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Supplementation with Phaseolus vulgaris Leaves Improves Metabolic Alterations Induced by High-Fat/Fructose Diet in Rats Under Time-Restricted Feeding


Time-restricted feeding and food enriched in polyphenols are strategies to prevent or reduce metabolic disorders. Bean leaves (Phaseolus vulgaris L.) are a recognized source of polyphenolic compounds, whose effects on metabolic pathways are not well studied. We evaluated the combined effects of dietary supplementation with Phaseolus vulgaris leaves (10% w/w) (BL) and a 7-h daytime-restricted feeding protocol (RF) under a hypercaloric diet (high fat + high fructose) (HFFD) on the metabolic parameters related to glucose and lipid handling. Adult male Wistar rats were treated for 8 weeks with standard and HFFD diets with or without BL. The results showed that RF improved metabolic alterations induced by HFFD (e.g., hepatic steatosis, increased triacylglycerols, and serum lipoproteins). Supplementation with BL significantly enhanced this effect and downregulated the mRNA expression of carbohydrate and lipid metabolism genes in the liver. These results indicate that dietary supplementation with BL enhances the benefits elicited by RF.

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Acetyl-CoA carboxylase


Bean leaves


Fatty acid synthase




Glucose-6-phosphate dehydrogenase




High-density lipoprotein


High fat + high fructose diet


Homeostatic Model Assessment for Insulin Resistance


Low-density lipoprotein


Phosphoenolpyruvate carboxykinase


Pyruvate kinase


Restricted feeding


Stearoyl-CoA desaturase-1


Standard diet




Very-low-density lipoprotein


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We thank Jéssica González Norris for assistance with the English version of this manuscript, and Dr. Christian Molina Aguilar, Dr. Aracely Anaya-Loyola, Nut. Sonia Hernández-Nájera, Nut. Fernando López-Barrera, Lilia Ruíz-Garduño, Biol. Josué López-Martínez, Ing. Nydia Hernández-Ríos, and Nut. Adriana Becerril-Campos for their technical support.

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Correspondence to D. Luna-Moreno.

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Ramírez-Venegas, G., De Ita-Pérez, D., Díaz-Muñoz, M. et al. Supplementation with Phaseolus vulgaris Leaves Improves Metabolic Alterations Induced by High-Fat/Fructose Diet in Rats Under Time-Restricted Feeding. Plant Foods Hum Nutr 76, 297–303 (2021).

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  • Phaseolus vulgaris
  • Bean leaves
  • Restricted feeding
  • Metabolic alterations
  • High-fat/fructose diet