Organic solvent (methanol, ethanol, and acetone) extracts and water extracts of cherry (Prunus serrulata var. spontanea) blossoms were prepared, and antioxidant activities of the extracts were evaluated. Methanolic CBE (100 μg/ml) showed the highest total phenol content (104.30 μM), radical scavenging activity (34.2%), and reducing power (0.391). The effect of CBE on DNA damage induced by H2O2 in human leukocytes was evaluated by Comet assay. All CBE was a potent dose dependent inhibitor of DNA damage induced by 200 μM of H2O2, methanolic CBE showed the most strong inhibition activity. The methanolic CBE of 500 μg/ml showed 38.8% inhibition against growth of human colon cancer cell line HT-29. These results indicated that cherry blossoms could provide valuable bioactive materials.
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cherry blossom extract
low melting agarose
phosphorous buffered saline
reactive oxygen species
radical scavenging activity
total phenolic content
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This study was supported by Kyungnam University Research Fund, 2007.
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Lee, B., Cha, M., Kim, S. et al. Antioxidative and Anticancer Activity of Extracts of Cherry (Prunus serrulata var. spontanea) Blossoms. Plant Foods Hum Nutr 62, 79 (2007). https://doi.org/10.1007/s11130-007-0045-9
- Prunus serrulata var. spontanea