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Broken Rice as a Potential Functional Ingredient with Inhibitory Activity of Renin and Angiotensin-Converting Enzyme(ACE)

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Abstract

The aim of this work was to evaluate the ability of broken rice, an underutilized industrial by-product, as a potential functional and health promoting ingredient. With this purpose, the ability to inhibit the angiotensin converting enzyme and renin of a rice protein hydrolyzate (RPH) obtained from a high-protein variety of broken rice (var. Nutriar FCAyF) was analyzed (IC50 = 0.87 and 2.7 mg/mL, respectively). RPH was separated by gel permeation chromatography and in a second purification step by RP-HPLC. The sequence of antihypertensive peptides presented in two RP-HPLC fractions was analyzed. Peptides capable of interacting with the active sites of both enzymes were identified. In this study, we demonstrate that the hydrolysis treatment improves functional and biological properties of rice proteins. Protein preparations obtained from a by-product of rice industry, such as broken rice, are a promising ingredient with potentially good biological properties.

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Acknowledgements

The authors acknowledge the contribution of Dra. Nora E. Martínez to this work.

Funding

This work was supported by Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT, Argentina), Project PICT-2012- 0937.

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Authors and Affiliations

  1. Programa Arroz, Facultad de Ciencias Agrarias y Forestales (FCAyF), Universidad Nacional de la Plata, 60 and 119 St. (1900), La Plata, Buenos Aires, Argentina

    María Pinciroli

  2. Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA), Facultad de Ciencias Exactas, Comisión de Investigaciones Científicas (CIC-PBA) and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET- CCT La Plata), Universidad Nacional de La Plata (UNLP), 47 and 116 St. (1900), La Plata, Buenos Aires, Argentina

    Paula Aphalo, Agustina E. Nardo, María C. Añón & Alejandra V. Quiroga

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  1. María Pinciroli
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  2. Paula Aphalo
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  4. María C. Añón
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  5. Alejandra V. Quiroga
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Correspondence to Alejandra V. Quiroga.

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Pinciroli, M., Aphalo, P., Nardo, A.E. et al. Broken Rice as a Potential Functional Ingredient with Inhibitory Activity of Renin and Angiotensin-Converting Enzyme(ACE). Plant Foods Hum Nutr 74, 405–413 (2019). https://doi.org/10.1007/s11130-019-00754-6

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