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Assessment on Proximate Composition, Dietary Fiber, Phytic Acid and Protein Hydrolysis of Germinated Ecuatorian Brown Rice

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Abstract

Germinated brown rice (GBR) is considered healthier than brown rice (BR) but its nutritive value has been hardly studied. Since nutritive quality of GBR depends on genetic diversity and germination conditions, six Ecuadorian BR varieties were germinated at 28 and 34 ºC for 48 and 96 h in darkness and proximate composition, dietary fiber fractions, phytic acid content as well as degree of protein hydrolysis and peptide content were studied. Protein, lipids, ash and available carbohydrate ranged 7.3–10.4 %, 2.0–4.0 %, 0.8–1.5 % and 71.6 to 84.0 %, respectively, in GBR seedlings. Total dietary fiber increased during germination (6.1–13.6 %), with a large proportion of insoluble fraction, while phytic acid was reduced noticeably. In general, protein hydrolysis occurred during germination was more accused at 28 ºC for 48 h. These results suggest that GBR can be consumed directly as nutritive staple food for a large population worldwide contributing to their nutritional requirements.

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References

  1. OECD-FAO Agricultural Outlook 2013–2022 bchsooiaqDeoAUGfOS

  2. http://agrytec.com/agricola/index.php?option=com_content&view=article&id=7296:magappuso-a-prueba calidad-semillas-de-arroz-y-maiz&catid = 18:noticias&Itemid = 57

  3. Sharif MK, Butt MS, Anjum FM, Khan SH (2014) Rice bran: a novel functional ingredient. Crit Rev Food Sci Nutr 54:807–816

    Article  CAS  Google Scholar 

  4. Wu F, Chen H, Yang N, Duan X, Jin Z, Xu X (2013) Germinated brown rice enhances antioxidant activities and immune functions in aged mice. Cereal Chem 90(6):601–607

    Article  CAS  Google Scholar 

  5. Wang SY, Chen CW, Lin YT, Chang CY (2012) Inhibitory effect on human leukemic U937 cell growth and immunomodulatory activity of germinated brown rice. J Food Drug Anal 20:699–704+717. doi:10.6227/jfda.2012200319

    Google Scholar 

  6. Park DK, Park HJ (2013) Ethanol extract of antrodia camphorata grown on germinated brown rice suppresses inflammatory responses in mice with acute DSS-induced colitis. Evid Based Complement Alternat Med. doi:10.1155/2013/914524, Article number 914524

    Google Scholar 

  7. Ho JN, Son ME, Lim WC, Lim ST, Cho HY (2013) Germinated brown rice extract inhibits adipogenesis through the down-regulation of adipogenic genes in 3 T3-L1 adipocytes. Plant Foods Hum Nutr 68:274–278

  8. Azmi NH, Ismail N, Imam MU, Ismail M (2013) Ethyl acetate extract of germinated brown rice attenuates hydrogen peroxide-induced oxidative stress in human SH-SY5Y neuroblastoma cells: role of anti-apoptotic, pro-survival and antioxidant genes. BMC Complement Altern Med 13:177–188

    Article  Google Scholar 

  9. Dogra V, Ahuja PS, Sreenivasulu Y (2013) Change in protein content during seed germination of a high altitude plant Podophyllum hexandrum Royle. J Proteomics 78:26–38

    Article  CAS  Google Scholar 

  10. Xu J, Zhang H, Guo X, Qian H (2012) The impact of germination on the characteristics of brown rice flour and starch. J Sci Food Agric 92:380–387

    Article  CAS  Google Scholar 

  11. El-Adawy TA, Rahma EH, El-Bedawey AA, El-Beltagy AE (2003) Nutritional potential and functional properties of germinated mung bean, pea and lentil seeds. Plant Foods Hum Nutr 58:1–13

    Article  Google Scholar 

  12. Chang YP, Tan MP, Lok WL, Pakianathan S, Supramaniam Y (2014) Making use of guava seed (Psidium guajava L): the effects of pre-treatments on its chemical composition. Plant Foods Hum Nutr 69:43–49

    Article  CAS  Google Scholar 

  13. Albarracín M, González RJ, Drago SR (2013) Effect of soaking process on nutrient bio-accessibility and phytic acid content of brown rice cultivar. LWT-Food Sci Technol 53:76–80. doi:10.1016/j.lwt.2013.01.029

    Article  Google Scholar 

  14. Moongngarm A, Saetung N (2010) Comparison of chemical compositions and bioactive compounds of germinated rough rice and brown rice. Food Chem 122:782–788

    Article  CAS  Google Scholar 

  15. Koehler P, Hartmann G, Wieser H, Rychlik M (2007) Changes of folates, dietary fiber, and proteins in wheat as affected by germination. J Agric Food Chem 55:4678–4683

    Article  CAS  Google Scholar 

  16. Seki T, Nagase R, Torimitsu M, Yanagi M, Ito Y, Kise M, Mizukuchi A, Fujimura N, Hayamizu K, Ariga T (2005) Insoluble fiber is a major constituent responsible for lowering the post-prandial blood glucose concentration in the pre-germinated brown rice. Biol Pharml Bull 28:1539–1541

    Article  CAS  Google Scholar 

  17. Cáceres PJ, Martínez-Villaluenga C, Amigo L, Frias J (2014) Maximising the phytochemical content and antioxidant activity of Ecuadorian brown rice sprouts through optimal germination conditions. Food Chem 152:407–414

    Article  Google Scholar 

  18. AOAC (1990) Official Methods of Analysis of the Association of Official Analytical Chemists. 15th edition. Edited by Kenneth Helrich. Virginia 22201, USA

  19. AOAC (2000) Official Methods of Analysis of the Association of Official Analytical Chemists. 17th edition. Edited by William Horwitz. Maryland 20877–2417. USA

  20. FAO (2002) Food energy – methods of analysis and conversion factors. Food and Nutrition. Paper 77. FAO Rome

  21. Reichwald K, Hatzack F (2008) Application of a modified Haug and Lantzsch method for the rapid and accurate photometrical phytate determination in soybean, wheat, and maize meals. J Agric Food Chem 56:2888–2891

    Article  CAS  Google Scholar 

  22. Torino MI, Limon RI, Martinez-Villaluenga C, Makinen S, Pihlanto A, Vidal-Valverde C, Frias J (2013) Antioxidant and antihypertensive properties of liquid and solid state fermented lentils. Food Chem 136:1030–1037. doi:10.1016/j.foodchem.2012.09.015

    Article  CAS  Google Scholar 

  23. Megat Rusydi MR, Noraliza CW, Azrina A, Zulkhairi A (2011) Nutritional changes in germinated legumes and rice varieties. Int Food Res J 18(2):705–713. http://www.ifrj.upm.edu.my/18%20(02)%202011/(33)%20IFRJ-2010-145.pdf

    Google Scholar 

  24. Veluppillai S, Nithyanantharajah K, Vasantharuba S, Balakumar S, Arasaratnam V (2009) Biochemical changes associated with germinating rice grains and germination improvement. Rice Sci 16:240–242

    Article  Google Scholar 

  25. Hsu TF, Kise M, Wang MF, Ito Y, Yang MD, Aoto H, Yoshihara R, Yokoyama J, Kunii D, Yamamoto S (2008) Effects of pre-germinated brown rice on blood glucose and lipid levels in free- living patients with impaired fasting glucose or type 2 diabetes. J Nutr Sci Vitaminol 54:163–168

    Article  CAS  Google Scholar 

  26. Kim HY, Hwang IG, Kim TM, Woo KS, Park DS, Kim JH, Kim DJ, Lee J, Lee YR, Jeong HS (2012) Chemical and functional components in different parts of rough rice (Oryza sativa L.) before and after germination. Food Chem 134:288–293

    Article  CAS  Google Scholar 

  27. Hahm TS, Park SJ, Martin Lo Y (2009) Effects of germination on chemical composition and functional properties of sesame (Sesamum indicum L.) seeds. Bioresource Technol 100:1643–1647

    Article  CAS  Google Scholar 

  28. Raghuram TC, Rukmini C (1995) Nutritional significance of rice bran oil. Indian J Med Res 102:241–244

    CAS  Google Scholar 

  29. Lin PY, Lai HM (2011) Bioactive compounds in rice during grain development. Food Chem 127:86–93

    Article  CAS  Google Scholar 

  30. Martín-Cabrejas MA, Díaz MF, Aguilera Y, Benítez V, Mollá E, Esteban RM (2008) Influence of germination on the soluble carbohydrates and dietary fibre fractions in non-conventional legumes. Food Chem 107:1045–1052

    Article  Google Scholar 

  31. Ohtsubo K, Suzuki K, Yasui Y, Kasumi T (2005) Bio-functional components in the processed pre-germinated brown rice by a twin-screw extruder. J Food Compos Anal 18:303–316

    Article  CAS  Google Scholar 

  32. Liang J, Han BZ, Nout MJR, Hamer RJ (2008) Effects of soaking, germination and fermentation on phytic acid, total and in vitro soluble zinc in brown rice. Food Chem 110:821–828

    Article  CAS  Google Scholar 

  33. Kumar V, Sinha AK, Makkar HPS, Becker K (2010) Dietary roles of phytate and phytase in human nutrition: a review. Food Chem 120:945–959

    Article  CAS  Google Scholar 

  34. Limón RI, Peñas E, Martínez-Villaluenga C, Frias J (2014) Role of elicitation on the health- promoting properties of kidney bean sprouts. LWT-Food Sci Technol 56:328–334. doi:10.1016/j.lwt.2013.12.014

    Article  Google Scholar 

  35. Dei Piu L, Tassoni A, Serrazanetti DI, Ferri M, Babini E, Tagliazucchi D, Gianotti A (2014) Exploitation of starch industry liquid by-product to produce bioactive peptides from rice hydrolyzed proteins. Food Chem 155:199–206

    Article  CAS  Google Scholar 

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Acknowledgments

P.J.C. is indebted to the Ecuadorian government through the SENESCYT (Secretaría Nacional de Educación Superior, Ciencia, Tecnología e Innovación, Ecuador) support on his doctoral studies. We also acknowledge to INIAP (Instituto Nacional de Investigaciones Agropecuarias of Ecuador) and to INDIA-PRONACA (Productora Nacional de Alimentos C.A.) for providing the BR seeds. We are grateful to M.F. Romero and A. Galarza for their technical assistance. This work was partly funded by AGL2010-16310 Project from the Ministry of Economy and Competitiveness (MINECO, Spain).

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Correspondence to Juana Frias.

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Cáceres, P.J., Martínez-Villaluenga, C., Amigo, L. et al. Assessment on Proximate Composition, Dietary Fiber, Phytic Acid and Protein Hydrolysis of Germinated Ecuatorian Brown Rice. Plant Foods Hum Nutr 69, 261–267 (2014). https://doi.org/10.1007/s11130-014-0433-x

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