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Plant Foods for Human Nutrition

, Volume 74, Issue 3, pp 364–369 | Cite as

Sorbus umbellata (Desf.) Fritsch var. umbellata Leaves: Optimization of Extraction Conditions and Investigation Antimicrobial, Cytotoxic, and β-Glucuronidase Inhibitory Potential

  • Dilek Demirbuker KavakEmail author
  • Bilge Akdeniz
Original Paper
  • 118 Downloads

Abstract

This study aimed to optimize the extraction conditions for Sorbus umbellata (Desf.) Fritsch var. umbellata leaves to maximize the phenolic content and their antioxidant activity and to investigate β-glucuronidase (GUS) enzyme inhibitory, antimicrobial and cytotoxic potentials of the extracts obtained under optimum conditions. The optimum extraction conditions were found to be 78.2 and 79.7% solvent, 73.1 and 71.5 °C, and 89.9 and 88.8 min to maximize phenolic content and antioxidant activity, respectively. Low values of coefficient of variations indicate the high reliability and reproducibility of the conducted extraction experiments. Bioactivity results showed that extracts had cytotoxic effect on the MCF-7 and A549 cells where the highest cell proliferation inhibition was observed for the A549 cell line (71.8% at 150 μg/mL). Staphylococcus aureus showed highest zone of inhibition (19.3 mm) in all bacteria followed by Escherichia coli. Additionally, extracts displayed potential GUS inhibitory activity. In conclusion, Sorbus umbellata leaf extract can be obtained by optimized cost-saving extraction and has a potential bioactivity to be utilized as a food ingredient for high value-added products and/or nutraceuticals development where it can combat oxidative stress and GUS mediated reactive metabolite formation.

Keywords

Extraction Optimization Sorbus umbellata Antioxidant Antibacterial Cytotoxicity 

Notes

Acknowledgements

The authors gratefully acknowledge the financial support of the Afyon Kocatepe University Scientific Research Projects Coordinatory Unit (Project No: 12.TEMATIK.05).

Compliance with Ethical Standards

Conflict of Interest

Authors have no conflict of interest to declare.

Supplementary material

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ESM 1 (DOCX 18 kb)
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ESM 2 (DOCX 13 kb)
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ESM 6

Response surface for the effects of independent variables on total phenolic content TP (R1) and radical scavenging activity AA% (R2). (a) R1 according to solvent concentration and extraction temperature, (b) R1 according to extraction temperature and time, (c) R2 according to extraction temperature and time, (d) R2 according to solvent concentration and extraction temperature (PNG 4166 kb)

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High Resolution (TIF 375 kb)
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ESM 7 HPLC chromatogram of Sorbus leaf extract (3:catechin hydrate, 5:chlorogenic acid, 6:caffeic acid, 7:epicatechin, 10:p-coumaric acid, 11:ferulic acid, 15: rutin, 21:quercetin) (DOCX 18 kb)
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β-Glucuronidase inhibitory effects of Sorbus leaf extract (5–150 μg/mL) (PNG 1050 kb)

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High Resolution (TIF 413 kb)
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Cytotoxicity results in terms of cell proliferation inhibiton (%) of Sorbus leaf extract (5–150 μg/mL) against MCF-7 breast adenocarcinoma cell line, and 549 lung adenocarcinoma cell line (PNG 1370 kb)

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High Resolution (TIF 456 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Engineering Faculty, Food Engineering DepartmentAfyon Kocatepe UniversityAfyonkarahisarTurkey

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