Antioxidant and Immunity Activity of Water Extract and Crude Polysaccharide from Ficus carica L. Fruit

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The antioxidative activities of water extract (WE) and crude hot-water soluble polysaccharide (PS) from Ficus carica L. fruit were investigated using various assays in vitro, including scavenging abilities on DPPH, superoxide and hydroxyl radicals and reducing power. The immunity activities of PS were evaluated using the carbon clearance test and serum hemolysin analysis in mice. In addition, total phenolics and flavonoids contents were also determined. Both WE and PS have notable scavenging activities on DPPH with the EC50 values of 0.72 and 0.61 mg/ml, respectively. The PS showed higher scavenging activity than WE on superoxide radical (EC50, 0.95 mg/ml) and hydroxyl anion radical (scavenging rate 43.4% at concentration of 4 mg/ml). The PS (500 mg/kg) also has a significant increase in the clearance rate of carbon particles and serum hemolysin level of normal mice. The results indicate that both WE and PS might be applicable in healthy medicine and food industry.

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Fig. 1
Fig. 2
Fig. 3



water extract of Ficus carica fruit


crude hot water-soluble polysaccharide of Ficus carica fruit


trolox equivalent antioxidant capacity


phosphorous buffered saline


2, 2-diphenyl-1-picryl-hydrazyl


nitro blue tetrazolium


nicotinamide adenine dinucleotide-reduced


ascorbic acid


phenazin methosulphate


butylated hydroxytoluene


chook red blood cells


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Correspondence to Xiao-ming Yang.

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Yang, X., Yu, W., Ou, Z. et al. Antioxidant and Immunity Activity of Water Extract and Crude Polysaccharide from Ficus carica L. Fruit. Plant Foods Hum Nutr 64, 167–173 (2009) doi:10.1007/s11130-009-0120-5

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  • Ficus carica L
  • Extract
  • Polysaccharide
  • Antioxidant activity
  • Immunity activity