Extensive Screening for Plant Foodstuffs in Okinawa, Japan with Anti-Obese Activity on Adipocytes In Vitro
- 415 Downloads
To search for plant foodstuffs with potent anti-obese activity, we conducted a large scale screening based on the inhibitory activity on adipogenesis and the facilitating activity on adipolysis in vitro. That is, inhibition of intracellular lipid accumulation and facilitation of lipid degradation in 3T3-L1 adipocytes were extensively screened from ethanol and hexane extracts of approximately 100 kinds of plant foodstuffs marketed in Okinawa prefecture, which has been famous for the highest prevalence of exceptionally long-lived individuals in the world. Among them thirty one foodstuffs showed potent inhibitory activity on intracellular lipid accumulation in 3T3-L1 adipocytes, whereas only four foodstuffs showed clear facilitating effect on lipid degradation in 3T3-L1 adipocytes. Although further study to examine the in vivo effects on adipogenesis and adipolysis is required, this is the first study to investigate anti-obese characteristics of wide range of traditional Okinawa foodstuffs so that the results give useful information to take another look at Okinawa food culture.
KeywordsOkinawa foodstuffs Anti-obese activity 3T3-L1 adipocyte
Dulbecco’s modified Eagle’s medium
fetal calf serum
This work was supported by a grant of the Industry-Academia-Government Collaboration, Okinawa 2006.
- 3.Willcox BJ, Willcox DG, Todoriki H, Fujiyoshi A, Yano K, He Q, Curb JD, Suzuki M (2007) Caloric restriction, the traditional Okinawa diet, and healthy aging. The diet of the world’s longest-lived people and its potential impact on morbidity and life span. N Y Acad Sci 1114:434–455. doi: 10.1196/annals.1396.037 CrossRefGoogle Scholar
- 4.Tanaka H, Shimabukuro T, Shimabukuro M (2005) High prevalence of metabolic syndorome among men in Okinawa. J Atheroscler Thromb 12:284–288Google Scholar
- 5.Gregoire FM, Smas CM, Sul HS (1998) Understanding adipocyte differentiation. Physiol Rev 78:783–809Google Scholar
- 6.Rosen ED, Walkey CJ, Puigserver P, Spiegelman BM (2000) Transcriptional regulation of adipogenesis. Genes Dev 14:1293–1307Google Scholar
- 15.Avula B, Upparapalli SK, Khan IA (2007) Simultaneous analysis of adrenergic amines and flavonoids in citrus peel jams abd fruit juices by liquid chromatography: part 2. J AOAC Int 90:633–640Google Scholar
- 16.Jordan R, Midgley JM, Thonoor CM, Williams CM (1987) Beta-adrenergic activities of octopamine and synephrine stereoisomaers on guinea-pig atria and trachea. J Pharm Pharmacol 39:752–754Google Scholar
- 19.Sundbom M, Kaiser C, Björkstrand E, Castro VM, Larsson C, Selén G, Nyhem CS, James SR (2008) Inhibition of 11betaHSD1 with the S-phenylethylaminothiazolone BVT116429 increases adiponectin concentrations and improves glucose homeostasis in diabetic KKAy mice. BMC Pharmacol 8:3. doi: 10.1186/1471-2210-8-3 CrossRefGoogle Scholar
- 21.Masuzaki H, Ogawa Y, Aizawa-Abe M, Hosoda K, Suga J, Ebihara K, Satoh N, Iwai H, Inoue G, Nishimura H, Yoshimasa Y, Nakao K (1999) Glucose metabolism and insulin sensitivity in transgenic mice overexpressing leptin with lethal yellow agouti mutation: usefulness of leptin for the treatment of obesity-associated diabetes. Diabetes 48:1615–1622. doi: 10.2337/diabetes.48.8.1615 CrossRefGoogle Scholar