Plant Foods for Human Nutrition

, Volume 66, Issue 2, pp 114–121 | Cite as

Organic and Conventional Coffea arabica L.: A Comparative Study of the Chemical Composition and Physiological, Biochemical and Toxicological Effects in Wistar Rats

  • Dayene do Carmo Carvalho
  • Maisa Ribeiro Pereira Lima Brigagão
  • Marcelo Henrique dos Santos
  • Fernanda Borges Araújo de Paula
  • Alexandre Giusti-Paiva
  • Luciana Azevedo


Differentiation between organic and conventional coffee has increased due to the growing demand and high consumption of healthy foods that contain compounds with antioxidant potential, which have been associated with the reduction of chronic diseases. We used organic and conventional coffee in powder 4% (w/w) and infusions 5%, 10% and 20% (w/v) incorporated in a commercial diet to test in vivo. The levels of chlorogenic acid, caffeine and trigonelline were determined by high performance liquid chromatography. The body weight, weight gain, food consumption, aberrant foci crypt, mucin depleted foci, stress biomarkers protein carbonyl and malondialdehyde, biochemical parameters and behavior of the rats were compared between the experimental and control groups within a framework of colon carcinogenesis. The organic coffee showed higher levels of chlorogenic acid, caffeine and trigonelline than conventional, however, this difference did not significantly affect behavior. The infusions had an antioxidant effect, reducing the levels of malondialdehyde; however, the biochemical parameters of the serum were not altered, and there was neither induction nor prevention of preneoplasic lesions.


Antioxidant Coffea arabica L. Colon carcinogenesis Conventional Open-field Organic 



aberrant crypt


aberrant foci crypt


alanine aminotransferase


analysis of variance


Association of Official Analytical Chemists


aspartate aminotransferase


body weight, CCR: conventional coffee diet


commercial diet


organic coffee diet






ethylenediaminetetraacetic acid


Fundação de Apoio á Cultura, Ensino, Pesquisa e Extensão de Alfenas


Fundação de Amparo a Pesquisa de Minas Gerais


high performance liquid chromatography


Laboratório de Análises Nutricional e Toxicológica in vivo




mucin depleted foci, PCO: protein carbonyl



This study was supported by FAPEMIG, Associação de Pequenos Produtores de Poço Fundo-MG, LANTin and FACEPE. Dayene C. Carvalho was recipient of a fellowship of FAPEMIG.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Dayene do Carmo Carvalho
    • 2
  • Maisa Ribeiro Pereira Lima Brigagão
    • 2
  • Marcelo Henrique dos Santos
    • 2
  • Fernanda Borges Araújo de Paula
    • 3
  • Alexandre Giusti-Paiva
    • 4
  • Luciana Azevedo
    • 1
  1. 1.Faculty of NutritionFederal University of Alfenas-MGAlfenasBrazil
  2. 2.Department of Exact ScienceFederal University of Alfenas-MGAlfenasBrazil
  3. 3.Department of Clinical Analysis and ToxicologyFederal University of Alfenas-MGAlfenasBrazil
  4. 4.Department of Biomedical ScienceFederal University of Alfenas-MGAlfenasBrazil

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