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Sugars Profiles of Different Chestnut (Castanea sativa Mill.) and Almond (Prunus dulcis) Cultivars by HPLC-RI

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Sugar profiles of different almond and chestnut cultivars were obtained by high-performance liquid chromatography (HPLC), by means of a refractive index (RI) detector. A solid-liquid extraction procedure was used in defatted and dried samples. The chromatographic separation was achieved using a Eurospher 100-5 NH2 column using an isocratic elution with acetonitrile/water (70:30, v/v) at a flow rate of 1.0 ml/min. All the compounds were separated in 16 min. The method was optimized and proved to be reproducible and accurate. Generally, more than 95% of sugars were identified for both matrixes. Sugars profiles were quite homogeneous for almond cultivars; sucrose was the main sugar (11.46 ± 0.14 in Marcona to 22.23 ± 0.59 in Ferragnes g/100 g of dried weight), followed by raffinose (0.71 ± 0.05 in Ferraduel to 2.11 ± 0.29 in Duro Italiano), glucose (0.42 ± 0.12 in Pegarinhos two seeded to 1.47 ± 0.19 in Ferragnes) and fructose (0.11 ± 0.02 in Pegarinhos two seeded to 0.59 ± 0.05 in Gloriette). Commercial cultivars proved to have higher sucrose contents, except in the case of Marcona. Nevertheless, chestnut cultivars revealed a high heterogeneity. Sucrose was the main sugar in Aveleira (22.05 ± 1.48), Judia (23.30 ± 0.83) and Longal (9.56 ± 0.91), while glucose was slightly prevalent in Boa Ventura (6.63 ± 0.49). The observed variance could serve for inter-cultivar discrimination.

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  1. Barreira JCM, Ferreira ICFR, Oliveira MBPP, Pereira JA (2008) Antioxidant activity and bioactive compounds of ten Portuguese regional and commercial almond cultivars. Food Chem Toxicol 46:2230–2235

    Article  CAS  Google Scholar 

  2. Balta F, Battal P, Balta MF, Yoruk HI (2009) Free sugar compositions based on kernel taste in almond genotypes Prunus dulcis from Eastern Turkey. Chem Nat Compd 45:221–224

    Article  CAS  Google Scholar 

  3. Cordeiro V, Monteiro A (2001) Almond growing in Trás-os-Montes region (Portugal). Acta Hort 591:161–165

    Google Scholar 

  4. Ahrens S, Venkatachalam M, Mistry AM, Lapsley K, Sathe SK (2005) Almond (Prunus dulcis L.) protein quality. Plant Food Hum Nutr 60:123–128

    Article  CAS  Google Scholar 

  5. Barreira JCM, Casal S, Ferreira ICFR, Oliveira MBPP, Pereira JA (2009) Nutritional, fatty acid and triacylglycerol profiles of Castanea sativa Mill. cultivars: a compositional and chemometric approach. J Agric Food Chem 57:2836–2842

    Article  CAS  Google Scholar 

  6. Borges O, Gonçalves B, Carvalho JLS, Correia P, Silva AP (2008) Nutritional quality of chestnut (Castanea sativa Mill.) cultivars from Portugal. Food Chem 106:976–984

    Article  CAS  Google Scholar 

  7. Kazantzis I, Nanos GD, Stavroulakis GG (2003) Effect of harvest time and storage conditions on almond kernel oil and sugar composition. J Sci Food Agric 83:354–359

    Article  CAS  Google Scholar 

  8. Barreira JCM, Alves RC, Casal S, Ferreira ICFR, Oliveira MBPP, Pereira JA (2009) Vitamin E profile as a reliable authenticity discrimination factor between Chestnut (Castanea sativa Mill.) cultivars. J Agric Food Chem 57:5524–5528

    Article  CAS  Google Scholar 

  9. Barreira JCM, Ferreira ICFR, Oliveira MBPP, Pereira JA (2008) Antioxidant activities of the extracts from chestnut flower, leaf, skins and fruit. Food Chem 107:1106–1113

    Article  CAS  Google Scholar 

  10. Künsch U, Schärer H, Patrian B, Höhn E, Conedera M, Sassella A, Jermini M, Jelmini G (2001) Effects of roasting on chemical composition and quality of different chestnut (Castanea sativa Mill.) varieties. J Sci Food Agric 81:1106–1112

    Article  Google Scholar 

  11. Bernárdez MM, Miguélez JM, Queijeiro JG (2004) HPLC determination of sugars in varieties of chestnut fruits from Galicia (Spain). J Food Comp Anal 17:63–67

    Article  Google Scholar 

  12. Nanos GD, Kazantzis I, Kefalas P, Petrakis C, Stavroulakis GG (2002) Irrigation and harvest time affect almond kernel quality and composition. Sci Hortic 96:249–256

    Article  CAS  Google Scholar 

  13. Saura-Calixto F, Canellas J, Raso-Garcia A (1984) Gas chromatographic analysis of sugars and sugar-alcohols in the mesocarp, endocarp and kernel of almond fruit. J Agric Food Chem 32:1018–1020

    Article  CAS  Google Scholar 

  14. Soler L, Canellas J, Saura-Calixto F (1989) Changes in carbohydrate and protein content and composition of developing almond seeds. J Agric Food Chem 37:1400–1404

    Article  CAS  Google Scholar 

  15. Bliss FA (1999) Nutritional improvement of horticultural crops through plant breeding. Hortscience 34:1163–1167

    Google Scholar 

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Foundation for Science and Technology (Portugal) for financial support to J.C.M. Barreira (SFRH/BD/29060/2006) and INTERREG IIIA project PIREFI.

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Correspondence to Isabel C. F. R. Ferreira.

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Barreira, J.C.M., Pereira, J.A., Oliveira, M.B.P.P. et al. Sugars Profiles of Different Chestnut (Castanea sativa Mill.) and Almond (Prunus dulcis) Cultivars by HPLC-RI. Plant Foods Hum Nutr 65, 38–43 (2010).

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