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Phenolic Acids of the Two Major Blueberry Species in the US Market and Their Antioxidant and Anti-inflammatory Activities


Highbush (cultivated) and lowbush (wild) are the two major blueberry species in the US market. Eight phenolic acids were detected and quantified from these two species by HPLC-MS. Chlorogenic acid was found to be the predominant phenolic acid in both species, with 0.44 mg/g fresh weight in lowbush blueberries and 0.13 mg/g fresh weight in highbush blueberries. Total phenolic content in lowbush blueberries is over three times higher than that of highbush blueberries. The phenolic acid mixtures representing those in the two species were prepared by using authentic standards to assess their contribution to total antioxidant and anti-inflammatory activities of the whole berries. Neither lowbush nor highbush blueberry phenolic acid mixture contributed significantly to the total antioxidant capacity of their relevant whole berries measured by oxygen radical absorbance capacity (ORAC). Both phenolic acid mixtures were able to enter the cell and showed in cell antioxidant activities from the cell based antioxidant protection of erythrocytes (CAP-e) assay. Lowbush blueberry phenolic acid mixture was found to show anti-inflammatory activities by inhibiting the nuclear factor-κB (NF-κB) activation and the production of inflammatory cytokines (TNF-α and IL-6) at the high dose.

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  1. 1.

    Ebadi M (2006) Blueberry (Vaccinium). In: Pharmacodynamic basis of herbal medicine. second edition edn. CRC Press Boca Raton, pp 209–211

  2. 2.

    Paredes-Lopez O, Cervantes-Ceja ML, Vigna-Perez M, Hernandez-Perez T (2010) Berries: improving human health and healthy aging, and promoting quality life–a review. Plant Foods Hum Nutr 65(3):299–308. doi:10.1007/s11130-010-0177-1

    Article  CAS  Google Scholar 

  3. 3.

    Szajdek A, Borowska EJ (2008) Bioactive compounds and health-promoting properties of berry fruits: a review. Plant Foods Hum Nutr 63(4):147–156. doi:10.1007/s11130-008-0097-5

    Article  CAS  Google Scholar 

  4. 4.

    Cheng A, Yan H, Han C, Wang W, Tian Y, Chen X (2014) Polyphenols from blueberries modulate inflammation cytokines in LPS-induced RAW264.7 macrophages. Int J Biol Macromol 69:382–387. doi:10.1016/j.ijbiomac.2014.05.071

    Article  CAS  Google Scholar 

  5. 5.

    Wu X, Beecher GR, Holden JM, Haytowitz DB, Gebhardt SE, Prior RL (2004) Lipophilic and hydrophilic antioxidant capacities of common foods in the United States. J Agric Food Chem 52(12):4026–4037. doi:10.1021/jf049696w

    Article  CAS  Google Scholar 

  6. 6.

    Basu A, Du M, Leyva MJ, Sanchez K, Betts NM, Wu M, Aston CE, Lyons TJ (2010) Blueberries decrease cardiovascular risk factors in obese men and women with metabolic syndrome. J Nutr 140(9):1582–1587. doi:10.3945/jn.110.124701

    Article  CAS  Google Scholar 

  7. 7.

    Kay CD, Holub BJ (2002) The effect of wild blueberry (Vaccinium angustifolium) consumption on postprandial serum antioxidant status in human subjects. Br J Nutr 88(4):389–398. doi:10.1079/bjn2002665

    Article  CAS  Google Scholar 

  8. 8.

    Blacker BC, Snyder SM, Eggett DL, Parker TL (2013) Consumption of blueberries with a high-carbohydrate, low-fat breakfast decreases postprandial serum markers of oxidation. Br J Nutr 109(9):1670–1677. doi:10.1017/s0007114512003650

    Article  CAS  Google Scholar 

  9. 9.

    Wu X, Kang J (2011) Blueberries: major phytochemicals and potential health effects in cardiovascular diseases berries: properties, consumption and nutrition. Nova Science Publishers

  10. 10.

    Rodriguez-Mateos A, Cifuentes-Gomez T, Tabatabaee S, Lecras C, Spencer JP (2012) Procyanidin, anthocyanin, and chlorogenic acid contents of highbush and lowbush blueberries. J Agric Food Chem 60(23):5772–5778. doi:10.1021/jf203812w

    Article  CAS  Google Scholar 

  11. 11.

    Manganaris GA, Goulas V, Vicente AR, Terry LA (2014) Berry antioxidants: small fruits providing large benefits. J Sci Food Agric 94(5):825–833. doi:10.1002/jsfa.6432

    Article  CAS  Google Scholar 

  12. 12.

    Pandey KB, Rizvi SI (2009) Plant polyphenols as dietary antioxidants in human health and disease. Oxidative Med Cell Longev 2(5):270–278. doi:10.4161/oxim.2.5.9498

    Article  Google Scholar 

  13. 13.

    Zheng W, Wang SY (2003) Oxygen radical absorbing capacity of phenolics in blueberries, cranberries, chokeberries, and lingonberries. J Agric Food Chem 51(2):502–509. doi:10.1021/jf020728u

    Article  CAS  Google Scholar 

  14. 14.

    Prior RL, Rogers TR, Khanal RC, Wilkes SE, Wu X, Howard LR (2010) Urinary excretion of phenolic acids in rats fed cranberry. J Agric Food Chem 58(7):3940–3949. doi:10.1021/jf9028392

    Article  CAS  Google Scholar 

  15. 15.

    Wu X, Pittman III HE, Hager T, Hager A, Howard L, Prior RL (2009) Phenolic acids in black raspberry and in the gastrointestinal tract of pigs following ingestion of black raspberry. Mol Nutr Food Res 53(Suppl 1):S76–S84. doi:10.1002/mnfr.200800231

    Article  Google Scholar 

  16. 16.

    Chen JR, Lazarenko OP, Wu X, Kang J, Blackburn ML, Shankar K, Badger TM, Ronis MJ (2010) Dietary-induced serum phenolic acids promote bone growth via p38 MAPK/beta-catenin canonical Wnt signaling. J Bone Miner Res 25(11):2399–2411. doi:10.1002/jbmr.137

    Article  CAS  Google Scholar 

  17. 17.

    Xie C, Kang J, Chen JR, Nagarajan S, Badger TM, Wu X (2011) Phenolic acids are in vivo atheroprotective compounds appearing in the serum of rats after blueberry consumption. J Agric Food Chem 59(18):10381–10387. doi:10.1021/jf2025264

    Article  CAS  Google Scholar 

  18. 18.

    Montales MT, Rahal OM, Kang J, Rogers TJ, Prior RL, Wu X, Simmen RC (2012) Repression of mammosphere formation of human breast cancer cells by soy isoflavone genistein and blueberry polyphenolic acids suggests diet-mediated targeting of cancer stem-like/progenitor cells. Carcinogenesis 33(3):652–660. doi:10.1093/carcin/bgr317

    Article  CAS  Google Scholar 

  19. 19.

    Laveti D, Kumar M, Hemalatha R, Sistla R, Naidu VG, Talla V, Verma V, Kaur N, Nagpal R (2013) Anti-inflammatory treatments for chronic diseases: a review. Inflamm Allergy Drug Targets 12(5):349–361

    Article  CAS  Google Scholar 

  20. 20.

    Honzel D, Carter SG, Redman KA, Schauss AG, Endres JR, Jensen GS (2008) Comparison of chemical and cell-based antioxidant methods for evaluation of foods and natural products: generating multifaceted data by parallel testing using erythrocytes and polymorphonuclear cells. J Agric Food Chem 56(18):8319–8325. doi:10.1021/jf800401d

    Article  CAS  Google Scholar 

  21. 21.

    Jensen GS, Wu X, Patterson KM, Barnes J, Carter SG, Scherwitz L, Beaman R, Endres JR, Schauss AG (2008) In vitro and in vivo antioxidant and anti-inflammatory capacities of an antioxidant-rich fruit and berry juice blend. Results of a pilot and randomized, double-blinded, placebo-controlled, crossover study. J Agric Food Chem 56(18):8326–8333. doi:10.1021/jf8016157

    Article  CAS  Google Scholar 

  22. 22.

    Kang J, Thakali KM, Xie C, Kondo M, Tong Y, Ou B, Jensen G, Medina MB, Schauss AG, Wu X (2012) Bioactivities of açaí (Euterpe precatoria Mart.) fruit pulp, superior antioxidant and anti-inflammatory properties to Euterpe oleracea Mart. Food Chem 133(3):671–677. doi:10.1016/j.foodchem.2012.01.048

    Article  CAS  Google Scholar 

  23. 23.

    Zadernowski R, Naczk M, Nesterowicz J (2005) Phenolic acid profiles in some small berries. J Agric Food Chem 53(6):2118–2124. doi:10.1021/jf040411p

    Article  CAS  Google Scholar 

  24. 24.

    Mattila P, Hellstrom J, Torronen R (2006) Phenolic acids in berries, fruits, and beverages. J Agric Food Chem 54(19):7193–7199. doi:10.1021/jf0615247

    Article  CAS  Google Scholar 

  25. 25.

    Scalzo J, Politi A, Pellegrini N, Mezzetti B, Battino M (2005) Plant genotype affects total antioxidant capacity and phenolic contents in fruit. Nutrition 21(2):207–213. doi:10.1016/j.nut.2004.03.025

    Article  CAS  Google Scholar 

  26. 26.

    Kang J, Li Z, Wu T, Jensen GS, Schauss AG, Wu X (2010) Anti-oxidant capacities of flavonoid compounds isolated from acai pulp (Euterpe oleracea Mart.). Food Chem 122(3):610–617. doi:10.1016/j.foodchem.2010.03.020

    Article  CAS  Google Scholar 

  27. 27.

    Niki E (2011) Antioxidant capacity: which capacity and how to assess it? J Berry Res 1(4):169–176. doi:10.3233/jbr-2011-018

    Google Scholar 

  28. 28.

    Prior RL, Wu X, Schaich K (2005) Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem 53(10):4290–4302. doi:10.1021/jf0502698

    Article  CAS  Google Scholar 

  29. 29.

    Prior RL, Gu L, Wu X, Jacob RA, Sotoudeh G, Kader AA, Cook RA (2007) Plasma antioxidant capacity changes following a meal as a measure of the ability of a food to alter in vivo antioxidant status. J Am Coll Nutr 26(2):170–181

    Article  CAS  Google Scholar 

  30. 30.

    Lopez-Alarcon C, Denicola A (2013) Evaluating the antioxidant capacity of natural products: a review on chemical and cellular-based assays. Anal Chim Acta 763:1–10. doi:10.1016/j.aca.2012.11.051

    Article  CAS  Google Scholar 

  31. 31.

    Chiva-Blanch G, Visioli F (2012) Polyphenols and health: moving beyond antioxidants. J Berry Res 2(2):63–71. doi:10.3233/jbr-2012-028

    CAS  Google Scholar 

  32. 32.

    dos Santos MD, Almeida MC, Lopes NP, de Souza GE (2006) Evaluation of the anti-inflammatory, analgesic and antipyretic activities of the natural polyphenol chlorogenic acid. Biol Pharm Bull 29(11):2236–2240

    Article  Google Scholar 

  33. 33.

    Ruifeng G, Yunhe F, Zhengkai W, Ershun Z, Yimeng L, Minjun Y, Xiaojing S, Zhengtao Y, Naisheng Z (2014) Chlorogenic acid attenuates lipopolysaccharide-induced mice mastitis by suppressing TLR4-mediated NF-kappaB signaling pathway. Eur J Pharmacol 729C:54–58. doi:10.1016/j.ejphar.2014.01.015

    Article  Google Scholar 

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Conflict of Interest

The authors declare that there is no conflict of interest.

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Corresponding author

Correspondence to Xianli Wu.

Additional information

This study was supported by USDA (6251-51000-005-02S).

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Kang, J., Thakali, K.M., Jensen, G.S. et al. Phenolic Acids of the Two Major Blueberry Species in the US Market and Their Antioxidant and Anti-inflammatory Activities. Plant Foods Hum Nutr 70, 56–62 (2015).

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  • Anti-inflammation
  • Antioxidant
  • Blueberries
  • Phenolic acid