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White Tea (Camellia sinensis Kuntze) Exerts Neuroprotection against Hydrogen Peroxide-Induced Toxicity in PC12 Cells

Abstract

Tea is a popular beverage whose consumption is associated with prevention of certain disorders. The objective of the study was to investigate the potential neuroprotective effect of white tea extract (WTE) on hydrogen peroxide induced toxicity in PC12 cells. Cells were treated with various doses of WTE (10–250 μg/ml) before exposition to 250 μM hydrogen peroxide and cell survival was determined through the MTT and LDH assays. Oxidative stress was quantified in the cells after treatments as intracellular reactive oxygen species (ROS) production and the antioxidant activity of the extract was assessed in a cell free system in terms of free radical scavenging capacity. Results showed that WTE has a significant protective effect in the PC12 cell line against hydrogen peroxide as cell survival was significantly superior in WTE-treated cells compared to hydrogen peroxide-treated cells. A reduction on intracellular oxidative stress as well as radical scavenging properties were produced by WTE. Results suggest that WTE protects PC12 cells against H2O2-induced toxicity, and that an antioxidant mechanism through ROS scavenging may be in part responsible for cells neuroprotection.

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References

  1. Sharangi AB (2009) Medicinal and therapeutic potentialities of tea (Camellia sinensis L.)-a review. Food Res Int 42:529–535

    Article  CAS  Google Scholar 

  2. Dufresne CJ, Farnworth ER (2001) A review of latest research findings on the health promotion properties of tea. J Nutr Biochem 12:404–421

    Article  CAS  Google Scholar 

  3. de Mejia EG, Ramirez-Mares MV, Puangpraphant S (2009) Bioactive components of tea: cancer, inflammation and behavior. Brain Behav Immun 23:721–731

    Article  Google Scholar 

  4. Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63

    Article  CAS  Google Scholar 

  5. López V, Martín S, Gómez-Serranillos MP, Carretero ME, Jäger AK, Calvo MI (2009) Neuroprotective and neurological properties of Melissa officinalis. Neurochem Res 34:1955–1961

    Article  Google Scholar 

  6. López V, Akerreta S, Casanova E, García-Mina JM, Cavero RY, Calvo MI (2007) In vitro antioxidant and anti-rhizopus activities of Lamiaceae herbal extracts. Plant Foods Hum Nutr 62:151–155

    Article  Google Scholar 

  7. Song WO, Chun OK (2008) Tea is the major source of flavan-3-ol and flavonol in the U.S. diet. J Nutr 138:1543S–1547S

    CAS  Google Scholar 

  8. Chen Q, Guo Z, Zhao J (2008) Identification of green tea’s (Camellia sinensis (L.)) quality level according to measurement of main catechins and caffeine contents by HPLC and support vector classification pattern recognition. J Pharm Biomed Anal 48:1321–1325

    Article  CAS  Google Scholar 

  9. Clement Y (2009) Can green tea do that? A literature review of the clinical evidence. Prev Med 49:83–87

    Article  Google Scholar 

  10. Coyle CH, Philips BJ, Morrisroe SN, Chancellor MB, Yoshimura N (2008) Antioxidant effects of green tea and its polyphenols on bladder cells. Life Sci 83:12–18

    Article  CAS  Google Scholar 

  11. Gupta S, Saha B, Giri AK (2002) Comparative antimutagenic and anticlastogenic effects of green tea and black tea: a review. Mutat Res 512:37–65

    Article  CAS  Google Scholar 

  12. Kim HJ, Jeon SM, Lee MK, Jung UJ, Shin SK, Choi MS (2009) Antilipogenic effect of green tea extract in C57BL/6J-Lep ob/ob mice. Phytother Res 23:467–471

    Article  CAS  Google Scholar 

  13. Reto M, Figueira ME, Filipe HM, Almeidae CM (2007) Chemical composition of green tea (Camellia sinensis) infusions commercialized in Portugal. Plant Foods Hum Nutr 62:139–144

    Article  CAS  Google Scholar 

  14. Koutelidakis AE, Argiri K, Serafini M, Proestos C, Komaitis M, Pecorari M, Kapsokefalou M (2009) Green tea, white tea, and Pelargonium purpureum increase the antioxidant capacity of plasma and some organs in mice. Nutrition 25:453–458

    Article  CAS  Google Scholar 

  15. Söhle J, Knott A, Holtzmann U, Siegner R, Grönniger E, Schepky A, Gallinat S, Wenck H, Stäb F, Winnefeld M (2009) White tea extract induces lipolytic activity and inhibits adipogenesis in human subcutaneous (pre)- adipocytes. Nutr Metab (Lond) 6:20

    Article  Google Scholar 

  16. Greene LA, Tischler AS (1976) Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc Natl Acad Sci USA 73:2424–2428

    Article  CAS  Google Scholar 

  17. Bruneton J (1999) Pharmacognosy, Phytochemistry, Medicinal Plants, 2nd edn. Tec & Doc, Paris

    Google Scholar 

  18. Weinreb O, Amit T, Mandel S, Youdim MBH (2009) Neuroprotective molecular mechanisms of (-)-epigallocatechin-3-gallate: a reflective outcome of its antioxidant, iron chelating and neuritogenic properties. Genes Nutr 4:283–296

    Article  CAS  Google Scholar 

  19. Cho HS, Kim S, Lee SY, Park JA, Kim SJ, Chun HS (2008) Protective effect of the green tea component, L-theanine on environmental toxins-induced neuronal cell death. Neurotoxicology 29:656–662

    Article  CAS  Google Scholar 

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Acknowledgements

University of Navarra Foundation and Association of Friends of the University of Navarra are thanked for financial support. Dr. M.P. Gómez-Serranillos (Complutense University of Madrid) is thanked for providing PC12 cells.

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Correspondence to Víctor López.

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López, V., Calvo, M.I. White Tea (Camellia sinensis Kuntze) Exerts Neuroprotection against Hydrogen Peroxide-Induced Toxicity in PC12 Cells. Plant Foods Hum Nutr 66, 22–26 (2011). https://doi.org/10.1007/s11130-010-0203-3

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  • DOI: https://doi.org/10.1007/s11130-010-0203-3

Keywords

  • Tea
  • Camellia sinensis
  • PC12 cells
  • Antioxidant
  • Neuroprotection
  • Medicinal plants