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Anti-α-glucosidase, Anti-proliferative and Anti-enterovirus 71 Activity of Secondary Metabolites Identified from Grifola Frondosa

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Abstract

Grifola frondosa, an edible and medicinal resource, is widely used as functional foods worldwide. To explore bioactive compounds against α-glucosidase, human tumor cells and enterovirus 71 (EV71), eight compounds were isolated from G. frondosa by chromatographic column. Among the isolated compounds, heptadecanoic acid, uridine and adenosine exhibited potent inhibition activity against α-glucosidase, ergosterols and ergosterol-5,8-peroxide showed anti-proliferative activity on tumor cells, while ergosterol and methyl linoleate displayed inhibition against the replication of EV71. Also, to our knowledge, this is the first study to report that fatty acids isolated from G. frondosa show potent inhibition against α-glucosidase and EV71. Further molecular docking results revealed that the active compounds in G. frondosa form hydrogen bonding, hydrophobic interactive and π-stacking with the active sites on the surface of α-glucosidase, CASP3 and VP1 proteins, thus promoting the active compounds to combine with the target protein to form a stable complex, thus playing an antagonistic role. Our results could provide a new active compound and mode of action for G. frondosa to treat diabetes, cancer and EV71-infected patients.

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Data Availability

All data generated or analyzed during this study are included in this published article (and its supplementary information files).

Abbreviations

EV71:

enterovirus 71

HFMD:

hand-foot-and-mouth disease

FBS:

fetal bovine serum

DMEM:

dulbecco’s modified eagle’s medium

OCFA:

odd-chain fatty acids

TI:

therapeutic index

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Funding

This work was supported by Major Special Project of Fujian Province “Research and application of key technologies for innovation and industrialized utilization of Juncao” (2021NZ029009), Fujian Provincial Department of Science and Technology Regional Development Project (2020N3002), Foreign Cooperation Project of Fujian Provincial Department of Science and Technology (2021I0008) and Interdisciplinary integration to promote the high-quality development of Juncao science and Industry (XKJC-712021030).

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Authors and Affiliations

  1. College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China

    Junqiang He, Wenyu Xiong, Lina Zhao, Bin Liu & Ying Huang

  2. National Engineering Research Center of JUNCAO Technology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Lina Zhao & Bin Liu

Authors
  1. Junqiang He
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  2. Wenyu Xiong
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  3. Lina Zhao
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  4. Bin Liu
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Contributions

Conceptualization, Junqiang He and Ying Huang; Methodology, Junqiang He and Wenyu Xiong; Writing – Original Draft, Junqiang He; Writing Review and Editing, Junqiang He and Ying Huang; Funding Acquisition, Bin Liu and Lina Zhao; Supervision, Bin Liu and Ying Huang.

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Correspondence to Bin Liu or Ying Huang.

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He, J., Xiong, W., Zhao, L. et al. Anti-α-glucosidase, Anti-proliferative and Anti-enterovirus 71 Activity of Secondary Metabolites Identified from Grifola Frondosa. Plant Foods Hum Nutr 78, 783–789 (2023). https://doi.org/10.1007/s11130-023-01106-1

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