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Nutritional Composition and Pharmacological Activity of Musa balbisiana Colla Seed: An Insight into Phytochemical and Cellular Bioenergetic Profiling

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Abstract

Musa balbisiana Colla belongs to the family Musaceae which is well-known for its nutritional and pharmacological properties. Here, we have analysed the phytochemical content and evaluated the nutritional, antioxidant, anti-glycation, α-amylase, and α-glucosidase inhibition potential. Moreover, for the first time, we have studied the bioenergetic profiles of the bioactive fractions of M. balbisiana seeds extract against oxidative stress-related mitochondrial and cellular dysfunction using XFe24 extracellular flux analyzer. M. balbisiana seeds have high nutritional values with significant levels of carbohydrates, starch, protein, and minerals (Ca, Na, Mg, Cu, Fe, and Zn). Bioactivity-guided fractionation of the methanolic extract of M. balbisiana seeds revealed that the ethyl acetate fraction (EAF) showed the highest antioxidant, anti-glycation, and phytochemical content as compared to other fractions. Moreover, the EAF showed a lower α-amylase inhibition and a higher α-glucosidase inhibitory activity. Most importantly, our GC-MS analyses of EAF revealed the presence of unique and previously unreported 14 phytochemical compounds. A strong correlation between the biological activities and total phenolic/tannin content was observed. In addition, the bioactive fraction of M. balbisiana seeds (EAF) improved the bioenergetic profiles of free fatty acid-induced oxidative stress with a concomitant increase in ATP production, and respiratory and glycolytic capacity. Altogether, our findings suggest that M. balbisiana seeds can be used as a natural supplement to boost antioxidant levels and combat oxidative stress and non-enzymatic glycation.

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Data Availability

All data generated or analysed during this study are included in this published article [and its supplementary information files].

Abbreviations

ABTS:

(2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)

AG:

Aminoguanidine

AGEs:

Advanced glycation end products

BSA:

Bovine serum albumin

CE:

Catechin equivalent

DPPH:

(1,1-diphenyl-2-picrylhydrazyl)

ECAR:

Extracellular acidification rate

EAF:

Ethyl acetate fraction

FFA:

Free fatty acid

FRAP:

Ferric reducing antioxidant power

GAE:

Gallic acid equivalent

GC:

Glycolytic capacity

GR:

Glycolytic reserve

ME:

Methanolic extract

MR:

Maximum respiration

nBF:

n-butanol fraction

OCR:

Oxygen consumption rate

ROS:

Reactive oxygen species

SRC:

Spare respiratory capacity

TPC:

Total phenolic content

TFC:

Total flavonoid content

TTC:

Total tannin content

QE:

Quercetin equivalent

WF:

Water fraction

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Acknowledgements

Nonibala Gurumayum would like to acknowledge the University Grant Commission (UGC), Government of India, for manpower support in the form of a Junior Research Fellowship. The authors are grateful to the Sophisticated Analytical Instrumentation Centre (SAIC), Institute of Advanced Study in Science and Technology (IASST), Guwahati (under the Department of Science & Technology, Government of India) for providing the necessary equipment.

Funding

This work was supported by IASST in-house core-funded research project no. IASST/R&D/LSD/CP-01/2021/01/2021-22/5336–5345.

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Authors and Affiliations

  1. Life Sciences Division, Institute of Advanced Study in Science and Technology (IASST), Guwahati, 781035, Assam, India

    Nonibala Gurumayum, Partha Pratim Sarma, Puspanjali Khound, Uttam Kumar Jana & Rajlakshmi Devi

  2. Department of Zoology, Gauhati University, Guwahati, 781014, India

    Nonibala Gurumayum, Puspanjali Khound & Rajlakshmi Devi

  3. Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, Kolkata, 700064, India

    Uttam Kumar Jana

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  1. Nonibala Gurumayum
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Contributions

Nonibala Gurumayum and Rajlakshmi Devi - conceptualization & study design; Nonibala Gurumayum - Methodology, investigation, analysis & writing original draft; Partha Pratim Sarma and Puspanjali Khound - investigation; Uttam Kumar Jana and Rajlakshmi Devi - writing (Manuscript review & editing); Rajlakshmi Devi - supervision.

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Correspondence to Rajlakshmi Devi.

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Gurumayum, N., Sarma, P.P., Khound, P. et al. Nutritional Composition and Pharmacological Activity of Musa balbisiana Colla Seed: An Insight into Phytochemical and Cellular Bioenergetic Profiling. Plant Foods Hum Nutr 78, 520–525 (2023). https://doi.org/10.1007/s11130-023-01080-8

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