Prebiotics are regarded as the non-digestible food constituents that are selectively consumed by health-promoting bacteria (probiotics). In fact, a number of active metabolites is released due to intensive interaction between prebiotics and probiotics in the gut which exert local and systemic beneficial effects including regulation of intestinal disorders and modulation of host immunity. Turmeric is one of the most important medicinal herbaceous that is derived from Curcuma longa rhizome. Curcumin is a well-recognized component of turmeric which contributes to the prevention of multiple inflammatory diseases. Despite curcumin as a well-known compound, few researches have focused on the turmeric extract (TE) and its potential as prebiotic and anti-inflammatory compound. The aim of this study was to evaluate the prebiotic potential and some functional-structural properties of TE. The Fourier-transform-infrared spectroscopy (FTIR) spectrum of TE showed identical peaks that belonged to β configuration in pyranose and glycosidic bonds. High performance liquid chromatography (HPLC) analysis revealed the presence of potent phenolic and flavonoid anti-oxidants and curcuminoids, and some functional monosaccharides. TE demonstrated excellent resistance to artificial human gastric and intestine juice compared to the standard prebiotic (inulin) (p ≤ 0.05). Interestingly, our time course experiment showed that TE not only is digested by probiotics including Lactobacillus rhamnosus GG (LGG) and Bifidobacterium animalis BB12, but also supports the growth of these bacteria even after 72 h (p ≤ 0.05). To our knowledge, this is the first report evaluating prebiotic potential of TE and exploring its suppressive effects on LPS induced IL-8 production in HT29-19A cell line.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Price excludes VAT (USA)
Tax calculation will be finalised during checkout.
Lactobacillus rhamnosus GG
Mangell P, Nejdfors P, Wang M, Ahrné S, Weström B, Thorlacius H, Jeppsson B (2002) Lactobacillus plantarum 299v inhibits Escherichia coli-induced intestinal permeability. Dig Dis Sci 47:511–516. https://doi.org/10.1023/A:1017947531536
Hsieh CY, Osaka T, Moriyama E, Date Y, Kikuchi J, Tsuneda S (2015) Strengthening of the intestinal epithelial tight junction by Bifidobacterium bifidum. Phys Rep 3:1–17. https://doi.org/10.14814/phy2.12327
Blackwood BP, Yuan CY, Wood DR, Nicolas JD, Grothaus JS, Hunter CJ (2017) Probiotic Lactobacillus species strengthen intestinal barrier function and tight junction integrity in experimental necrotizing enterocolitis. J Probiotics Heal 5:457–464. https://doi.org/10.4172/2329-8901.1000159
Koh JH, Kim WU (2017) Dysregulation of gut microbiota and chronic inflammatory disease: from epithelial defense to host immunity. Exp Mol Med 49:e337. https://doi.org/10.1038/emm.2017.55
Bindels LB, Delzenne NM, Cani PD, Walter J (2015) Opinion: towards a more comprehensive concept for prebiotics. Nat Rev Gastroenterol Hepatol 12:303–310. https://doi.org/10.1038/nrgastro.2015.47
Tomé-Carneiro J, Visioli F (2016) Polyphenol-based nutraceuticals for the prevention and treatment of cardiovascular disease: review of human evidence. Phytomedicine 23:1145–1174. https://doi.org/10.1016/j.phymed.2015.10.018
Zeng Z, Shen ZL, Zhai S, Xu JL, Liang H, Shen Q, Li QY (2017) Transport of curcumin derivatives in Caco-2 cell monolayers. Eur J Pharm Biopharm 117:123–131. https://doi.org/10.1016/j.ejpb.2017.04.004
Aggarwal BB, Yuan W, Li S, Gupta SC (2013) Curcumin-free turmeric exhibits anti-inflammatory and anticancer activities: identification of novel components of turmeric. Mol Nutr Food Res 57:1529–1542. https://doi.org/10.1002/mnfr.201200838
Menon VP, Sudheer AR (2007) Antioxidant and anti-inflammatory properties of curcumin. Adv Exp Med Biol 595:105–125. https://doi.org/10.1007/978-0-387-46401-5_3
Chen K, Liang N, Luo X, Zhang TC (2013) Lactobacillus acidophilus strain suppresses the transcription of proinflammatory-related factors in human HT-29 cells. J Microbiol Biotechnol 23:64–68. https://doi.org/10.4014/jmb.1208.04067
Bai AP, Ouyang Q, Zhang W, Wang CH, Li SF (2004) Probiotics inhibit TNF-α-induced interleukin-8 secretion of HT29 cells. World J Gastroenterol 10:455–457. https://doi.org/10.3748/wjg.v10.i3.455
Verhoeckx K, Cotter P, López-Expósito I, et al (2015) The impact of food bioactives on health: in vitro and ex vivo models. COST Action FA1005, Springer. https://doi.org/10.1007/978-3-319-16104-4
Arboleya S, Watkins C, Stanton C, Ross RP (2016) Gut bifidobacteria populations in human health and aging. Front Microbiol 7:1–9. https://doi.org/10.3389/fmicb.2016.01204
Kozarski M, Klaus A, Jakovljevic D, Todorovic N, Niksic M, Vrvic MM, van Griensven LJLD (2014) Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification. Food Res Int 64:53–64. https://doi.org/10.1016/j.foodres.2014.05.075
Xu RB, Yang X, Wang J, Zhao HT, Lu WH, Cui J, Cheng CL, Zou P, Huang WW, Wang P, Li WJ, Hu XL (2012) Chemical composition and antioxidant activities of three polysaccharide fractions from pine cones. Int J Mol Sci 13:14262–14277. https://doi.org/10.3390/ijms131114262
Wong JM, Jenkins DJ (2007) Carbohydrate digestibility and metabolic effects. J Nutr 137:2539–2546. https://doi.org/10.1093/jn/137.11.2539S
Azmi AFMN, Mustafa S, Hashim DM, Manap YA (2012) Prebiotic activity of polysaccharides extracted from Gigantochloa Levis (buluh beting) shoots. Molecules 17:1635–1651. https://doi.org/10.3390/molecules17021635
Tadayoni M, Sheikh-Zeinoddin M, Soleimanian-Zad S (2015) Isolation of bioactive polysaccharide from acorn and evaluation of its functional properties. Int J Biol Macromol 72:179–184. https://doi.org/10.1016/j.ijbiomac.2014.08.015
Akbari-Alavijeh S, Soleimanian-Zad S, Sheikh-Zeinoddin M, Hashmi S (2018) Pistachio hull water-soluble polysaccharides as a novel prebiotic agent. Int J Biol Macromol 107:808–816. https://doi.org/10.1016/j.ijbiomac.2017.09.049
Wichienchot S, Prasertsan P, Hongpattarakere T, Gibson GR, Rastall RA (2006) In vitro fermentation of mixed linkage gluco-oligosaccharides produced by Gluconobacter. Curr Issues Intest Microbiol 7:7–12
Hansawasdi C, Kurdi P (2017) Potential prebiotic oligosaccharide mixtures from acidic hydrolysis of rice bran and cassava pulp. Plant Foods Hum Nutr 72:396–403. https://doi.org/10.1007/s11130-017-0636-z
Wichienchot S, Jatupornpipat M, Rastall RA (2010) Oligosaccharides of pitaya (dragon fruit) flesh and their prebiotic properties. Food Chem 120:850–857. https://doi.org/10.1016/j.foodchem.2009.11.026
den Besten G, van Eunen K, Groen AK, Venema K, Reijngoud DJ, Bakker BM (2013) The role of short-chain fatty acids in the interplay between diet, gut microbiota, and host energy metabolism. J Lipid Res 54:2325–2340. https://doi.org/10.1194/jlr. R036012
Yadav VS, Mishra KP, Singh DP, Mehrotra S, Singh VK (2005) Immunomodulatory effects of curcumin. Immunopharmacol Immunotoxicol 27:485–497. https://doi.org/10.1080/08923970500242244
Guo Y, Shu L, Zhang C, Su ZY, Kong ANT (2015) Curcumin inhibits anchorage-independent growth of HT29 human colon cancer cells by targeting epigenetic restoration of the tumor suppressor gene DLEC1. Biochem Pharmacol 94:69–78. https://doi.org/10.1016/j.bcp.2015.01.009
Altamimi M, Abdelhay O, Rastall RA (2016) Effect of oligosaccharides on the adhesion of gut bacteria to human HT-29 cells. Anaerobe 39:136–142. https://doi.org/10.1016/j.anaerobe.2016.03.010
Duary RK, Batish VK, Grover S (2014) Immunomodulatory activity of two potential probiotic strains in LPS-stimulated HT-29 cells. Genes Nutr 9(398):398. https://doi.org/10.1007/s12263-014-0398-2
Lehmann S, Hiller J, Van Bergenhenegouwen J et al (2015) In vitro evidence for immune-modulatory properties of non-digestible oligosaccharides: direct effect on human monocyte derived dendritic cells. PLoS One 10:1–15. https://doi.org/10.1371/journal.pone.0132304
Serafini F, Strati F, Ruas-Madiedo P, Turroni F, Foroni E, Duranti S, Milano F, Perotti A, Viappiani A, Guglielmetti S, Buschini A, Margolles A, van Sinderen D, Ventura M (2013) Evaluation of adhesion properties and antibacterial activities of the infant gut commensal Bifidobacterium bifidum PRL2010. Anaerobe 21:9–17. https://doi.org/10.1016/j.anaerobe.2013.03.003
Candela M, Perna F, Carnevali P, Vitali B, Ciati R, Gionchetti P, Rizzello F, Campieri M, Brigidi P (2008) Interaction of probiotic Lactobacillus and Bifidobacterium strains with human intestinal epithelial cells: adhesion properties, competition against enteropathogens and modulation of IL-8 production. Int J Food Microbiol 125:286–292. https://doi.org/10.1016/j.ijfoodmicro.2008.04.012
Tan Y-F, Li H-L, Lai W-Y, Zhang J-Q (2013) Crude dietary polysaccharide fraction isolated from jackfruit enhances immune system activity in mice. J Med Food 16:663–668. https://doi.org/10.1089/jmf.2012.2565
Conflict of Interest
This research was supported by Isfahan University of Technology (IUT) and Utrecht University. The authors declare that they have no conflict of interest.
Human and Animal Studies
This article does not contain any studies with human or animal subjects.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic supplementary material
About this article
Cite this article
Ghiamati Yazdi, F., Soleimanian-Zad, S., van den Worm, E. et al. Turmeric Extract: Potential Use as a Prebiotic and Anti-Inflammatory Compound?. Plant Foods Hum Nutr 74, 293–299 (2019). https://doi.org/10.1007/s11130-019-00733-x