Abstract
The antioxidative effect of the carotenoids phytoene and phytofluene in biological systems has not yet been studied. We therefore sought to investigate the effect of these carotenoids, isolated from the alga Dunaliella bardawil, in a biological system and used the in vitro low density lipoprotein (LDL) oxidation method to assay their antioxidative effects. We found that similar to β-carotene and α-tocopherol, a carotenoid algal preparation containing phytoene and phytofluene inhibited LDL oxidation. These findings and the presence of phytoene and phytofluene in human tissues suggest that they can be part of the defense system against oxidative stress.
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References
Gaziano JM (1999) Antioxidant vitamins and cardiovascular disease. Proc Assoc Am Physicians 111:2–9
Blumberg J, Block G (1994) The alpha-tocopherol, beta-carotene cancer prevention study in Finland. Nutr Rev 52:242–245
Hennekens CH et al (1996) Lack of effect of long-term supplementation with beta carotene on the incidence of malignant neoplasms and cardiovascular disease. N Engl J Med 334:1145–1149
Cook NR et al (2007) A randomized factorial trial of vitamins C and E and beta carotene in the secondary prevention of cardiovascular events in women: results from the Women’s Antioxidant Cardiovascular Study. Arch Intern Med 167:1610–1618
Omenn GS et al (1996) Risk factors for lung cancer and for intervention effects in CARET, the Beta-Carotene and Retinol Efficacy Trial. J Natl Cancer Inst 88:1550–1559
Albanes D et al (1996) Alpha-tocopherol and beta-carotene supplements and lung cancer incidence in the alpha-tocopherol, beta-carotene cancer prevention study: effects of base-line characteristics and study compliance. J Natl Cancer Inst 88:1560–1570
Nara E et al (2001) Acyclic carotenoids and their oxidation mixtures inhibit the growth of HL-60 human promyelocytic leukemia cells. Nutr Cancer 39:273–283
Fuller B et al (2006) Anti-inflammatory effects of CoQ10 and colorless carotenoids. J Cosmet Dermatol 5:30–38
Ben-Dor A et al (2005) Carotenoids activate the antioxidant response element transcription system. Mol Cancer Ther 4:177–186
Hirsch K et al (2007) Lycopene and other carotenoids inhibit estrogenic activity of 17beta-estradiol and genistein in cancer cells. Breast Cancer Res Treat 104:221–230
Esterbauer H et al (1989) The role of vitamin E and carotenoids in preventing oxidation of low density lipoproteins. Ann N Y Acad Sci 570:254–267
Aust O et al (2005) Supplementation with tomato-based products increases lycopene, phytofluene, and phytoene levels in human serum and protects against UV-light-induced erythema. Int J Vitam Nutr Res 75:54–60
Khachik F et al (2002) Chemistry, distribution, and metabolism of tomato carotenoids and their impact on human health. Exp Biol Med (Maywood) 227:845–851
Werman MJ et al (2002) Bioavailability of the isomer mixture of phytoene and phytofluene-rich alga Dunaliella bardawil in rat plasma and tissues. J Nutr Biochem 13:585–591
Markwell MA et al (1978) A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem 87:206–210
Shaish A et al (1995) Beta-carotene inhibits atherosclerosis in hypercholesterolemic rabbits. J Clin Invest 96:2075–2082
Shaish A et al (1999) Dietary beta-carotene and alpha-tocopherol combination does not inhibit atherogenesis in an ApoE-deficient mouse model. Arterioscler Thromb Vasc Biol 19:1470–1475
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Shaish, A., Harari, A., Kamari, Y. et al. A Carotenoid Algal Preparation Containing Phytoene and Phytofluene Inhibited LDL Oxidation In Vitro . Plant Foods Hum Nutr 63, 83–86 (2008). https://doi.org/10.1007/s11130-008-0075-y
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DOI: https://doi.org/10.1007/s11130-008-0075-y