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Selective and Apoptotic Action of Ethanol Extract of Annona cherimola Seeds against Human Stomach Gastric Adenocarcinoma Cell Line AGS

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Abstract

Annona cherimola is a tree belonging to the family Annonacea, whose fruit (cherimoya) is very desirable, but its seeds are considered waste. Present in these seeds are compounds that have been described as selective antiproliferative agents for cancer cells. The aim of this study was to evaluate the antiproliferative activity of ethanol macerate extract (EMCHS) obtained from A. cherimola seeds against the human stomach gastric adenocarcinoma (AGS) cell line and the normal human gastric epithelial cell line (GES-1). The EMCHS extract presented an IC50 of 80.43 μg/mL in AGS cells, and a selectivity index (SI) of 3.5-fold higher than that of cisplatin. In addition, the EMCHS extract showed apoptotic activity in AGS cells since 50 μg/mL. Overxpression of PUMA gene in both cells demonstrate that EMCHS activate the apoptotic route. Future studies should be carried out to elucidate anticancer activity of EMCHS in vivo. This work represents the first showing antiproliferative effects of crude extracts obtained from seeds of A. cherimola in AGS cells.

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Abbreviations

EMCHS :

Ethanol macerate of A. cherimola seeds

AGS :

Human gastric adenocarcinoma cell line

GES-1 :

Normal human gastric epithelial cell line 1

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Acknowledgements

We thank Mr. Eduardo Gutiérrez and Pablo Villalobos for providing the necessary facilities to collect the fruits of A. cherimola in the Huerto de Chirimoyos of Universidad de La Serena. We are also very grateful to Ms. Karla Gonzalez for their valuable help with the TLC. We thank the Department of Food Engineering of Universidad de La Serena for providing access to their laboratories during several stages of this study. Giuliano Bernal thank Dr. Lorena Lobos-Gonzalez and Dr. Luis Burzio (AGS) and Dr. Dawit Kidane-Mulat (GES-1) for kindly provide the human cells used in this study.

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Authors and Affiliations

  1. Laboratorio de Silvogenómica y Biotecnología, Departamento de Biología, Facultad de Ciencias Universidad de La Serena, Avenida Raúl Bitrán 1305, Casilla 599, 1700000, La Serena, Chile

    Johan Macuer-Guzmán & Cristian Ibáñez

  2. Laboratorio de Biología Molecular y Celular del Cáncer, Departamento de Ciencias Biomédicas, Facultad de Medicina, Universidad Católica del Norte, Larrondo 1281, 1781421, Coquimbo, Chile

    Giuliano Bernal & Sebastian Ramírez-Rivera

  3. Laboratorio de Fitoquímica y Productos Naturales, Departamento de Química, Facultad de Ciencias, Universidad de La Serena, Avenida Raúl Bitrán 1305, Casilla 599, 1700000, La Serena, Chile

    Fabiola Jamett-Díaz

Authors
  1. Johan Macuer-Guzmán
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  2. Giuliano Bernal
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  3. Fabiola Jamett-Díaz
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  4. Sebastian Ramírez-Rivera
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  5. Cristian Ibáñez
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Correspondence to Cristian Ibáñez.

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Macuer-Guzmán, J., Bernal, G., Jamett-Díaz, F. et al. Selective and Apoptotic Action of Ethanol Extract of Annona cherimola Seeds against Human Stomach Gastric Adenocarcinoma Cell Line AGS. Plant Foods Hum Nutr 74, 322–327 (2019). https://doi.org/10.1007/s11130-019-00742-w

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