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Antioxidant and Antiproliferative Activity of Diospyros lotus L. Extract and Isolated Compounds

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Abstract

The object of the study was to determine the chemical composition of Diospyros lotus L. extract and their antioxidant and antiproliferative properties. Eight compounds were isolated from D. lotus and identified as gallic acid, methylgallate, ellagic acid, kaempferol, quercetin, myricetin, myricetin 3-O-β-glucuronide, and myricetin-3-O-α-rhamnoside. D. lotus extract tested in different in vitro systems (DPPH, ABTS, FRAP, and Fe2+ chelating activity assay) showed significant antioxidant activity. The potential antiproliferative properties of D. lotus extract and isolated compounds against nine human cancer cell lines such as COR-L23, CaCo-2, C32, ACHN, A375, A549, Huh-7D12, MCF-7, and LNCaP were investigated in vitro by SRB assay. D. lotus extract demonstrated the highest inhibitory activity against COR-L23 with an IC50 value of 12.2 μg/ml. Among identified hydrolysable tannins, ellagic acid evidenced strong antiproliferative activity against both C32 and A375 cells with IC50 values of 0.8 and 4.1 μg/ml, respectively. Interesting results were observed, also, with gallic acid that showed the highest cytotoxic activity against CaCo-2 (IC50 2.6 μg/ml). Overall, the results of this study suggest that D. lotus displays a good antioxidant activity and has antiproliferative effects. Both activities are related to identified phenolic compounds.

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Abbreviations

ABTS:

2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

DPPH:

2,2-Diphenyl-1-picrylhydrazyl

FRAP:

Ferric Reducing Ability Power

ROS:

Reactive oxygen species

SRB:

Sulforhodamine B

TEAC:

Trolox Equivalent Antioxidant Capacity

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Acknowledgment

The authors are grateful to Dr. V. Filippelli, Faculty of Pharmacy, Nutrition and Health Science, University of Calabria, for English revision of the manuscript.

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, Faculty of Pharmacy, Nutrition and Health Sciences, University of Calabria, 87036, Rende, CS, Italy

    Monica Rosa Loizzo, Rosa Tundis, Federica Menichini & Francesco Menichini

  2. Pharmacognosy Department, National Research Centre, Dokki, Giza, Egypt

    Ataa Said & Khaled Rashed

  3. Phytochemistry and Plant Systematic Department, National Research Centre, Dokki, Giza, Egypt

    Usama W. Hawas

  4. Department of Food Science, Politecnica delle Marche University, via Brecce Bianche, 60131, Ancona, Italy

    Natale Giuseppe Frega

Authors
  1. Monica Rosa Loizzo
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  2. Ataa Said
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  3. Rosa Tundis
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  6. Federica Menichini
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  8. Francesco Menichini
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Correspondence to Monica Rosa Loizzo.

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Loizzo, M.R., Said, A., Tundis, R. et al. Antioxidant and Antiproliferative Activity of Diospyros lotus L. Extract and Isolated Compounds. Plant Foods Hum Nutr 64, 264–270 (2009). https://doi.org/10.1007/s11130-009-0133-0

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  • DOI: https://doi.org/10.1007/s11130-009-0133-0

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