Plant Foods for Human Nutrition

, Volume 66, Issue 2, pp 129–135 | Cite as

Antigenotoxicity and Antioxidant Activity of Acerola Fruit (Malpighia glabra L.) at Two Stages of Ripeness

  • Roberta da Silva Nunes
  • Vivian Francília Silva Kahl
  • Merielen da Silva Sarmento
  • Marc François Richter
  • Letícia Veras Costa-Lotufo
  • Felipe Augusto Rocha Rodrigues
  • Juan Andres Abin-Carriquiry
  • Marcela María Martinez
  • Scharline Ferronatto
  • Alexandre de Barros Falcão Ferraz
  • Juliana da Silva
ORIGINAL PAPER

Abstract

Genotoxic and antigenotoxic effects of acerola fruit at two stages of ripeness were investigated using mice blood cells. The results show that no ripeness stage of acerola extracts presented any genotoxic potential to damage DNA (Comet assay) or cytotoxicity (MTT assay). When antigenotoxic activity was analyzed, unripe fruit presented higher DNA protection than ripe fruit (red color) extract. The antioxidant capacity of substances also showed that unripe samples inhibit the free radical DPPH more significantly than the ripe ones. The results about determination of compounds made using HPLC showed that unripe acerola presents higher levels of vitamin C as compared to ripe acerola. Thus, vitamin C and the complex mixture of nutrients of Malpighia glabra L., and especially its ripeness stages, influenced the interaction of the fruit extract with the DNA. Acerola is usually consumed when ripe (red fruit), although it is the green fruit (unripe) that has higher potential as beneficial to DNA, protecting it against oxidative stress.

Keywords

Malpighia glabra L. Acerola Genotoxicity Antigenotoxicity Vitamin C 

Abbreviations

AEAC

Ascorbic acid equivalent antioxidant capacity

AOA

Antioxidant activity

DF

Damage frequency

DI

Damage index

DNA

Deoxyribonucleic acid

DMSO

Dimethyl sulfoxide

DOX

Doxorubicin

DPPH

2,2-diphenyl-1-picrylhydrazyl radical

EDTA

Ethylenediaminetetraacetic acid

HCl

Hydrochloric acid

HCT-8

Tumor line of human colon

H2O2

Hydrogen peroxide

HPLC

High performance liquid chromatography

H3PO4

Phosphoric acid

NaCl

Sodium chloride

NaOH

Sodium hydroxide

MDAMDB-435

Tumor line of human breast

MeOH

Methanol

MTT

3-(4,5-dimethyl-2-thiazole)-2,5-diphenyl-2-H-tetrazolium bromide salt

PBS

Phosphate buffered saline

RDA

Recommended Dietary Allowance

SF-295

Tumor line of human nervous system

Tris

Tris(hydroxymethyl)aminomethane

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Roberta da Silva Nunes
    • 1
    • 2
  • Vivian Francília Silva Kahl
    • 1
  • Merielen da Silva Sarmento
    • 1
  • Marc François Richter
    • 3
  • Letícia Veras Costa-Lotufo
    • 4
  • Felipe Augusto Rocha Rodrigues
    • 4
  • Juan Andres Abin-Carriquiry
    • 5
  • Marcela María Martinez
    • 5
  • Scharline Ferronatto
    • 6
  • Alexandre de Barros Falcão Ferraz
    • 6
  • Juliana da Silva
    • 1
  1. 1.Laboratório de Genética Toxicológica—Programa de Pós-Graduação em Genética e Toxicologia AplicadaUniversidade Luterana do BrasilCanoasBrazil
  2. 2.Centro Universitário Luterano de Ji-ParanáUniversidade Luterana do BrasilJi-ParanáBrazil
  3. 3.Universidade Estadual do Rio Grande do Sul, UERGSPorto AlegreBrazil
  4. 4.Laboratório de Oncologia ExperimentalUniversidade Federal do Ceará, UFCFortalezaBrazil
  5. 5.Department of NeurochemistryInstituto de Investigaciones Biológicas Clemente EstableMontevideoUruguay
  6. 6.Laboratório de Farmacognosia e FitoquímicaUniversidade Luterana do BrasilCanoasBrazil

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