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A Novel Auraptene-Enriched Citrus Peels-Based Blend with Enhanced Antioxidant Activity


The aim of this study was to establish a convenient process to get auraptene-enriched blends from Citrus limon L. Osbeck (Rutaceae) peels powder with functional food and nutraceutical potential and antioxidant properties. The process consisted in the selective adsorption of auraptene by bentonite followed by desorption and evaporation of solvents to get the final product. HPLC analyses revealed an adsorption > 99% from lemon fruit peel powder and a 13.7-fold enrichment of the obtained composition respect to the parent food-derived sample. The antioxidant and radical scavenger activities of the Citrus peels based preparation were assayed by the DPPH and ABTS tests and reveal deffects higher than ascorbic acid and Trolox® used as the references. To the best of our knowledge, the findings described herein is the first example reported in the literature of solid phase adsorption experiments carried out on auraptene as a component of edible fruits.

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Data Availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.


  1. 1.

    Genovese S, Fiorito S, Locatelli M, Carlucci G, Epifano F (2014) Analysis of biologically active ferulic acid derivatives in Citrus fruits. Plant Foods Hum Nutr 69:255–260.

    CAS  Article  PubMed  Google Scholar 

  2. 2.

    Genovese S, Epifano F, Palumbo L, Pulito G, Bastianini M, Cardellini F, Spogli R, Fiorito S (2020) Solid phase adsorption of emodin on hydrotalcites and inorganic oxide: a preliminary study. J Pharm Biomed Anal 187:113348.

    Article  PubMed  Google Scholar 

  3. 3.

    Gulcin I (2020) Antioxidants and antioxidant method: an updated review. Arch Toxicol 94:651–715.

    CAS  Article  PubMed  Google Scholar 

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This study was supported by the University “G. D’Annunzio” of Chieti-Pescara (Fondi FAR 2020).

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Correspondence to Francesco Epifano.

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Fiorito, S., Epifano, F., Palumbo, L. et al. A Novel Auraptene-Enriched Citrus Peels-Based Blend with Enhanced Antioxidant Activity. Plant Foods Hum Nutr 76, 397–398 (2021).

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  • Auraptene
  • Bentonite
  • Citrus limon
  • Oxyprenylated coumarins
  • Solid sorbents