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Anti-Diabetic Potential of Chlorella Pyrenoidosa-Based Mixture and its Regulation of Gut Microbiota

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Abstract

The aim of the present study was to investigate the anti-diabetic effect of CGSGCG and its beneficial effects on gut microbiota in type 2 diabetes (T2D) mice induced by streptozotocin and high sucrose and high fat diet. The results showed that treatment with CGSGCG reduced fasting blood glucose, improved oral glucose tolerance test, protected the liver from injury, and reduced inflammation in T2D mice. The contents of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid in CGSGCG group were 2.49-, 1.74-, 3.31-, 1.93-, 1.36- and 1.30-fold than that of the model group. Moreover, administration of CGSGCG up-regulated the expression of INSR/IRS-1/PI3K/AKT/GLUT4 and mTOR but down-regulated the P38MAPK expression. Furthermore, the abundance of beneficial bacteria such as Verrucomicrobia, Proteobacteria, Osillibacter, Dubosiella and Lactococcus in intestinal tract increased, indicating that CGSCGG regulated and improved the bacterial community structure of T2D mice, which were closely related to glycometabolism.

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Data Availability

All data generated or analyzed during this study are included in this published article (and its supplementary information files).

Code Availability

Not applicable.

Abbreviations

T2D:

Type 2 diabetes

SOD:

Superoxide dismutase

STZ:

Streptozotocin

HSHF:

High sucrose and high fat

GFP:

Polysaccharides from Grifola frondosa

SCFAs:

Short chain fatty acids

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Acknowledgments

This work was financially supported by Xiamen Marine Science and Technology Achievement Transformation and Industrialization Demonstration Project (18CYY026HJ13) and Major Science and Technology Project of Fujian Province (2020NZ012010).

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Author notes

  1. Wenyu Xiong and Jie Chen contributed equally to this work.

Authors and Affiliations

  1. Engineering Research Center of Fujian and Taiwan Special Marine Food Processing and Nutrition, Ministry of Education, Fuzhou, 350002, China

    Wenyu Xiong, Jie Chen, Junqiang He, Meifang Xiao, Xiaoyu He, Bin Liu & Feng Zeng

  2. College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Wenyu Xiong, Jie Chen, Junqiang He, Meifang Xiao, Bin Liu & Feng Zeng

  3. Fuzhou Ocean Research Institute Marine Food Research and Development Center, Fuzhou, 350002, China

    Xiaoyu He, Bin Liu & Feng Zeng

  4. Xiamen 139 Biotechnology Co., Ltd., Xiamen, 361000, China

    Bin Liu

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Contributions

Wenyu Xiong and Jie Chen conducted the study, drafted the first manuscript; Junqiang He analyzed the data, read and approved the manuscript; Meifang Xiao and Xiaoyu He contributed to data interpretation and supervised the study; Bin Liu conceived and designed the study; Feng Zeng critically reviewed the manuscript. All authors approved the final manuscript for submission.

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Correspondence to Bin Liu or Feng Zeng.

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Xiong, W., Chen, J., He, J. et al. Anti-Diabetic Potential of Chlorella Pyrenoidosa-Based Mixture and its Regulation of Gut Microbiota. Plant Foods Hum Nutr 77, 292–298 (2022). https://doi.org/10.1007/s11130-022-00968-1

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  • DOI: https://doi.org/10.1007/s11130-022-00968-1

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