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Physiological Characteristics, Phytase Activity, and Mineral Bioavailability of a Low-Phytate Soybean Line during Germination

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Abstract

Phytate is a storage form of phosphorus (P) in seeds and, when degraded, plays a vital role in seed germination. Low phytate content in grain seeds inhibits germination and seedling growth. Here, Low-phytate (LP) and normal-phytate (NP) soybean lines were grown in vermiculite to determine whether germination was affected by low phytate content. Growth, phytase activity, mineral concentration, and mineral extractability from the seedlings of NP and LP soybean lines were evaluated. Seedling growth did not differ significantly between NP and LP lines. Phytase and specific phytase activities at 11 days to 17 days after sowing were 1.3 to 2.6 folds greater in the NP line than in the LP line. The LP line hydrolyzed all the phytate 2 days earlier than the NP line. The concentration and extractability of minerals changed over time during germination, and the LP line had higher molar ratios of phytic acid to Ca, Mg, and K than the NP line. These results suggest that germination and growth of the seedlings are not affected by low phytate levels in seeds. We suggest that the LP line benefits from higher bioavailability of P and macro-minerals unlike the NP line.

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Acknowledgements

This study was funded by Japan Society for the Promotion of Science KAKENHI: Grant Number 18 K05948 to Saneoka H. Financial support was received also from the China Scholarship Council to Mr. Qin Dong. The authors would like to thank Mrs. Hosokawa T., Futamura Y., Natsume Y., Masaoka K., Okamoto T., Wakabayashi K, and Ms. Fukuda Y., Tasukawa E., Kakizawa H., Kumagai M., Hara Y., Ogawa M. for contribution to breeding low phytate lines.

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  1. Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima, 739-8528, Japan

    Qin Dong & Hirofumi Saneoka

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  1. Qin Dong
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  2. Hirofumi Saneoka
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Correspondence to Hirofumi Saneoka.

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Dong, Q., Saneoka, H. Physiological Characteristics, Phytase Activity, and Mineral Bioavailability of a Low-Phytate Soybean Line during Germination. Plant Foods Hum Nutr 75, 383–389 (2020). https://doi.org/10.1007/s11130-020-00827-x

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  • DOI: https://doi.org/10.1007/s11130-020-00827-x

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