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Ingestion of Black Chokeberry Fruit Extract Leads to Intestinal and Systemic Changes in a Rat Model of Prediabetes and Hyperlipidemia

Plant Foods for Human Nutrition volume 63, pages 176–182 (2008)Cite this article

Abstract

This report presents a complex analysis of changes proceeding in the gut, blood and internal organs of rats with induced oxidative stress, glucose intolerance and hyperlipidemia after dietary supplementation with an extract from black chokeberry (Aronia melanocarpa) fruit, that is a condensed source of polyphenols (714 mg/g), especially anthocyanin glycosides (56.6%). The disturbances mimicking those observed in metabolic syndrome were induced by a high-fructose diet and simultaneous single injection of streptozotocin (20 mg/kg). Dietary supplementation with the chokeberry fruit extract (0.2%) decreased activity of maltase and sucrase as well as increased activity of lactase in the mucosa of the small intestine. Its ingestion led also to the improvement of antioxidant status, especially, the concentration of a lipid peroxidation indicator (TBARS) in organ tissues (liver, kidney and lung) was normalized; some cholesterol-lowering and distinct hypoglycemic actions were also observed. The mechanism of glucose reduction is likely to be multifactorial, and we suggest the factors related with the decreased activity of mucosal disaccharidases important for further investigation. In conclusion, chokeberry fruit derivatives may act as a promising supplementary therapeutic option in the prevention and treatment of disorders occurring in metabolic syndrome, as well as their complications.

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Abbreviations

ACL:

antioxidant capacity of lipophilic substances

ACH:

antioxidant capacity of hydrophilic substances

STZ:

streptozotocin

GPx:

glutathione peroxidase

HDL:

high-density lipoprotein

SCFA:

short-chain fatty acids

SOD:

superoxide dismutase

TBARS:

thiobarbituric acid-reactive substances

References

  1. Persson Hovmalm HA, Jeppsson N, Bartish IV, Nybom H (2004) RAPD analysis of diploid and tetraploid populations of Aronia points to different reproductive strategies within the genus. Hereditas 141:301–312

    Article  Google Scholar 

  2. Sueiro L, Yousef GG, Seigler D, de Mejia EG, Grace MH, Lila MA (2006) Chemopreventive potential of flavonoid extracts from plantation-bred and wild Aronia melanocarpa (Black chokeberry) fruits. J Food Sci 71:480–488

    Article  Google Scholar 

  3. Valcheva-Kuzmanova S, Borisova P, Galunska B, Krasnaliev I, Belcheva A (2004) Hepatoprotective effect of the natural fruit juice from Aronia melanocarpa on carbon tetrachloride-induced acute liver damage in rats. Exp Toxicol Pathol 56:195–201

    Article  CAS  Google Scholar 

  4. Valcheva-Kuzmanova S, Kuzmanov K, Mihova V, Krasnaliev I, Borisova P, Belcheva A (2007a) Antihyperlipidemic effect of Aronia melanocarpa fruit juice in rats fed a high cholesterol diet. Plant Food Hum Nutr 62:19–24

    Article  CAS  Google Scholar 

  5. Valcheva-Kuzmanova S, Kuzmanov K, Tancheva S, Belcheva A (2007b) Hypoglycemic and hypolipidemic effects of Aronia melanocarpa fruit juice in streptozotocin-induced diabetic rats. Method Find Exp Clin Pharmacol 29:1–5

    Google Scholar 

  6. Ryszawa N, Kawczyńska-Dróżdż A, Pryjma J, Czesnikiewicz-guzik M, Adamek-guzik T, Naruszewicz M, Korbut R, Guzik TJ (2006) Effects of novel plant antioxidants on platelet superoxide production and aggregation in atherosclerosis. J Physiol Pharmacol 57:611–626

    CAS  Google Scholar 

  7. Naruszewicz M, Łaniewska I, Millo B, Dłużniewski M (2007) Combination therapy of statin with flavonoids rich extract from chokeberry fruits enhanced reduction in cardiovascular risk markers in patients after myocardial infraction (MI). Atherosclerosis 194:79–184

    Article  Google Scholar 

  8. Ohgami K, Ilieva I, Shiratori K, Koyama Y, Jin X-H, Yoshida K, Kase S, Kitaichi N, Suzuki Y, Tanaka T, Ohno S (2005) Effects of novel plant antioxidants on platelet superoxide production and aggregation in atherosclerosis. Invest Ophth Vis Sci 46:275–281

    Article  Google Scholar 

  9. Bell DR, Gochenaur K (2006) Direct vasoactive and vasoprotective properties of anthocyanin-rich extracts. J Appl Physiol 100:1164–1170

    Article  CAS  Google Scholar 

  10. Després J-P, Lemieux I (2006) Abdominal obesity and metabolic syndrome. Nature 444:881–887

    Article  Google Scholar 

  11. Busserolles J, Gueux E, Rock E, Demigne C, Mazur A, Rayssiguier Y (2003) Oligofructose protects against the hypertriglyceridemic and pro-oxidative effects of a high fructose diet in rats. J Nutr 133:1903–1908

    CAS  Google Scholar 

  12. Garg MC, Ojha S, Bansal DD (1996) Antioxidant status of streptozotocin diabetic rats. Indian J Exp Biol 34:264–266

    CAS  Google Scholar 

  13. Oszmianski J, Wojdylo A (2005) Aronia melanocarpa phenolics and their antioxidant activity. Eur Food Res Technol 221:809–813

    Article  CAS  Google Scholar 

  14. Frejnagel S (2007) Comparison of polyphenolic composition of extracts from honeysuckle, chokeberries and green tea—a short report. Pol J Food Nutr Sci 57:83–86

    CAS  Google Scholar 

  15. Dahlqvist A (1964) Method for assay of intestinal disaccharidases. Anal Biochem 7:18–25

    Article  CAS  Google Scholar 

  16. Hofirek B, Haas D (2001) Comparative studies of ruminal fluid collected by oral tube or by puncture of the caudorental ruminal sac. Acta Vet Brno 70:27–33

    Google Scholar 

  17. Juśkiewicz J, Zduńczyk Z (2004) Effects of cellulose, carboxymethylcellulose and inulin fed to rats as single supplements or in combinations on their caecal parameters. Comp Biochem Phys A 139:513–519

    Article  Google Scholar 

  18. Uchiyama M, Mihara M (1978) Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem 86:271–278

    Article  CAS  Google Scholar 

  19. Girard A, Madani S, El Boustani ES, Belleville J, Prost J (2005) Changes in lipid metabolism and antioxidant defense status in spontaneously hypertensive rats and Wistar rats fed a diet enriched with fructose and saturated fatty acids. Nutrition 21:240–248

    Article  CAS  Google Scholar 

  20. Chavez M, Seeley RJ, Havel PJ, Friedman MI, Matson CA, Woods SC, Schwartz MW (1998) Effect of a high-fat diet on food intake and hypothalamic neuropeptide gene expression in streptozotocin diabetes. J Clin Invest 102:340–346

    Article  CAS  Google Scholar 

  21. Okada M, Shibuya M, Yamamoto E, Murakami Y (1999) Effect of diabetes on vitamin B6 requirement in experimental animals. Diabetes Obes Metab 1:221–225

    Article  CAS  Google Scholar 

  22. Benvenuti S, Pellati F, Melegari M, Bertelli D (2004) Polyphenols, anthocyanins, ascorbic acid, and radical scavenging activity of rubus, ribes, and aronia. J Food Sci 69:164–169

    Google Scholar 

  23. Adachi T, Mori C, Sakurai K, Shihara N, Tsuda K, Yasuda K (2003) Morphological changes and increased sucrase and isomaltase activity in small intestines of insulin deficient and type 2 diabetic rats. Endocr J 50:271–279

    Article  CAS  Google Scholar 

  24. Day AJ, Gee JM, DuPont MS, Johnson IT, Williamson G (2003) Absorption of quercetin-3-glucoside and quercetin-4′-glucoside in the rat small intestine: the role of lactase phlorizin hydrolase and the sodium-dependent glucose transporter. Biochem Pharmacol 65:1199–1206

    Article  CAS  Google Scholar 

  25. Murakami I, Ikeda T (1998) Effects of diabetes and hyperglycemia on disaccharidase activities in the rat. Scand J Gastroentero 33:1069–1073

    Article  CAS  Google Scholar 

  26. Skoog SM, Bharucha AE (2004) Dietary fructose and gastrointestinal symptoms: a review. Am J Gastroenterol 99:2046–2050

    Article  CAS  Google Scholar 

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Affiliations

  1. Department of Biological Analysis of Food, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-747, Olsztyn, Poland

    Adam Jurgoński, Jerzy Juśkiewicz & Zenon Zduńczyk

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  1. Adam Jurgoński
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  2. Jerzy Juśkiewicz
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  3. Zenon Zduńczyk
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Correspondence to Adam Jurgoński.

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Jurgoński, A., Juśkiewicz, J. & Zduńczyk, Z. Ingestion of Black Chokeberry Fruit Extract Leads to Intestinal and Systemic Changes in a Rat Model of Prediabetes and Hyperlipidemia. Plant Foods Hum Nutr 63, 176–182 (2008). https://doi.org/10.1007/s11130-008-0087-7

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  • DOI: https://doi.org/10.1007/s11130-008-0087-7

Keywords

  • Anthocyanins
  • Metabolic syndrome
  • Disaccharidase
  • Polyphenols
  • Rat model