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Lack of reproductive isolation between the Western and Eastern phylogroups of the tench

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Abstract

The Eurasian range of the tench distribution is subdivided into deeply divergent Western and Eastern phylogroups evidenced by nuclear and mitochondrial DNA sequence markers. A broad zone of overlap exists in central and western Europe, suggesting post-glacial contact with limited hybridisation. We conducted a population genetic test of this indication that the two phylogroups may represent distinct species. We analysed variation at introns of nuclear genes, microsatellites, allozymes and mitochondrial DNA in populations from two postglacial lakes within the contact zone in Germany. The test is based on the expectation that in the presence of strong barriers to reproduction, a hybrid population will show genome-wide associations among alleles and genotypes from each phylogroup even after hundreds of generations of interbreeding. In contrast to this expectation, no consistent significant deviations from linkage and Hardy–Weinberg equilibria were found. Samples from both lakes did show significant disequilibria but they were limited to individual loci and were not concordant between populations, and were not robust to the method used. The single consistent association can be attributed to physical linkage between two microsatellite loci. Thus, results of our study support the hypothesis of free interbreeding between the two phylogroups of tench. Therefore, although the phylogroups may be considered as separate phylogenetic species, the present data suggest that they are a single species under the biological species concept.

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Acknowledgments

The authors thank Petra Kersten for laboratory assistance and Silvia Marková and Petr Ráb for advice. The work was supported by the Ministry of Education, Youth and Sports of the Czech Republic (LC06073, MSM6007665809), and by the Academy of Sciences of the Czech Republic (IRP IAPG AV0Z50450515, IGA UZFG/05/22 and IGA UZFG/08/13).

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Authors and Affiliations

  1. Department of Vertebrate Evolutionary Biology and Genetics, Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Rumburská 89, 277 21, Liběchov, Czech Republic

    Zdeněk Lajbner & Petr Kotlík

  2. Department of Zoology, Faculty of Science, Charles University, 128 44, Prague, Czech Republic

    Zdeněk Lajbner

  3. Department of Aquaculture and Ecophysiology, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Mueggelseedamm 310, P.O. Box 850119, 12561, Berlin, Germany

    Klaus Kohlmann

  4. University of South Bohemia, České Budějovice, Research Institute of Fish Culture and Hydrobiology at Vodňany, Zátiší 728/II, 389 25, Vodňany, Czech Republic

    Otomar Linhart

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  1. Zdeněk Lajbner
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  2. Klaus Kohlmann
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  3. Otomar Linhart
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  4. Petr Kotlík
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Correspondence to Zdeněk Lajbner.

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Lajbner, Z., Kohlmann, K., Linhart, O. et al. Lack of reproductive isolation between the Western and Eastern phylogroups of the tench. Rev Fish Biol Fisheries 20, 289–300 (2010). https://doi.org/10.1007/s11160-009-9137-y

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