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Increased Postharvest Life of TomLox B Silenced Mutants of Tomato (Solanum lycopersicum) Var. TA234

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Abstract

A healthy lifestyle includes fruits and vegetables consumption. Tomato is one of the most consumed vegetables, although it is susceptible to physical damage through postharvest handling, thus leading to important losses. Softening is an important variable during tomato ripening; excessive softening is undesirable and leads to postharvest losses. TomloxB plays an important role in ripening, mainly in the loss of cellular integrity caused by fatty acids released from the lipid matrix of membranes that initiate oxidative deterioration, which is in turn carried into senescence. In order to increase postharvest life, we produced transgenic tomato plants via Rhizobium radiobacter with tomato lipoxygenase B (TomloxB) antisense constructs under control of the cauliflower mosaic virus (CaMV) 35S promoter. Lipoxygenase activity and firmness were measured in tomato fruit and the fatty acids profile was determined. Transgenic fruits were maintained for 40 days at room temperature in optimal conditions, whereas wild type fruits remained in similar conditions for only six days. Firmness in pink and red stages was significantly lower in wild type fruits than in two transgenic lines. Linolenic acid was the most important fatty acid consumed by lipoxygenase in both turning and pink stages of ripening. Lipoxygenase activity was smaller in transformed fruits in comparison with the wild type. These results suggest that silencing the TomloxB gene promoted significant changes in the physiology of transformed tomatoes, being the increase in postharvest life the most important.

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Abbreviations

TomloxB:

Lipoxygenase B in tomato fruit

CaMV :

Cauliflower mosaic virus

LOX:

Lipoxygenase

PUFAs:

Polyunsaturated fatty acids

NPTII:

Neomycin phosphotransferase gene

UIDA:

β-Glucuronidase gene

PCR:

Polymerase chain reaction

RT-PCR:

Real time polymerase chain reaction

FAME:

Fatty acids methyl ester

ROS :

Reactive oxygen species

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Acknowledgments

The authors gratefully acknowledge to CONACyT for the graduate scholarship for the first author (232926). We thankfully acknowledge the technical assistance of Ana Gloria Vallejo Vargas and Diana Zavala Navarro.

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Authors and Affiliations

  1. Unidad de Investigación y Desarrollo en Alimentos (UNIDA) del Instituto Tecnológico de Veracruz, Veracruz, Veracruz, Mexico

    Elizabeth León-García, Gilber Vela-Gutiérrez, Oscar A. Del Ángel-Coronel, Cristobal Torres-Palacios, Javier De La Cruz-Medina & Hugo Sergio García

  2. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV) Irapuato, Guanajuato, Mexico

    Miguel A. Gómez-Lim

Authors
  1. Elizabeth León-García
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  2. Gilber Vela-Gutiérrez
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  3. Oscar A. Del Ángel-Coronel
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  4. Cristobal Torres-Palacios
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  5. Javier De La Cruz-Medina
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  6. Miguel A. Gómez-Lim
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  7. Hugo Sergio García
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Corresponding authors

Correspondence to Miguel A. Gómez-Lim or Hugo Sergio García.

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León-García, E., Vela-Gutiérrez, G., Del Ángel-Coronel, O.A. et al. Increased Postharvest Life of TomLox B Silenced Mutants of Tomato (Solanum lycopersicum) Var. TA234. Plant Foods Hum Nutr 72, 380–387 (2017). https://doi.org/10.1007/s11130-017-0629-y

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  • DOI: https://doi.org/10.1007/s11130-017-0629-y

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