Plant Foods for Human Nutrition

, Volume 65, Issue 3, pp 225–232 | Cite as

Screening of Dried Plant Seed Extracts for Adiponectin Production Activity and Tumor Necrosis Factor-Alpha Inhibitory Activity on 3T3-L1 Adipocytes

  • Yoshinori OkadaEmail author
  • Mizue Okada
  • Yumi Sagesaka


To search for dried plant seeds with potent anti-diabetes activity, we conducted a large scale screening for inhibitory activity on tumor necrosis factor-alpha and facilitating activity on adiponectin production in vitro. These activities in 3T3-L1 adipocytes were screened from ethanol extracts of 20 kinds of dried plant seed marketed in Japan. komatsuna (Brassica rapa var. perviridis), common bean (Phaseolus vulgaris L.), qing geng cai (Brassica rapa var. chinensis), green soybean (Glycine max), spinach (Spinacia oleracea L.) and sugar snap pea (Pisum sativum L.) markedly enhanced adiponectin production (11.3 ~ 12.7 ng/ml) but Japanese radish (Raphanus sativus), edible burdock (Arctium lappa L.), bitter melon (Momordica charantia) and broccoli (Brassica oleracea var. italica) did not (0.9 ~ 2.7 ng/ml). All adiponectin-production-enhancing seeds except spinach (2.7 pg/ml) and okra (Abelmoschus esculentus) (6.6 pg/ml) effectively decreased tumor necrosis factor-alpha levels (0.0 pg/ml). We further examined the effects on free radical scavenging activities in the dried seed extracts. Although scavenging activity correlated well with total phenolic content of samples, no correlation was observed with adiponectin production. These results point to the potential of dried seed extracts as a means to modify the activity of tumor necrosis factor-alpha for the adiponectin production.


Adipocytes Adiponectin DPPH radical scavenging Dried plant seeds Phenolic compounds TNF α 



Adipocyte differentiation medium


Adipocyte maintenance medium


Dulbecco’s modified Eagle’s medium




Preadipocyte medium


Dried plant seed ethanol extracts


Tumor necrosis factor-alpha



This study was supported in part by Grants-in-Aid for Scientific Research from the President of the School of Nursing & Health, Aichi Prefectural University (to Y. Okada). We thank Ms. Wanda Miyata for help with critical review.


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Laboratory on Ageing & Health Management, School of Nursing & HealthAichi Prefectural UniversityNagoyaJapan
  2. 2.Nutrition Section, Ageing and Nutrition ResearchYms LaboratoryGifuJapan
  3. 3.Nagoya University HospitalNagoyaJapan

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