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Comparative Study of Lipophilic and Hydrophilic Antioxidants from In vivo and In vitro Grown Coriandrum sativum

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Abstract

Coriander is commonly used for medicinal purposes, food applications, cosmetics and perfumes. Herein, the production of antioxidants in vegetative parts (leaves and stems) of in vivo and in vitro grown samples was compared. In vitro samples were clone A- with notorious purple pigmentation in stems and leaves and clone B- green. Seeds were also studied as they are used to obtain in vivo and in vitro vegetative parts. Lipophilic (tocopherols, carotenoids and chlorophylls) and hydrophilic (sugars, ascorbic acid, phenolics, flavonols and anthocyanins) compounds were quantified. The antioxidant activity was evaluated by radical scavenging activity, reducing power and lipid peroxidation inhibition. The in vivo sample showed the highest antioxidant activity mainly due to its highest levels of hydrophilic compounds. Otherwise, in vitro samples, mainly clone A, gave the highest concentration in lipophilic compounds but a different profile when compared to the in vivo sample. Clones A and B revealed a lack of β-carotene, β- and δ-tocopherols, a decrease in α-tocopherol, and an increase in γ-tocopherol and clorophylls in comparison to the in vivo sample. In vitro culture might be useful to explore the plants potentialities for industrial applications, controlling environmental conditions to produce higher amounts of some bioactive products.

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Abbreviations

CAE:

Chlorogenic acid equivalents

DPPH:

2,2-Diphenyl-1-picrylhydrazyl

HPLC:

High-performance liquid chromatography

IS:

Internal Standard

ME:

Malvidin 3-glucoside equivalents

MS:

Mass Spectrometry

QE:

Quercetin equivalents

RI:

Refraction Index

TBARS:

Thiobarbituric acid reactive substances

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Acknowledgements

The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support to the research centre CIMO. L. Barros also thanks to FCT, POPH-QREN and FSE for her grant (SFRH/BPD/4609/2008).

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Correspondence to Isabel C. F. R. Ferreira.

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Dias, M.I., Barros, L., Sousa, M.J. et al. Comparative Study of Lipophilic and Hydrophilic Antioxidants from In vivo and In vitro Grown Coriandrum sativum . Plant Foods Hum Nutr 66, 181–186 (2011). https://doi.org/10.1007/s11130-011-0227-3

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  • DOI: https://doi.org/10.1007/s11130-011-0227-3

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