Tea is a popular beverage whose consumption is associated with prevention of certain disorders. The objective of the study was to investigate the potential neuroprotective effect of white tea extract (WTE) on hydrogen peroxide induced toxicity in PC12 cells. Cells were treated with various doses of WTE (10–250 μg/ml) before exposition to 250 μM hydrogen peroxide and cell survival was determined through the MTT and LDH assays. Oxidative stress was quantified in the cells after treatments as intracellular reactive oxygen species (ROS) production and the antioxidant activity of the extract was assessed in a cell free system in terms of free radical scavenging capacity. Results showed that WTE has a significant protective effect in the PC12 cell line against hydrogen peroxide as cell survival was significantly superior in WTE-treated cells compared to hydrogen peroxide-treated cells. A reduction on intracellular oxidative stress as well as radical scavenging properties were produced by WTE. Results suggest that WTE protects PC12 cells against H2O2-induced toxicity, and that an antioxidant mechanism through ROS scavenging may be in part responsible for cells neuroprotection.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Price excludes VAT (USA)
Tax calculation will be finalised during checkout.
Sharangi AB (2009) Medicinal and therapeutic potentialities of tea (Camellia sinensis L.)-a review. Food Res Int 42:529–535
Dufresne CJ, Farnworth ER (2001) A review of latest research findings on the health promotion properties of tea. J Nutr Biochem 12:404–421
de Mejia EG, Ramirez-Mares MV, Puangpraphant S (2009) Bioactive components of tea: cancer, inflammation and behavior. Brain Behav Immun 23:721–731
Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63
López V, Martín S, Gómez-Serranillos MP, Carretero ME, Jäger AK, Calvo MI (2009) Neuroprotective and neurological properties of Melissa officinalis. Neurochem Res 34:1955–1961
López V, Akerreta S, Casanova E, García-Mina JM, Cavero RY, Calvo MI (2007) In vitro antioxidant and anti-rhizopus activities of Lamiaceae herbal extracts. Plant Foods Hum Nutr 62:151–155
Song WO, Chun OK (2008) Tea is the major source of flavan-3-ol and flavonol in the U.S. diet. J Nutr 138:1543S–1547S
Chen Q, Guo Z, Zhao J (2008) Identification of green tea’s (Camellia sinensis (L.)) quality level according to measurement of main catechins and caffeine contents by HPLC and support vector classification pattern recognition. J Pharm Biomed Anal 48:1321–1325
Clement Y (2009) Can green tea do that? A literature review of the clinical evidence. Prev Med 49:83–87
Coyle CH, Philips BJ, Morrisroe SN, Chancellor MB, Yoshimura N (2008) Antioxidant effects of green tea and its polyphenols on bladder cells. Life Sci 83:12–18
Gupta S, Saha B, Giri AK (2002) Comparative antimutagenic and anticlastogenic effects of green tea and black tea: a review. Mutat Res 512:37–65
Kim HJ, Jeon SM, Lee MK, Jung UJ, Shin SK, Choi MS (2009) Antilipogenic effect of green tea extract in C57BL/6J-Lep ob/ob mice. Phytother Res 23:467–471
Reto M, Figueira ME, Filipe HM, Almeidae CM (2007) Chemical composition of green tea (Camellia sinensis) infusions commercialized in Portugal. Plant Foods Hum Nutr 62:139–144
Koutelidakis AE, Argiri K, Serafini M, Proestos C, Komaitis M, Pecorari M, Kapsokefalou M (2009) Green tea, white tea, and Pelargonium purpureum increase the antioxidant capacity of plasma and some organs in mice. Nutrition 25:453–458
Söhle J, Knott A, Holtzmann U, Siegner R, Grönniger E, Schepky A, Gallinat S, Wenck H, Stäb F, Winnefeld M (2009) White tea extract induces lipolytic activity and inhibits adipogenesis in human subcutaneous (pre)- adipocytes. Nutr Metab (Lond) 6:20
Greene LA, Tischler AS (1976) Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc Natl Acad Sci USA 73:2424–2428
Bruneton J (1999) Pharmacognosy, Phytochemistry, Medicinal Plants, 2nd edn. Tec & Doc, Paris
Weinreb O, Amit T, Mandel S, Youdim MBH (2009) Neuroprotective molecular mechanisms of (-)-epigallocatechin-3-gallate: a reflective outcome of its antioxidant, iron chelating and neuritogenic properties. Genes Nutr 4:283–296
Cho HS, Kim S, Lee SY, Park JA, Kim SJ, Chun HS (2008) Protective effect of the green tea component, L-theanine on environmental toxins-induced neuronal cell death. Neurotoxicology 29:656–662
University of Navarra Foundation and Association of Friends of the University of Navarra are thanked for financial support. Dr. M.P. Gómez-Serranillos (Complutense University of Madrid) is thanked for providing PC12 cells.
About this article
Cite this article
López, V., Calvo, M.I. White Tea (Camellia sinensis Kuntze) Exerts Neuroprotection against Hydrogen Peroxide-Induced Toxicity in PC12 Cells. Plant Foods Hum Nutr 66, 22–26 (2011). https://doi.org/10.1007/s11130-010-0203-3