Abstract
Polyphenols (PP) found in brown algae are known for their wide range of biological activities including noteworthy antitumor properties. This article presents a method for obtaining an active polyphenolic extract from the Arctic alga Fucus vesiculosus with 98% purity and radical scavenging activity equivalent to 862 mg of ascorbic acid per gram of extract. Immunostimulant effects of polyphenols were assessed in vitro using venous blood from two groups of people: healthy people (HP) and people with chronic undifferentiated lymphocytic leukemia (LP). Polyphenols activated the surface properties of immunocompetent cells. Specifically, polyphenols dose-dependently increased the percentage of cells’ spreading and adhesion by 2–3 times. Additionally, polyphenols increased the number of activated lymphocytes in the LP blood to levels characteristic of HP. Given their natural origin, high activity, non-toxicity, and straightforward production process, these studied polyphenols exhibit immense potential for use as new pharmaceuticals or as active components with immunostimulatory effects.
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This work was supported by the Ministry of Science and Higher Education, project No. FSRU-2023-004.
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Conceptualization: K.B., L.D.; Methodology: L.D., A.S.; Formal analysis and investigation: L.D., A.S., A.P.; Writing - original draft preparation: A.P.; Writing - review and editing: L.D., K.B.; Supervision: K.B.
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All experiments were conducted in accordance with the World Medical Association Declaration of Helsinki – Ethical principles for medical research involving human subjects (2013). The studies were approved by the Biomedical Ethics Commission at the N.P. Laverov FCIAR of the Ural Branch of the Russian Academy of Sciences (protocol No. 8, 30 March, 2022). Informed consent was obtained from all individual participants (volunteers) included in the study.
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Bogolitsyn, K., Dobrodeeva, L., Samodova, A. et al. In vitro Immunostimulant Activity of the Polyphenolic Extract from the Arctic Brown Algae Fucus vesiculosus. Plant Foods Hum Nutr (2024). https://doi.org/10.1007/s11130-024-01174-x
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DOI: https://doi.org/10.1007/s11130-024-01174-x