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Antihyperglycemic, Antioxidant and Antiglycation Activities of Mulberry Leaf Extract in Streptozotocin-Induced Chronic Diabetic Rats

Plant Foods for Human Nutrition volume 64, pages 116–121 (2009)Cite this article

Abstract

In Thailand, beverages containing mulberry leaf (Morus alba L.) are believed to promote good health, especially in people with diabetes. We examined the effects of long-term administration of an ethanolic extract of mulberry leaf (MA) on blood glucose, oxidative damage, and glycation in streptozotocin-induced diabetic rats. Daily administration of 1 g/kg MA for six weeks decreased blood glucose by 22%, which was comparable to the effect of 4 U/kg insulin. Lipid peroxidation, measured as malondialdehyde and lipid hydroperoxide concentrations (3.50 ± 0.33 and 3.76 ± 0.18 μM, respectively) decreased significantly (P < 0.05) compared to nontreated control diabetic rats (8.19 ± 0.45 and 7.50 ± 0.46 μM, respectively). Hemoglobin A1C, a biomarker for chronic exposure to high concentration of glucose, was also significantly decreased in the MA-treated group (6.78 ± 0.30%) in comparison to untreated group (9.02 ± 0.30%). The IC50 of in vitro antiglycation and free radical scavenging activities of MA were 16.4 ± 5.6 μg/ml and 61.7 ± 2.1 μg/ml, respectively. These findings support that long-term administration of MA has antihyperglycemic, antioxidant and antiglycation effects in chronic diabetic rats, which may be beneficial as food supplement for diabetics.

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Abbreviations

AGEs:

Advanced glycation endproducts

MA:

Morus alba-ethanolic extract

MDA:

Malondialdehyde

STZ:

Streptozotocin

References

  1. Nathan DM, Cleary PA, Backlund JY, Genuth SM, Lachin JM, Orchard TJ, Raskin P, Zinman B (2005) Intensive diabetes treatment and cardiovascular disease in patients with type 1 diabetes. N Engl J Med 353:2643–2653. doi:10.1056/NEJMoa052187

    Article  Google Scholar 

  2. Brownlee M (2001) Biochemistry and molecular cell biology of diabetic complications. Nature 414:813–820. doi:10.1038/414813a

    Article  CAS  Google Scholar 

  3. Jakus V, Rietbrock N (2004) Advanced glycation end-products and the progress of diabetic vascular complications. Physiol Res 53:131–142

    CAS  Google Scholar 

  4. Ahmed N (2005) Advanced glycation endproducts—role in pathology of diabetic complications. Diabetes Res Clin Pract 67:3–21. doi:10.1016/j.diabres.2004.09.004

    Article  CAS  Google Scholar 

  5. Baynes JW, Thorpe SR (2000) Glycoxidation and lipoxidation in atherogenesis. Free Radic Biol Med 28:1708–1716. doi:10.1016/S0891-5849(00) 00228-8

    Article  CAS  Google Scholar 

  6. Idris I, Gray S, Donnelly R (2001) Protein kinase C activation: isozyme-specific effects on metabolism and cardiovascular complications in diabetes. Diabetologia 44:659–673. doi:10.1007/s001250051675

    Article  CAS  Google Scholar 

  7. Singab AN, El-Beshbishy HA, Yonekawa M, Nomura T, Fukai T (2005) Hypoglycemic effect of Egyptian Morus alba root bark extract: effect on diabetes and lipid peroxidation of streptozotocin-induced diabetic rats. J Ethnopharmacol 100:333–338. doi:10.1016/j.jep. 2005.03.013

    Article  CAS  Google Scholar 

  8. Draper HH, Squires EJ, Mahmoodi H, Wu J, Agarwal S, Hadley M (1993) A comparative evaluation of thiobarbituric acid methods for the determination of malondialdehyde in biological materials. Free Radic Biol Med 15:353–363. doi:10.1016/0891-5849(93)90035-S

    Article  CAS  Google Scholar 

  9. Nourooz-Zadeh J, Tajaddini-Sarmadi J, Wolff SP (1994) Measurement of plasma hydroperoxide concentrations by the ferrous oxidation-xylenol orange assay in conjunction with triphenylphosphine. Anal Biochem 220:403–409. doi:10.1006/abio.1994.1357

    Article  CAS  Google Scholar 

  10. Little RR, Wiedmeyer HM, England JD, Wilke AL, Rohlfing CL, Wians FH Jr, Jacobson JM, Zellmer V, Goldstein DE (1992) Interlaboratory standardization of measurements of glycohemoglobins. Clin Chem 38:2472–2478

    CAS  Google Scholar 

  11. Yokozawa T, Nakagawa T (2004) Inhibitory effects of Luobuma tea and its components against glucose-mediated protein damage. Food Chem Toxicol 42:975–981. doi:10.1016/j.fct.2004.02.010

    Article  CAS  Google Scholar 

  12. Awika JM, Rooney LW, Wu X, Prior RL, Cisneros-Zevallos L (2003) Screening methods to measure antioxidant activity of sorghum (sorghum bicolor) and sorghum products. J Agric Food Chem 51:6657–6662. doi:10.1021/jf034790i

    Article  CAS  Google Scholar 

  13. Ozturk Y, Altan VM, Yildizoglu-Ari N (1996) Effects of experimental diabetes and insulin on smooth muscle functions. Pharmacol Rev 48:69–112

    CAS  Google Scholar 

  14. Hansawasdi C, Kawabata J (2006) Alpha-glucosidase inhibitory effect of mulberry (Morus alba) leaves on Caco-2. Fitoterapia 77:568–573. doi:10.1016/j.fitote.2006.09.003

    Article  Google Scholar 

  15. Hussain Z, Waheed A, Qureshi RA, Burdi DK, Verspohl EJ, Khan N, Hasan M (2004) The effect of medicinal plants of Islamabad and Murree region of Pakistan on insulin secretion from INS-1 cells. Phytother Res 18:73–77. doi:10.1002/ptr.1372

    Article  Google Scholar 

  16. Stratton IM, Adler AI, Neil HA, Matthews DR, Manley SE, Cull CA, Hadden D, Turner RC, Holman RR (2000) Association of glycaemia with macrovascular and microvascular complications of type 2 diabetes (UKPDS 35): prospective observational study. BMJ 321:405–412. doi:10.1136/bmj.321.7258.405

    Article  CAS  Google Scholar 

  17. Oh H, Ko EK, Jun JY, Oh MH, Park SU, Kang KH, Lee HS, Kim YC (2002) Hepatoprotective and free radical scavenging activities of prenylflavonoids, coumarin, and stilbene from Morus alba. Planta Med 68:932–934. doi:10.1055/s-2002-34930

    Article  CAS  Google Scholar 

  18. Kaviarasan S, Vijayalakshmi K, Anuradha CV (2004) Polyphenol-rich extract of fenugreek seeds protect erythrocytes from oxidative damage. Plant Foods Hum Nutr 59:143–147. doi:10.1007/s11130-004-0025-2

    Article  CAS  Google Scholar 

  19. Vecera R, Orolin J, Skottova N, Kazdova L, Oliyarnik O, Ulrichova J, Simanek V (2007) The influence of maca (Lepidium meyenii) on antioxidant status, lipid and glucose metabolism in rat. Plant Foods Hum Nutr 62:59–63. doi:10.1007/s11130-007-0042-z

    Article  Google Scholar 

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Acknowledgements

This work was supported by the Faculty of Medicine and Graduate School, Khon Kaen University (Grant number i50111). Miss Jarinyaporn Naowaboot is a graduate student supported by the Royal Golden Jubilee Ph.D. Program Grant, Thailand Research Fund.

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Authors and Affiliations

  1. Department of Pharmacology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand

    Jarinyaporn Naowaboot, Patchareewan Pannangpetch, Veerapol Kukongviriyapan & Bunkerd Kongyingyoes

  2. Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand

    Upa kukongviriyapan

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  1. Jarinyaporn Naowaboot
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  2. Patchareewan Pannangpetch
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  3. Veerapol Kukongviriyapan
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Correspondence to Patchareewan Pannangpetch.

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Naowaboot, J., Pannangpetch, P., Kukongviriyapan, V. et al. Antihyperglycemic, Antioxidant and Antiglycation Activities of Mulberry Leaf Extract in Streptozotocin-Induced Chronic Diabetic Rats. Plant Foods Hum Nutr 64, 116–121 (2009). https://doi.org/10.1007/s11130-009-0112-5

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Keywords

  • Antiglycation
  • Antioxidant
  • Diabetes mellitus
  • Morus alba
  • Mulberry leaf