Cytotypes D and E). Our data indicate that Cytotypes A and B probably diverged from each other during the prior geographic isolation. However, despite their chromosomal differentiations, they still maintain a close relationship which allows interbreeding and the formation of a secondary hybrid zone, due to the breakdown of the geographic isolation.


Fish Chromosome polymorphism Isolation breakdown Hybrid zone 



The authors thank to Luis Henrique da Silva and Pedro Luis Gallo for the help in the fish collections; to the Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis (IBAMA—License number 10538-1) and Instituto Estadual de Florestas (IEF/MG) for the collection’ authorizations. This study was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo—FAPESP (Proc. 05/58630-3) and by Conselho Nacional de Desenvolvimento Científico e Tenoclógico—CNPq, through research projects granted to OMF, LACB and PAB.


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Wellington Adriano Moreira Peres
    • 1
  • Luiz Antonio Carlos Bertollo
    • 4
  • Paulo Andreas Buckup
    • 2
  • Daniel Rodrigues Blanco
    • 4
  • Daniel Luis Zanella Kantek
    • 3
  • Orlando Moreira-Filho
    • 4
  1. 1.Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis-IBAMACanaranaBrazil
  2. 2.Departamento de Vertebrados, Museu NacionalUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil
  3. 3.Instituto Chico Mendes de Conservação da Biodiversidade—ICMBioEstação Ecológica de TaiamãCáceresBrazil
  4. 4.Departamento de Genética e EvoluçãoUniversidade Federal de São CarlosSão CarlosBrazil

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