Application of Tamarind Waste Extracts to Improve the Antioxidant Properties of Tamarind Nectars


Tamarind fruits are consumed worldwide and their seeds have an underexploited potential. We assessed the effect of the addition of a freeze-dried aqueous of extract tamarind seed (FAE) at three concentration levels (0.3, 1.15 and 2%) on the antioxidant capacity (DPPH, ABTS, FRAP and ORAC) and concentrations of total phenolic compounds in tamarind pulp. Conditions used to prepare the aqueous extracts were established using multivariate optimization. Moreover, nectars prepared from pulps combined with FAE were subjected to sensory tests. Tamarind fruits from three geographic regions in Brazil (Minas Gerais, São Paulo and Bahia) that were harvested in 2013 and 2014 were used in the study. Generally, the freeze-dried aqueous extracts increased the concentrations of antioxidants in the pulp. The results revealed a positive correlation between the FAE concentration in the pulp and the antioxidant capacity of all samples, particularly samples from Bahia and Minas Gerais, which presented an increase of up to 1,942% in the ABTS method when 2% FAE was incorporated into the pulp, from approximately 40.1 to 209.1 mMTrolox/gdw and 13.4 to 143.4 mMTrolox/gdw, respectively. Sensory tests indicated the satisfactory acceptance and non-distinction between nectar samples to which FAE was or was not added when the FAE concentration was less than 2.3gFAE/L, regardless of the geographic origin of the samples.

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Freeze-dried tamarind seed aqueous extract


2,2-diphenyl-1-picrylhydrazyl; ABTS [2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)]


Ferric reducing antioxidant power


Oxygen radical absorbance capacity


Low density lipoprotein


Total phenolic compounds


Central composite design


Analysis of variance


Solid-to-liquid ratio


Bahia state


Minas Gerais state


São Paulo state


  1. 1.

    Alibabić V, Skender A, Bajramović M et al (2018) Evaluation of morphological, chemical, and sensory characteristics of raspberry cultivars grown in Bosnia and Herzegovina. Turk J Agric For 42:67–74.

    CAS  Article  Google Scholar 

  2. 2.

    Çavuşoĝlu S (2018) Effects of hot water and U&V-C on mineral content changes in two strawberry cultivars stored at different temperatures. Turk J Agric For 42:423–432.

    CAS  Article  Google Scholar 

  3. 3.

    Gündüz K, Özbay H (2018) The effects of genotype and altitude of the growing location on physical, chemical, and phytochemical properties of strawberry. Turk J Agric For 42:145–153.

    CAS  Article  Google Scholar 

  4. 4.

    Wongs-Aree C, Noichinda S (2014) Chapter 10 – postharvest physiology and quality maintenance of tropical fruits. In: Florkowski W, Shewfelt R, Prussia S (eds) Postharvest Handling, 3rd edn. Academic Press, San Diego, pp 275–312.

    Google Scholar 

  5. 5.

    Earling M, Beadle T, Niemeyer ED (2019) Açai berry (Euterpe oleracea) dietary supplements: variations in anthocyanin and flavonoid concentrations, phenolic contents, and antioxidant properties. Plant Foods Hum Nutr 74:421–429.

    CAS  Article  Google Scholar 

  6. 6.

    Paludo MC, Oliveira LF, Hermosín-Gutiérrez I et al (2019) Extracts of peels and seeds of five varieties of Brazilian jabuticaba present high capacity to deactivate reactive species of oxygen and nitrogen. Plant Foods Hum Nutr 74:135–140.

    Article  Google Scholar 

  7. 7.

    Elumalai K, Velmurugan S, Ravi S et al (2015) Facile, eco-friendly and template free photosynthesis of cauliflower like ZnO nanoparticles using leaf extract of Tamarindus indica (L.) and its biological evolution of antibacterial and antifungal activities. Spectrochim Acta A 136:1052–1057.

    CAS  Article  Google Scholar 

  8. 8.

    Komutarin T, Azadi S, Butterworth L, Keil D, Chitsomboon B, Suttajit M, Meade BJ (2004) Extract of the seed coat of Tamarindus indica inhibits nitric oxide production by murine macrophages in vitro and in vivo. Food Chem Toxicol 42:649–658.

    CAS  Article  PubMed  Google Scholar 

  9. 9.

    Tsuda T, Makino Y, Kato H et al (1993) Screening for Antioxidative activity of edible pulses. Biosci Biotechnol Biochem 57:1606–1608.

    CAS  Article  Google Scholar 

  10. 10.

    Tsuda T, Watanabe M, Ohshima K et al (1994) Antioxidative components isolated from the seed of tamarind (Tamarindus indica L.). J Agric Food Chem 42:2671–2674.

    CAS  Article  Google Scholar 

  11. 11.

    Maiti R, Jana D, Das UK, Ghosh D (2004) Antidiabetic effect of aqueous extract of seed of Tamarindus indica in streptozotocin-induced diabetic rats. J Ethnopharmacol 92:85–91.

    CAS  Article  PubMed  Google Scholar 

  12. 12.

    De D, Chatterjee K, Jana K et al (2013) Searching for antihyperglycemic phytomolecules through bioassay-guided solvent fractionation and subfractionation from hydro-methanolic (2:3) extract of Tamarindus indica Linn. seeds in streptozotocin-induced diabetic rat. Biomark Genom Med 5:164–174.

    Article  Google Scholar 

  13. 13.

    Tsoi B, Yi RN, Cao LF, Li SB, Tan RR, Chen M, Li XX, Wang C, Li YF, Kurihara H, He RR (2015) Comparing antioxidant capacity of purine alkaloids: a new, efficient trio for screening and discovering potential antioxidants in vitro and in vivo. Food Chem 176:411–419.

    CAS  Article  PubMed  Google Scholar 

  14. 14.

    Apak R, Gorinstein S, Böhm V et al (2013) Methods of measurement and evaluation of natural antioxidant capacity/activity (IUPAC technical report). Pure Appl Chem 85:957–998.

    CAS  Article  Google Scholar 

  15. 15.

    Prior RL, Wu X, Schaich K (2005) Standardized methods for the determination of antioxidant capacity and Phenolics in foods and dietary supplements. J Agric Food Chem 53:4290–4302.

    CAS  Article  PubMed  Google Scholar 

  16. 16.

    Yoo SH, Shin H, Park M-S (2015) New product development and the effect of supplier involvement. Omega 51:107–120.

    Article  Google Scholar 

  17. 17.

    Razali N, Mat-Junit S, Abdul-Muthalib AF et al (2012) Effects of various solvents on the extraction of antioxidant phenolics from the leaves, seeds, veins and skins of Tamarindus indica L. Food Chem 131:441–448.

    CAS  Article  Google Scholar 

  18. 18.

    Tril U, Fernández-López J, Álvarez JÁP et al (2014) Chemical, physicochemical, technological, antibacterial and antioxidant properties of rich-fibre powder extract obtained from tamarind (Tamarindus indica L.). Ind Crop Prod 55:155–162.

    CAS  Article  Google Scholar 

  19. 19.

    Caliz J, Montes-Borrego M, Triadó-Margarit X, Metsis M, Landa BB, Casamayor EO (2015) Influence of edaphic, climatic, and agronomic factors on the composition and abundance of nitrifying microorganisms in the Rhizosphere of commercial olive crops. PLoS One 10:e0125787.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  20. 20.

    Dahmoune F, Boulekbache L, Moussi K et al (2013) Valorization of Citrus limon residues for the recovery of antioxidants: evaluation and optimization of microwave and ultrasound application to solvent extraction. Ind Crop Prod 50:77–87.

    CAS  Article  Google Scholar 

  21. 21.

    Souza DS, Marques LG, Gomes EB et al (2015) Lyophilization of avocado (Persea americana Mill.): effect of freezing and lyophilization pressure on antioxidant activity, texture, and browning of pulp. Dry Technol 33:194–204.

    Article  Google Scholar 

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The authors thank FAPESP (process number: 2012/06806-4) for the financial support.

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Correspondence to Helena Teixeira Godoy.

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Souza, D.S., Souza, J.D.R.P., Coutinho, J.P. et al. Application of Tamarind Waste Extracts to Improve the Antioxidant Properties of Tamarind Nectars. Plant Foods Hum Nutr 75, 70–75 (2020).

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  • Aqueous extraction
  • Antioxidant capacity
  • Functional beverage
  • Juice
  • PCA (principal component analysis)
  • Sensory analysis
  • Tamarindus indica L.