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Chemical and Nutritional Evaluation of Protein-Rich Ingredients Obtained through a Technological Process from Yellow Lupin Seeds (Lupinus luteus)


In recent years, interest in plant-based proteins has been rising due to ethical and sustainability issues. In this context, the production of protein concentrates and isolates from new plant sources have increased enormously because of their nutritional and techno-functional properties. Therefore, this work describes a pilot process for obtaining protein-rich ingredients from a yellow lupin variety (Lupinus luteus) developed by the Agriaquaculture Nutritional Genomic Center (CGNA). A protein alkaline solubilisation followed by isoelectric precipitation was used as a method for obtaining a protein concentrate (LPC) and isolate (LPI) with 75 and 95% protein, respectively. The changes in the protein quality and chemical composition during the protein concentration process from lupin flour were evaluated. Thus, nutritional parameters such as the amino acids (AAs) profile, essential amino acid index (EAAI), chemical score (CS), the protein efficiency ratio (PER), and digestible indispensable amino acid score (DIAAS) were determined to evaluate the nutritional quality of LPC and LPI. The facile and scalable protein isolation method without a defatting process proposed in this study showed a great protein separation efficiency (PSE), while the amino acids profile was not affected during both the concentration as well as the isolation of proteins. In addition, SDS-PAGE showed that both LPC and LPI mainly contained the high-molecular-weight proteins α and β-conglutins. Both LPC and LPI had a balanced amino acids profile, and arginine was the most predominant amino acid. These results are useful for increasing the use of lupin based-protein ingredients as a potential functional ingredient in the food industry.

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Fig. 1
Fig. 2



Amino acid


Amino acids


Crude protein


Chemical score


Digestible indispensable amino acid score


Essential amino acid


Essential amino acids


Essential amino acid index


Lupin flour


Lupin protein concentrate


Lupin protein isolate


Nitrogen-free extract


Protein efficiency ratio


Protein separation efficiency


Sodium dodecyl sulphate polyacrylamide gel electrophoresis


Standardised ileal digestibility


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This research was supported by CONICYT through project N°R16F1002 (Programa de Fortalecimiento de Centros Regionales) and CONICYT-FONDEF/ ID18I10093. We acknowledge the Regional Government from La Araucanía Region (Chile).

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Correspondence to César Burgos-Díaz.

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Burgos-Díaz, C., Opazo-Navarrete, M., Wandersleben, T. et al. Chemical and Nutritional Evaluation of Protein-Rich Ingredients Obtained through a Technological Process from Yellow Lupin Seeds (Lupinus luteus). Plant Foods Hum Nutr 74, 508–517 (2019).

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  • Lupinus luteus
  • Protein isolate
  • Rich-protein
  • Amino acids
  • Nutritional properties