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Image based kinetic determination of iron(III) in blood samples using a CCD camera

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Abstract

Digital image-based calibration was applied for the determination of iron(III), Fe3+, as a catalyst in the oxidation of indigo carmine (IC) using bromate ion (BrO3 −). During the reaction, a colorless product was produced and there was an induction period (IP) before the color of the solution faded. The length of IP depended on the concentration of Fe3+. The color fading of reaction was recorded as a change of red, blue and green colors (%RBG). Kinetic profiles similar to what has been reported in a previous spectrophotometric study were obtained. Fe3+ was determined in the concentration range of 6–16 ppm at pH = 2 and 25 °C. The detection limit of Fe3+ was found to be 1.994 ppm, which is better in comparison with the previous work. Optimum concentrations of IC and BrO3 − were obtained as 5.5 × 10−5 and 3 × 10−3 M, respectively, using a one-at-a-time optimization method. The optimum pH value was 2. The interference effect of various cations and anions on the determination of iron is reported. In addition, the application of the method to real samples of human serum was performed. The obtained results from real samples were in accordance with the obtained results using standard methods. The analysis of images (movies) recorded from an evolutionary chemical systems seems to open new, simple and low-cost analytical opportunities.

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References

  1. Wongwilai W, Lapanantnoppakhun S, Grudpan S, Grudpan K (2010) Webcam camera as a detector for a simple lab-on-chip time based approach. Talanta 81(3):1137–1141

    Article  CAS  Google Scholar 

  2. Maleki N, Safavi A, Sedaghatpour F (2004) Single-step calibration, prediction and real samples data acquisition for artificial neural network using a CCD camera. Talanta 64(4):830–835

    Article  CAS  Google Scholar 

  3. Gaiao EdN, Martins VL, Lyra WdS, Almeida LFd, Silva ECd, Araújo MCU (2006) Digital image-based titrations. Anal Chim Acta 570(2):283–290

    Article  CAS  Google Scholar 

  4. Miyamoto T, Kuramitsu Y, Ookuma A, Trevanich S, Honjoh K, Hatano S (1998) Rapid detection and counting of viable bacteria in vegetables and environmental water using a photon-counting TV camera. J Food Protect 61:1312–1316

    CAS  Google Scholar 

  5. Akiyama M, Sugatani J, Suzuki Y, Miwa M (1994) Determination of platelet-activating factor acetylhydrolase activity by blotting, [beta]-radioluminescence, and ultrahigh-sensitivity television camera detection. Anal Biochem 218(2):295–299

    Article  CAS  Google Scholar 

  6. Lopez-Molinero A, Liñan D, Sipiera D, Falcon R (2010) Chemometric interpretation of digital image colorimetry. Application for titanium determination in plastics. Microchem J 96(2):380–385

    Article  CAS  Google Scholar 

  7. Ray E, Bunton P, Pojman JA (2007) Determination of the diffusion coefficient between corn syrup and distilled water using a digital camera. Am J Phys 75(10):903–906

    Article  CAS  Google Scholar 

  8. Momeni N, Ramanathan K, Larsson PO, Danielsson B, Bengmark S, Khayyami M (1999) CCD-camera based capillary chemiluminescent detection of retinol binding protein. Anal Chim Acta 387(1):21–27

    Article  CAS  Google Scholar 

  9. Cho I-H, Paek E-H, Kim Y-K, Kim J-H, Paek S-H (2009) Chemiluminometric enzyme-linked immunosorbent assays (ELISA)-on-a-chip biosensor based on cross-flow chromatography. Anal Chim Acta 632(2):247–255

    Article  CAS  Google Scholar 

  10. Safavi A, Maleki N, Rostamzadeh A, Maesum S (2007) CCD camera full range pH sensor array. Talanta 71(1):498–501

    Article  CAS  Google Scholar 

  11. Duponchel L, Milanfar P, Ruckebusch C, Huvenne J-P (2008) Super-resolution and Raman chemical imaging: from multiple low resolution images to a high resolution image. Anal Chim Acta 607(2):168–175

    Article  CAS  Google Scholar 

  12. Borman S (1996) Array detectors are transforming optical spectroscopy ACS. Available via ACS. http://pubs.acs.org/cen/hotarticles/cenear/960318/array.html

  13. Lavigne JJ, Savoy S, Clevenger MB, Ritchie JE, McDoniel B, Yoo S-J, Anslyn EV, McDevitt JT, Shear JB, Neikirk D (1998) Solution-based analysis of multiple analytes by a sensor array: toward the development of an “electronic tongue”. J Am Chem Soc 120(25):6429–6430

    Article  CAS  Google Scholar 

  14. Stoyanova A (2006) Spectrophotometric determination of iron(III) based on its catalytic effect on the oxidation of diphenylamine with hydrogen peroxide in the presence of cetylpyridinium chloride. J Univ Chem Technol Metall 41(2):205–210

    CAS  Google Scholar 

  15. Massoumi A, Tavallali H (1998) Kinetic spectrophotometric determination of vanadium by catalytic effect on the indigo carmine–bromate reaction. Anal Lett 31(1):193–206

    Article  CAS  Google Scholar 

  16. Tavallali H, Massoumi A (1998) Simultaneous kinetic spectrophotometric determination of vanadium(V) and iron(III). Talanta 47(2):479–485

    Article  CAS  Google Scholar 

  17. Miller JC, Miller JN (1993) Statistics for analytical chemistry. Ellis Horwood, New York

    Google Scholar 

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Acknowledgments

The authors are grateful to Shiraz Payam Noor University for financial support of this study and Dr Charsoughi at Department of Physics in Institute for Advanced Studies in Basic Sciences (IASBS) for his kind collaboration in using of the software.

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Authors and Affiliations

  1. Departments of Chemistry, Institute for Advanced Studies in Basic Sciences (IASBS), GavaZang, 45137-66731, Zanjan, Iran

    M. Kompany-Zareh & M. Khoshkam

  2. Department of Chemistry, Payame Noor University, 71365-944, Shiraz, Iran

    H. Tavallali, N. Shakernasab & E. Shamsdin

Authors
  1. M. Kompany-Zareh
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  2. H. Tavallali
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  3. N. Shakernasab
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  4. M. Khoshkam
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  5. E. Shamsdin
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Correspondence to M. Kompany-Zareh.

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Kompany-Zareh, M., Tavallali, H., Shakernasab, N. et al. Image based kinetic determination of iron(III) in blood samples using a CCD camera. Reac Kinet Mech Cat 107, 49–61 (2012). https://doi.org/10.1007/s11144-012-0453-1

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  • DOI: https://doi.org/10.1007/s11144-012-0453-1

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