Antioxidative and Anticancer Activity of Extracts of Cherry (Prunus serrulata var. spontanea) Blossoms

Abstract

Organic solvent (methanol, ethanol, and acetone) extracts and water extracts of cherry (Prunus serrulata var. spontanea) blossoms were prepared, and antioxidant activities of the extracts were evaluated. Methanolic CBE (100 μg/ml) showed the highest total phenol content (104.30 μM), radical scavenging activity (34.2%), and reducing power (0.391). The effect of CBE on DNA damage induced by H2O2 in human leukocytes was evaluated by Comet assay. All CBE was a potent dose dependent inhibitor of DNA damage induced by 200 μM of H2O2, methanolic CBE showed the most strong inhibition activity. The methanolic CBE of 500 μg/ml showed 38.8% inhibition against growth of human colon cancer cell line HT-29. These results indicated that cherry blossoms could provide valuable bioactive materials.

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Abbreviations

CB:

cherry blossoms

CBE:

cherry blossom extract

DMSO:

dimethyl sulfoxide

DPPH:

1,1-diphenyl-2-picrylhydrazyl

LMA:

low melting agarose

MTT:

3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide

PBS:

phosphorous buffered saline

ROS:

reactive oxygen species

RP:

reducing power

RSA:

radical scavenging activity

TPC:

total phenolic content

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Acknowledgements

This study was supported by Kyungnam University Research Fund, 2007.

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Correspondence to Seung-Cheol Lee.

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Lee, B., Cha, M., Kim, S. et al. Antioxidative and Anticancer Activity of Extracts of Cherry (Prunus serrulata var. spontanea) Blossoms. Plant Foods Hum Nutr 62, 79 (2007). https://doi.org/10.1007/s11130-007-0045-9

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Keywords

  • Prunus serrulata var. spontanea
  • Extract
  • Antioxidant
  • Anticancer