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Guava (Psidium guajava L. cv. Red Suprema) Crude Extract Protect Human Dermal Fibroblasts against Cytotoxic Damage Mediated by Oxidative Stress


We analyzed guava fruits (Psidium guajava L. cv. Red Suprema) from Cuba to determine their chemical composition, total antioxidant capacity, as well as their protective effect against oxidative damage using an in vitro model of human dermal fibroblasts. The guava fruit is a natural source of bioactive compounds, such as polyphenols, vitamin C, folates and beta carotenes with proven health benefits. Human dermal fibroblasts were pre-incubated with different concentrations of guava crude extract and then subjected to oxidative stress using the AAPH stressor. The number of apoptotic and dead cells, as well as the markers of oxidative damage such as lipid and protein oxidation significantly decreased when cells were pre-incubated with guava crude extract and then exposed to the stressor. The activity of antioxidant enzymes also improved when cells were pre-incubated with guava crude extract in comparison to cells subjected to stress without prior pre-incubation with the guava extract. The results obtained in this study highlight the health benefits of guava regarding oxidative stress, proving it to be an important source of bioactive compounds associated with important biological properties.

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Fig. 1



2,2′-azobis(2-amidinopropane) dihydrochloride






Guava crude extract


Human dermal fibroblasts, adult


Superoxide dismutase


Total antioxidant capacity


Thiobarbituric acid reactive substances


Total phenolic content


Total flavonoid content


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Correspondence to Francesca Giampieri or Maurizio Battino.

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Alvarez-Suarez, J.M., Giampieri, F., Gasparrini, M. et al. Guava (Psidium guajava L. cv. Red Suprema) Crude Extract Protect Human Dermal Fibroblasts against Cytotoxic Damage Mediated by Oxidative Stress. Plant Foods Hum Nutr 73, 18–24 (2018).

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  • Guava fruit
  • Antioxidant capacity
  • Human dermal fibroblast
  • Oxidative stress