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Heated Leaf Extract of Coriandrum sativum L. Protects Nigral Dopaminergic Degeneration in Rats


Coriandrum sativum L. (coriander), which is an annual herb of the Apiaceae family, has been traditionally used as a remedy. Here we tested whether heated extract of coriander leaf protects nigral dopaminergic neurodegeneration after exposure to 6-hydroxydopamine (6-OHDA). After injection of 6-OHDA into the rat substantia nigra pars compacta (SNpc), dopaminergic degeneration, which was determined by tyrosine hydroxylase immunostaining, was rescued by co-injection of CaEDTA, an extracellular Zn2+ chelator, suggesting that extracellular Zn2+ influx is involved in neurodegeneration. Both intracellular Zn2+ dysregulation determined by ZnAF-2 fluorescence and dopaminergic degeneration in the SNpc induced by 6-OHDA were rescued by co-injection of 0.25% coriander extract, which also reduced reactive oxygen species (ROS) production in the SNpc determined by aminophenyl fluorescein fluorescence. The present study suggests that coriander leaf extract protects nigral dopaminergic neurodegeneration induced by intracellular Zn2+ dysregulation. It is likely that the nutraceutical property of coriander leaf extract contributes to the protection via reducing ROS production.

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  1. Kouli A, Torsney KM, Kuan WL, Thomas BS, Greenland JC (2018) In: Parkinson’s Disease: Pathogenesis and Clinical Aspects [Internet]. Brisbane (AU): Codon Publications, pp 3–26.

  2. Rodriguez-Pallares J, Parga JA, Joglar B, Guerra MJ, Labandeira-Garcia JL (2009) The mitochondrial ATP-sensitive potassium channel blocker 5-hydroxydecanoate inhibits toxicity of 6-hydroxydopamine on dopaminergic neurons. Neurotox Res 15:82–95.

  3. Tamano H, Nishio R, Morioka H, Takeda A (2019) Extracellular Zn2+ influx into nigral dopaminergic neurons plays a key role for pathogenesis of 6-hydroxydopamine-induced Parkinson’s disease in rats. Mol Neurobiol 56:435–443.

    CAS  Article  PubMed  Google Scholar 

  4. Nishio R, Morioka H, Takeuchi A, Saeki N, Furuhata R, Katahira M, Chinenn T, Tamura H, Tamano H, Takeda A (2022) Intracellular hydrogen peroxide produced by 6-hydroxydopamine is a trigger for nigral dopaminergic degeneration of rats via rapid influx of extracellular Zn2+. Neurotoxicology 89:1–8.

    CAS  Article  PubMed  Google Scholar 

  5. Laribi B, Kouki K, M'Hamdi M, Bettaieb T (2015) Coriander (Coriandrum sativum L.) and its bioactive constituents. Fitoterapia. 2015 103:9–26.

  6. Gazwi HSS, Mahmoud ME, Toson EMA (2022) Analysis of the phytochemicals of Coriandrum sativum and Cichorium intybus aqueous extracts and their biological effects on broiler chickens. Sci Rep 12:6399.

  7. Verma T, Sinha M, Bansal N, Yadav SR, Shah K, Chauhan NS (2021) Plants used as antihypertensive. Nat Prod Bioprospect 11:155–184.

  8. Gray AM, Flatt PR (1999) Insulin-releasing and insulin-like activity of the traditional anti-diabetic plant Coriandrum sativum (coriander). Br J Nutr 81:203–209.

  9. Cioanca O, Hritcu L, Mihasan M, Hancianu M (2013) Cognitive-enhancing and antioxidant activities of inhaled coriander volatile oil in amyloid β (1–42) rat model of Alzheimer’s disease. Physiol Behav 120:193–202.

  10. Nishio R, Tamano H, Morioka H, Takeuchi A, Takeda A (2019) Intake of heated leaf extract of Coriandrum sativum contributes to resistance to oxidative stress via decreases in heavy metal concentrations in the kidney. Plant Foods Hum Nutr 74:204–209.

    CAS  Article  PubMed  Google Scholar 

  11. Kim EY, Koh JY, Kim YH, Sohn S, Joe E, Gwag BJ (1999) Zn2+ entry produces oxidative neuronal necrosis in cortical cell cultures. Eur J Neurosci 11:327–334.

    CAS  Article  PubMed  Google Scholar 

  12. Kim YH, Eom JW, Koh JY (2020) Mechanism of zinc excitotoxicity: a focus on AMPK. Front Neurosci 14:577958.

  13. Sensi SL, Paoletti P, Bush AI, Sekler I (2009) Zinc in the physiology and pathology of the CNS. Nat Rev Neurosci 10:780–791.

    CAS  Article  PubMed  Google Scholar 

  14. Medvedeva YV, Ji SG, Yin HZ, Weiss JH (2017) Differential vulnerability of CA1 versus CA3 pyramidal neurons after ischemia: possible relationship to sources of Zn2+ accumulation and its entry into and prolonged effects on mitochondria. J Neurosci 37:726–737.

  15. Ji SG, Medvedeva YV, Wang HL, Yin HZ, Weiss JH (2019) Mitochondrial Zn 2+ accumulation: a potential trigger of hippocampal ischemic injury. Neuroscientist 25:126–138.

  16. Ji SG, Medvedeva YV, Weiss JH (2020) Zn 2+ entry through the mitochondrial calcium uniporter is a critical contributor to mitochondrial dysfunction and neurodegeneration. Exp Neurol 325:113161.

    CAS  Article  PubMed  Google Scholar 

  17. Noh KM, Koh JY (2000) Induction and activation by zinc of NADPH oxidase in cultured cortical neurons and astrocytes. J Neurosci 20:RC111.

  18. Kabuto H, Yamanushi TT (2011) Effects of zingerone [4-(4-hydroxy-3-methoxyphenyl)-2-butanone] and eugenol [2-methoxy-4-(2-propenyl)phenol] on the pathological progress in the 6-hydroxydopamine-induced Parkinson’s disease mouse model. Neurochem Res 36:2244–2249.

    CAS  Article  PubMed  Google Scholar 

  19. Bigham M, Mohammadipour A, Hosseini M, Malvandi AM, Ebrahimzadeh-Bideskan A (2021) Neuroprotective effects of garlic extract on dopaminergic neurons of substantia nigra in a rat model of Parkinson’s disease: motor and non-motor outcomes. Metab Brain Dis 36:927–937.

    CAS  Article  PubMed  Google Scholar 

  20. Koppula S, Alluri R, Kopalli SR (2021) Coriandrum sativum attenuates microglia mediated neuroinflammation and MPTP-induced behavioral and oxidative changes in Parkinson's disease mouse model. EXCLI J 20:835–850.

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Conceptualization; Atsushi Takeda: Data curation; Haruna Tamano: Formal analysis; Nana Saeki, Azusa Takeuchi, Misa Katahira, Ryusuke Nishio, Haruna Tamura: Methodology; Nana Saeki, Atsushi Takeda: Project administration; Atsushi Takeda: Resources; Atsushi Takeda: Software; Nana Saeki: Supervision; Haruna Tamano: Validation; Atsushi Takeda: Roles/Writing—original draft; Atsushi Takeda: Writing—review & editing; Atsushi Takeda.

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Correspondence to Atsushi Takeda.

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The Ethics Committee for Experimental Animals has permitted the present study in the University of Shizuoka.

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Saeki, N., Tamano, H., Takeuchi, A. et al. Heated Leaf Extract of Coriandrum sativum L. Protects Nigral Dopaminergic Degeneration in Rats. Plant Foods Hum Nutr 77, 455–459 (2022).

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  • Parkinson's disease
  • 6-hydroxydopamine
  • Dopaminergic neurodegeneration
  • Reactive oxygen species
  • Zn2+
  • Coriandrum sativum L.