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Biological Activity of Conventional and Organic Pomegranate Juices: Antioxidant and Antimutagenic Potential


None of the health claims about pomegranate juices has been approved yet by European Food Safety Authority (EFSA). There is a general perception among consumers that organic foods are healthier, tastier, and more nutritive than the conventional products. The aim of this research was to study the differences in the biological activity between ready-for-consumption juices obtained from pomegranates fruits grown under conventional and organic agricultural practices. Antioxidant activity has been evaluated by three methods (DPPH, ABTS+, and FRAP), together with the total contents of phenolics and punicalagin (HPLC-DAD); besides, the Ames test was used to evaluate the antimutagenic potential of the juices. Pomegranate juice, either from conventionally or organically grown fruits, was antimutagenic (mean of 51 and 90 % for Salmonella typhimurium TA100 and TA98, respectively) and it was capable of protecting DNA from both, base-pair or frame-shift type of mutations. In fact, the antimutagenicity of conventional pomegranate juice was higher than that achieved by the organic sample; this finding was linked to a higher punicalagin content (201 and 104 mg L−1 for conventional and organic juices, respectively).

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  1. 1.

    Orgil O, Schwartz E, Baruch L, Matityahu I, Mahajna J, Amir R (2014) The antioxidative and anti-proliferative potential of non-edible organs of the pomegranate fruit and tree. LWT - Food Sci Technol 58(2):571–577

    CAS  Article  Google Scholar 

  2. 2.

    Hamoud S, Hayek T, Volkova N, Attias J, Moscoviz D, Rosenblat M, Aviram M (2014) Pomegranate extract (POMx) decreases the atherogenicity of serum and of human monocyte-derived macrophages (HMDM) in simvastatin-treated hypercholesterolemic patients: a double-blinded, placebo-controlled, randomized, prospective pilot study. Atherosclerosis 232(1):204–210

    CAS  Article  Google Scholar 

  3. 3.

    Ordoudi SA, Mantzouridou F, Daftsiou E, Malo C, Hatzidimitriou E, Nenadis N, Tsimidou MZ (2014) Pomegranate juice functional constituents after alcoholic and acetic acid fermentation. J Funct Foods 8:161–168

    CAS  Article  Google Scholar 

  4. 4.

    Zahin M, Ahmad I, Gupta RC, Aqil F (2014) Punicalagin and ellagic acid demonstrate antimutagenic activity and inhibition of benzo[a]pyrene induced DNA adducts. Biomed Res Int 2014:467465

  5. 5.

    Seeram N, Lee R, Hardy M, Heber D (2005) Rapid large scale purification of ellagitannins from pomegranate husk, a by-product of the commercial juice industry. Sep Purif Technol 41(1):49–55

    CAS  Article  Google Scholar 

  6. 6.

    Paredes-López O, Cervantes-Ceja ML, Vigna-Pérez M, Hernández-Pérez T (2010) Berries: improving human health and healthy aging, and promoting quality life - a review. Plant Foods Hum Nutr 65(3):299–308

    Article  Google Scholar 

  7. 7.

    Nuncio-Jáuregui N, Nowicka P, Munera-Picazo S, Hernández F, Carbonell-Barrachina AA, Wojdyło A (2015) Identification and quantification of major derivatives of ellagic acid and antioxidant properties of thinning and ripe Spanish pomegranates. J Funct Foods 12:354–364

    Article  Google Scholar 

  8. 8.

    Cavallini G, Dachà M, Potenza L, Ranieri A, Scattino C, Castagna A, Bergamini E (2014) Use of red blood cell membranes to evaluate the antioxidant potential of plant extracts. Plant Foods Hum Nutr 69(2):108–114

    CAS  Article  Google Scholar 

  9. 9.

    Mastrodi Salgado J, Ferreira TRB, de Oliveira Biazotto F, dos Santos Dias CT (2012) Increased antioxidant content in juice enriched with dried extract of pomegranate (Punica granatum) peel. Plant Foods Hum Nutr 67(1):39–43

    Article  Google Scholar 

  10. 10.

    Les F, Prieto JM, Arbonés-Mainar JM, Valero MS, López V (2015) Bioactive properties of commercialised pomegranate (Punica granatum) juice: antioxidant, antiproliferative and enzyme inhibiting activities. Food Funct 6:2049–2057

    CAS  Article  Google Scholar 

  11. 11.

    Sánchez M, Grande I, Gil JM, Gracia A (1998) Evaluación del potencial de mercado de los productos de agricultura ecológica. Rev Esp Invest Market, ESIC (2):135–150

  12. 12.

    Magkos F, Arvaniti F, Zampelas A (2006) Organic food: buying more safety or just peace of mind? A critical review of the literature. Crit Rev Food Sci Nutr 46:23–56

    Article  Google Scholar 

  13. 13.

    Asami DK, Hong Y-J, Barrett DM, Mitchell AE (2003) Comparison of the total phenolic and ascorbic acid content of freeze-dried and air-dried marionberry, strawberry, and corn grown using conventional, organic, and sustainable agricultural practices. J Agric Food Chem 51:1237–1241

    CAS  Article  Google Scholar 

  14. 14.

    de Boer A, Bast A (2015) International legislation on nutrition and health claims. Food Policy 55:61–70

    Article  Google Scholar 

  15. 15.

    Sjölin K (2006) Nutrition and Health Claims. The proposed regulation on nutrition and health claims in relation to CODEX. IP/A/ENVI/NT/2006–04. European Parliament’s Committee on the Environment, Public Health and Food Safety, Brussels, Belgium

  16. 16.

    EU (2016) EU register of nutrition and health claims made on foods. Accessed 13–06–2016

  17. 17.

    Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. LWT - Food Sci Technol 28:25–30

    CAS  Article  Google Scholar 

  18. 18.

    Benzie IFF, Szeto YT (1999) Total antioxidant capacity of teas by the ferric reducing/antioxidant power assay. J Agric Food Chem 47:633–636

    CAS  Article  Google Scholar 

  19. 19.

    Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26(9–10):1231–1237

    CAS  Article  Google Scholar 

  20. 20.

    Gao X, Ohlander M, Jeppsson N, Björk L, Trajkovski V (2000) Changes in antioxidant effects and their relationship to phytonutrients in fruits of sea buckthorn (Hippophae rhamnoides L.) during maturation. J Agric Food Chem 48:1485–1490

    CAS  Article  Google Scholar 

  21. 21.

    Maron DM, Ames BN (1983) Revised methods for the Salmonella mutagenicity test. Mutat Res-Environ Muta 113(3–4):173–215

    CAS  Article  Google Scholar 

  22. 22.

    Gąstoł M, Świątkiewicz I, Krośniak M (2011) Organic versus conventional - a comparative study on quality and nutritional value of fruit and vegetable juices. Biol Agric Hortic 27(3–4):310–319

    Google Scholar 

  23. 23.

    Calín-Sánchez Á, Figiel A, Hernández F, Melgarejo P, Lech K, Carbonell-Barrachina ÁA (2013) Chemical composition, antioxidant capacity, and sensory quality of pomegranate (Punica granatum L.) arils and rind as affected by drying method. Food Bioprocess Technol 6:1644–1654

    Article  Google Scholar 

  24. 24.

    Mena P, Martí N, Saura D, Valero M, García-Viguera C (2013) Combinatory effect of thermal treatment and blending on the quality of pomegranates juices. Food Bioprocess Technol 6:3186–3199

    CAS  Article  Google Scholar 

  25. 25.

    Wasila H, Li X, Liu L, Ahmad I (2014) Comparing phenolics composition and antioxidant activities of different pomegranate products. Pak J Agric Sci 51(1):167–174

    Google Scholar 

  26. 26.

    Negi PS, Jayaprakasha GK, Jena BS (2003) Antioxidant and antimutagenic activities of pomegranate peel extracts. Food Chem 80(3):393–397

    CAS  Article  Google Scholar 

  27. 27.

    Dassprakash MV, Arun R, Abraham SK, Premkumar K (2012) In vitro and in vivo evaluation of antioxidant and antigenotoxic potential of leaf extract. Pharm Biol 50(12):1523–1530

    Article  Google Scholar 

  28. 28.

    Pellati F, Bruni R, Righi D, Grandini A, Tognolini M, Pio Prencipe F, Poli F, Benvenuti S, Del Rio D, Rossi D (2013) Metabolite profiling of polyphenols in a Terminalia chebula Retzius ayurvedic decoction and evaluation of its chemopreventive activity. J Ethnopharm 147:277–285

    CAS  Article  Google Scholar 

  29. 29.

    Londhe JS, Devasagayam TPA, Foo LY, Shastry P, Ghaskadbi SS (2012) Geraniin and amariin, ellagitannins from Phyllanthus amarus, protect liver cells against ethanol induced cytotoxicity. Fitoterapia 83(8):1562–1568

    CAS  Article  Google Scholar 

  30. 30.

    Kondo H, Hashizume K, Shibuya Y, Hase T, Murase T (2011) Identification of diacylglycerol acyltransferase inhibitors from Rosa centifolia petals. Lipids 46(8):691–700

    CAS  Article  Google Scholar 

  31. 31.

    Kasimsetty SG, Bialonska D, Reddy MK, Thornton C, Willett KL, Ferreira D (2009) Effects of pomegranate chemical constituents/intestinal microbial metabolites on CYP1B1 in 22Rv1 prostate cancer cells. J Agric Food Chem 57(22):10636–10644

    CAS  Article  Google Scholar 

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Author AACB was funded by the Spanish Ministry of Education, Culture and Sports, through fellowship PRX15/00352.

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Correspondence to A. Burgos-Hernández.

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The authors declare that they have no conflict of interest.

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Cano-Lamadrid, M., Marhuenda-Egea, F.C., Hernández, F. et al. Biological Activity of Conventional and Organic Pomegranate Juices: Antioxidant and Antimutagenic Potential. Plant Foods Hum Nutr 71, 375–380 (2016).

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  • Aflatoxin B1
  • Ames test
  • Organic juice
  • Punica granatum
  • Punicalagin
  • Mollar de Elche