Sorbus umbellata (Desf.) Fritsch var. umbellata Leaves: Optimization of Extraction Conditions and Investigation Antimicrobial, Cytotoxic, and β-Glucuronidase Inhibitory Potential
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This study aimed to optimize the extraction conditions for Sorbus umbellata (Desf.) Fritsch var. umbellata leaves to maximize the phenolic content and their antioxidant activity and to investigate β-glucuronidase (GUS) enzyme inhibitory, antimicrobial and cytotoxic potentials of the extracts obtained under optimum conditions. The optimum extraction conditions were found to be 78.2 and 79.7% solvent, 73.1 and 71.5 °C, and 89.9 and 88.8 min to maximize phenolic content and antioxidant activity, respectively. Low values of coefficient of variations indicate the high reliability and reproducibility of the conducted extraction experiments. Bioactivity results showed that extracts had cytotoxic effect on the MCF-7 and A549 cells where the highest cell proliferation inhibition was observed for the A549 cell line (71.8% at 150 μg/mL). Staphylococcus aureus showed highest zone of inhibition (19.3 mm) in all bacteria followed by Escherichia coli. Additionally, extracts displayed potential GUS inhibitory activity. In conclusion, Sorbus umbellata leaf extract can be obtained by optimized cost-saving extraction and has a potential bioactivity to be utilized as a food ingredient for high value-added products and/or nutraceuticals development where it can combat oxidative stress and GUS mediated reactive metabolite formation.
KeywordsExtraction Optimization Sorbus umbellata Antioxidant Antibacterial Cytotoxicity
The authors gratefully acknowledge the financial support of the Afyon Kocatepe University Scientific Research Projects Coordinatory Unit (Project No: 12.TEMATIK.05).
Compliance with Ethical Standards
Conflict of Interest
Authors have no conflict of interest to declare.
- 1.Korkut S, Güller B, Aytın A, Kök MS (2009) Turkey’s native wood species: physical and mechanical characterization and surface roughness of rowan. (Sorbus aucuparia L). Wood Res 54:19–30Google Scholar
- 2.Gültekin HC, Alan M (2007) Türkiye’nin üvezleri. Floraplus Dergisi 12:76–82 in TurkishGoogle Scholar
- 3.Fakir H, Korkmaz M, Güller B (2009) Medicinal plant diversity of Western Mediterrenean region in Turkey. J App Bio Sci 3(2):30–40Google Scholar
- 13.Kavak DD (2017) Optimization of extraction time, temperature and solvent concentration for the antioxidant activity and total phenolic content of the Cydonia oblonga mill. leaves. Am-Eurasian J Sustain Agric 11(6):1–6Google Scholar
- 14.Sekikawa C, Kurihara H, Goto K, Takahashi K (2002) Inhibition of β-Glucuronidase by extracts of Chondria crassicaulis. Bulletin of the Faculty of Fisheries, Hokkaido University 53(1):27–30Google Scholar
- 16.Montgomery DC (2001) Design and analysis of experiments. John Wiley and Sons Inc, 5th Ed, New York, USA, pp 218–276Google Scholar
- 18.Khajeh M, Moghaddam MG, Danesh AZ, Khajeh B (2015) Response surface modeling of betulinic acid pre-concentration from medicinal plant samples by miniaturized homogenous liquid–liquid extraction and its determination by high performance liquid chromatography. Arab J Chem 8:400–406. https://doi.org/10.1016/j.arabjc.2013.03.001 CrossRefGoogle Scholar
- 19.Majd MH, Rajaei A, Bashi DS, Mortazavi SA, Bolourian S (2014) Optimization of ultrasonic-assisted extraction of phenolic compounds from bovine pennyroyal (Phlomidoschema parviflorum) leaves using response surface methodology. Ind Crop Prod 57:195–202. https://doi.org/10.1016/j.indcrop.2014.03.031 CrossRefGoogle Scholar
- 22.Olszewska MA (2011) In vitro antioxidant activity and total phenolic content of the inflorescences, leaves and fruits of Sorbus torminalis (L.) Crantz. Acta Pol Pharm 68:945–953Google Scholar
- 25.Samuagam L, Sia CM, Akowuah GA, Okechukwu PN, Yim HS (2013) The effect of extraction conditions on total phenolic content and free radical scavenging capacity of selected tropical fruits’ peel. Health Environ J 4:80–102Google Scholar
- 27.Radojkovic M, Zekovic Z, Jokic S, Vidovic S (2012) Determination of optimal extraction parameters of mulberry leaves using response surface methodology (RSM). Rom Biotech Lett 17:7295–7308Google Scholar
- 30.Jimenez P, Cabrero P, Basterrechea JE, Tejero J, Cordoba-Diaz D, Cordoba-Diaz M, Girbes T (2014) Effects of short-term heating on total polyphenols, anthocyanins antioxidant activity and lectins of different parts of dwarf elder (Sambucus ebulus L.). Plant Foods Hum Nutr 69:168–174CrossRefGoogle Scholar
- 32.Olszewska MA (2008) Separation of quercetin, sexangularetin, kaempferol and isorhamnetin for simultaneous HPLC determination of flavonoid aglycones in inflorescences, leaves and fruits of three Sorbus species. J Pharmaceut Biomed 48:629–635. https://doi.org/10.1016/j.jpba.2008.06.004 CrossRefGoogle Scholar
- 33.Rutkowska M, Owczarek A, Kolodziejczyk-Czepas J, Michel P, Piotrowska DG, Kapusta P, Nowak P, Olszewska MA (2019) Identification of bioactivity markers of Sorbus domestica leaves in chromatographic, spectroscopic and biological capacity tests: application for the quality control. Phytochem Lett 30:278–287CrossRefGoogle Scholar