Plant Foods for Human Nutrition

, Volume 67, Issue 4, pp 422–429 | Cite as

Cytotoxic Effect of Natural trans-Resveratrol Obtained from Elicited Vitis vinifera Cell Cultures on Three Cancer Cell Lines

  • Francisco Fernández-Pérez
  • Sarai Belchí-Navarro
  • Lorena Almagro
  • Roque Bru
  • Maria A. Pedreño
  • Laura V. Gómez-Ros
Original Paper

Abstract

trans-Resveratrol (trans-R) has been reported to be a potential cancer chemopreventive agent. Although its cytotoxic activity against different cancer cell lines has been tested, its effect on human acute leukemia cell lines has scarcely been investigated, and only a few in vitro studies were performed using human breast epithelial cell lines. Due to its potential value for human health, demand for trans-R has rapidly increased, and new biotechnological strategies to obtain it from natural edible sources have been developed. Thus, grapevine cell cultures represent a reliable system of trans-R production since they biosynthesize trans-R constitutively or in response to elicitation. In addition, there are no studies deepen on the inhibitory effect of trans-R, produced by elicited grapevine cell cultures, on growth of human tumor cell lines. In this work, the effect of trans-R extracted from the culture medium, after elicitation of grapevine cell cultures, was tested on two human acute lymphocytic and monocytic leukemia cell lines, and one human breast cancer cell line. The effect of trans-R on cell proliferation was not only dose- and time-dependent but also cell type-dependent, as seen from the different degrees of susceptibility of cancer cell lines tested. As regards the effect of trans-R on cell cycle distribution, low trans-R concentrations increased cells in the S phase whereas a high trans-R concentration increased G0/G1 phase in all cell lines. Perturbation of the cell cycle at low trans-R concentrations did not correlate with the induction of cell death, whereas a high trans-R concentration, cell proliferation decreased as a result of increasing apoptosis in the three cell lines. In leukemia cells, trans-R up-regulated the expression of caspase-3 while trans-R-induced apoptosis in breast cells occur through a caspase-3-independent mechanism mediated by a down-regulation of Bcl-2.

Keywords

Antitumor Grapevine cell cultures Elicitation trans-Resveratrol Human cancer cell lines 

Abbreviations

CDs

Cyclodextrins

IC50

Median inhibitory concentration

trans-R

trans-Resveratrol

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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Francisco Fernández-Pérez
    • 1
  • Sarai Belchí-Navarro
    • 1
  • Lorena Almagro
    • 1
  • Roque Bru
    • 2
  • Maria A. Pedreño
    • 1
  • Laura V. Gómez-Ros
    • 1
  1. 1.Department of Plant Biology, Faculty of BiologyUniversity of Murcia, Campus Universitario de EspinardoMurciaSpain
  2. 2.Department of Agrochemistry and Biochemistry, Faculty of SciencesUniversity of Alicante, Campus Universitario de San Vicente de RaspeigAlicanteSpain

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