Abstract
The aim of this study was to investigate the bioactivity of the essential oil isolated from Origanum vulgare L. (EOv). We analyzed the in vivo anti-inflammatory properties in a mouse-airway inflammation model and the in vitro antimicrobial activity, genotoxicity over the anaphase-telophase with the Allium cepa strain and its cytotoxicity/viability in A549 culture cells. In vivo, EOv modified the levels of tumor necrosis factor -α and viable activated macrophages and was capable to mitigate the effects of degradation of conjugated dienes. In vitro, EOv reduced the viability of cultured A549 cells as well as the mitotic index and a number of chromosomal aberrations; however, it did not change the number of phases. We found that EOv presents antimicrobial activity against different Gram (−) and (+) strains, measured by disc-diffusion test and confirmed with a more accurate method, the AutoCad software. We postulate that EOv presents antibacterial, antioxidant and chemopreventive properties and could be play an important role as bioprotector agent.
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Abbreviations
- AMs :
-
Alveolar macrophages
- BALF :
-
Bronchoalveolar lavage fluid
- CDs :
-
Conjugated dienes
- EOs :
-
Essential oils
- EOv :
-
Essential oil of Origanum vulgare
- PMNs :
-
Polymorphonuclear neutrophils
- TNF-α :
-
Tumor necrosis factor-α
References
Shahsavari N, Barzegar M, Sahari MA, Naghdibadi H (2008) Antioxidant activity and chemical characterization of essential oil of Bunium persicum. Plant Foods Hum Nutr 63(4):183–188
Pérez GS, Zavala SM, Arias GL, Ramos LM (2011) Anti-inflammatory activity of some essential oils. J Essent Oil Res 23(5):38–44
Kordali S, Cakir A, Ozer H, Cakmakci R, Kesdek M, Mete E (2008) Antifungal, phytotoxic and insecticidal properties of essential oil isolated from Turkish Origanum acutidens and its three components, carvacrol, thymol and p-cymene. Biores Tech 99(18):8788–8795
Aligiannis N, Kalpoutzakis E, Mitaku S, Chinou IB (2001) Composition and antimicrobial activity of the essential oils of two Origanum species. J Agric Food Chem 49(9):4168–4170
García-Pérez E, Noratto GD, García-Lara S, Gutiérrez-Uribe JA, Mertens-Talcott SU (2013) Micropropagation effect on the anti-carcinogenic activitiy of polyphenolics from Mexican Oregano (Poliomintha glabrescens Gray) in human colon cancer cells HT-29. Plant Foods Hum Nutr 68(2):155–162
Dambolena JS, Zunino MP, Lucini EI, Olmedo R, Banchio E, Bima PJ, Zygadlo JA (2010) Total phenolic content, radical scavenging properties, and essential oil composition of Origanum species from different populations. J Agric Food Chem 58(2):1115–1120
Bigliani MC, Rossetti V, Grondona E, Lo Presti S, Paglini PM, Rivero V, Zunino MP, Ponce AA (2012) Chemical compositions and properties of Schinus areira L. essential oil on airway inflammation and cardiovascular system of mice and rabbits. Food Chem Toxicol 50(7):2282–2288
Roque N, Cremonezzi D, Bigliani CM, Grondona E, Zunino MP, Ponce AA (2009) Biphasic modulation of neutrophil migration by aqueous extracts of Uncaria tomentosa in murine lung. J Compl Int Med 6 (1): ISSN (Online) 1553–3840, DOI: 10.2202/1553-3840.1222
Bigliani MC, Grondona E, Zunino PM, Ponce AA (2010) Effects of Cecropia pachystachya and Larrea divaricata aqueous extracts in mice. Hum Exp Toxicol 29(7):601–606
Ogura H, Cioffi WG, Okerberg CV, Johnson AA, Guzman RF, Mason AD Jr, Pruitt BA Jr (1994) The effects of pentoxifylline on pulmonary function following smoke inhalation. J Surg Res 56(3):242–250
Fiskesjo G (1988) The Allium test, an alternative in environmental studies: the relative toxicity of metal ions. Mutat Res 197(2):243–260
Grant WF (1982) Chromosome aberration assays in Allium. A report of the U.S. environmental protection agency gene-tox program. Mutat Res 99(3):273–291
Valeriano C, de Oliveira TLC, de Carvalho SM, Cardoso MG, Alves E, Piccoli RH (2012) The sanitizing action of essential oil-based solutions against Salmonella enterica serotype Enteritidis S64 biofilm formation on AISI 304 stainless steel. Food Control 25(2):673–677
Bukovska A, Cikos S, Juhas S, Il’kova G, Rehak P, Koppel J (2007) Effects of a combination of thyme and oregano essential oils on TNBS-induced colitis in mice. Mediators Inflamm 2007:23296
Arcila-Lozano CC, Loarca-Piña G, Lecona-Uribe S, González de Mejía E (2004) El orégano: propiedades, composición y actividad biológica de sus componentes. Arch Lat Nutr 54:100–111
Stelter K, Frahm J, Paulsen J, Berk A, Kleinwächter M, Selmar D, Dänicke S (2013) Effects of oregano on performance and immunmodulating factors in weaned piglets. Arch Anim Nutr 67(6):461–476
Koparal AT, Zeytinoglu M (2003) Effects of carvacrol on a human non-small cell lung cancer (NSCLC) cell line, A549. Cytotechnology 43(1–3):149–154
Ocana-Fuentes A, Arranz-Gutiérrez E, Senorans F, Reglero G (2010) Supercritical fluid extraction of oregano (Origanum vulgare) essentials oils: anti-inflammatory properties based on cytokine response on THP-1 macrophages. Food Chem Toxicol 48(6):1568–1575
Dundar E, Olgun EG, Isiksoy S, Kurkcuoglu M, Baser KH, Bal C (2008) The effects of intra-rectal and intra-peritoneal application of Origanum onites L. essential oil on 2,4,6-trinitrobenzenesulfonic acid-induced colitis in the rat. Exp Toxicol Pathol 59(6):399–408
Shen D, Pan M-H, Wu Q-L, Park C-H, Juliani HR, Ho C-T, Simon JE (2010) LC-MS method for the simultaneous quantitation of the anti-inflammatory constituents in oregano (Origanum species). J Agric Food Chem 58(12):7119–7125
Ruberto G, Baratta MT (2000) Antioxidant activity of selected essential oil components in two lipid model systems. Food Chem 69(2):167–174
Nencini C, Menchiari A, Franchi GG, Micheli L (2011) In vitro antioxidant activity of aged extracts of some Italian Allium species. Plant Foods Hum Nutr 66(1):11–16
Botsoglou NA, Christaki E, Fletouris DJ, Florou-Paneri P, Spais AB (2002) The effect of dietary oregano essential oil on lipid oxidation in raw and cooked chicken during refrigerated storage. Meat Sci 62(2):259–265
Till GO, Hatherill JR, Tourtellotte WW, Lutz MJ, Ward PA (1985) Lipid peroxidation and acute lung injury after thermal trauma to skin. Evidence of a role for hydroxyl radical. Am J Pathol 119(3):376–384
Richter C (1987) Biophysical consecuences of lipid peroxidation in membranes. Chem Phys Lipids 44:175–189
Grassmann J (2005) Terpenoids as plant antioxidants. Vitam Horm 72:505–535
Dotan Y, Lichtenberg D, Pinchuk I (2004) Lipid peroxidation cannot be used as a universal criterion of oxidative stress. Prog Lipid Res 43(3):200–227
Shokrzadeh M, Ahmadi A, Chabra A, Naghshvar F, Salehi F, Habibi E, Haghi-Aminjan H (2014) An ethanol extract of Origanum vulgare attenuates cyclophosphamide-induced pulmonary injury and oxidative lung damage in mice. Pharm Biol (0):1–8
Ipek E, Zeytinoglu H, Okay S, Tuylu BA, Kurkcuoglu M, Baser K (2005) Genotoxicity and antigenotoxicity of Origanum oil and carvacrol evaluated by Ames Salmonella/microsomal test. Food Chem 93(3):551–556
Savini I, Arnone R, Catani MV, Avigliano L (2009) Origanum vulgare induces apoptosis in human colon cancer caco2 cells. Nutr Cancer 61(3):381–389
Adeyemo OA, Farinmade AE (2013) Genotoxic and cytotoxic effects of food flavor enhancer, monosodium glutamate (MSG) using Allium cepa assay. Afr J Biotech 12(13):1459–1466
Karkabounas S, Kostoula O, Daskalou T, Veltsistas P, Karamouzis M, Zelovitis I, Metsios A, Lekkas P, Evangelou A, Kotsis N (2006) Anticarcinogenic and antiplatelet effects of carvacrol. Exp Oncol 28(2):121–125
Mezzoug N, Elhadri A, Dallouh A, Amkiss S, Skali N, Abrini J, Zhiri A, Baudoux D, Diallo B, El Jaziri M (2007) Investigation of the mutagenic and antimutagenic effects of Origanum compactum essential oil and some of its constituents. Mut Res/Gen Toxicol Env Mut 629(2):100–110
Ceker S, Nardemir G, Alpsoy L, Agar G, Mete E (2012) Anti-genotoxic and anti-oxidant effects of Origanum rotundifolium on human lymphocytes in vitro. J Ess Oil Bea Plants 15(3):415–423
Zunino MP, Turina AV, Zygadlo JA, Perillo MA (2011) Stereoselective effects of monoterpenes on the microviscosity and curvature of model membranes assessed by DPH steady-state fluorescence anisotropy and light scattering analysis. Chirality 23(10):867–877
Acknowledgments
This research project was performed thanks to the financial support of the Research University Grants Scheme: Universidad Nacional de la Rioja and Universidad Nacional de Córdoba (SECyT), Argentina. Agencia Córdoba Ciencia (N° 000113/2011) Argentina. The authors acknowledge the contribution of Mr. Jeff G. Leid, W.L. Gore and Associates for revising the English language.
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could constitute as a potential conflict of interest.
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Grondona, E., Gatti, G., López, A.G. et al. Bio-efficacy of the Essential Oil of Oregano (Origanum vulgare Lamiaceae. Ssp. Hirtum). Plant Foods Hum Nutr 69, 351–357 (2014). https://doi.org/10.1007/s11130-014-0441-x
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DOI: https://doi.org/10.1007/s11130-014-0441-x