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Comparative Study of Phenolic Profile, Antioxidant Capacity, and Color-composition Relation of Roselle Cultivars with Contrasting Pigmentation

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Abstract

Roselle is a plant that accumulates anthocyanins significantly, hence its importance as food coloring and as a source of antioxidant compounds for human health. This study was aimed to determine phenolic composition and antioxidant capacity of methanolic extracts, and beverages obtained from native roselle cultivars in Mexico (Negra, Sudan, Rosa and Blanca) with different degrees of pigmentation, and to establish the color-composition relationship. Chromatographic methods were used to determine phenolic compounds: flavanols, flavonols, benzoic, hibiscus and phenolic acids as well as two main anthocyanins (cyanidin 3-sambubioside and delphinidin 3-sambubioside). The antioxidant capacity was evaluated by ABTS and FRAP assays. Tristimulus colorimetry showed to be a useful technique to determine the color-composition relationship, leading to equations that allowed to predict anthocyanin content of roselle (R > 0.84). Also, a stepwise linear discriminant analysis (SLDA) was developed in order to classify roselle cultivars. The obtained mathematical model could be an important tool to be used in colorimetric characterization of functional compounds used in food processing.

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Abbreviations

DIA:

digital image analysis

Dp3Sa:

delphinidin-3-sambubioside

Cy3Sa:

cyanidin-3-sambubioside

LDA:

linear discriminant analysis

SLDA:

stepwise linear discriminant analysis

TA:

total anthocyanins

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Acknowledgments

Authors thank to Colegio Superior de Agricultura del Estado de Guerrero (CSAEGRO) and Quintín Obispo González for biological samples. Camelo-Méndez G.A also acknowledges to CONACYT-Mexico and SIP-IPN scholarships.

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Authors and Affiliations

  1. Instituto Politécnico Nacional, CEPROBI, Carretera Yautepec-Jojutla Km. 6, Calle CEPROBI 8, Col. San Isidro, Yautepec, Morelos, Mexico, C.P. 62731

    Gustavo A. Camelo-Méndez, Pablo E. Vanegas-Espinoza & Alma A. Del Villar-Martínez

  2. Food Colour & Quality Laboratory, Department Nutrition & Food Science, Facultad de Farmacia, Universidad de Sevilla, 41012, Sevilla, Spain

    M. José Jara-Palacios, M. Luisa Escudero-Gilete, Belén Gordillo & Francisco J. Heredia

  3. Department of Analytical Chemistry, Facultad de Farmacia, Universidad de Sevilla, 41012, Sevilla, Spain

    Dolores Hernanz

  4. Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato, Irapuato, Guanajuato, Mexico

    Octavio Paredes-López

Authors
  1. Gustavo A. Camelo-Méndez
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  2. M. José Jara-Palacios
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  3. M. Luisa Escudero-Gilete
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  4. Belén Gordillo
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  5. Dolores Hernanz
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  6. Octavio Paredes-López
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  7. Pablo E. Vanegas-Espinoza
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  8. Alma A. Del Villar-Martínez
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  9. Francisco J. Heredia
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Corresponding author

Correspondence to Alma A. Del Villar-Martínez.

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Camelo-Méndez, G.A., Jara-Palacios, M.J., Escudero-Gilete, M.L. et al. Comparative Study of Phenolic Profile, Antioxidant Capacity, and Color-composition Relation of Roselle Cultivars with Contrasting Pigmentation. Plant Foods Hum Nutr 71, 109–114 (2016). https://doi.org/10.1007/s11130-015-0522-5

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  • DOI: https://doi.org/10.1007/s11130-015-0522-5

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