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Commercial Soy Protein Ingredients as Isoflavone Sources for Functional Foods

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Abstract.

The knowledge of the contents and profile of isoflavones present in soy protein ingredients, as well as the effect of industrial processing, is important for the development of functional foods rich in these compounds. The results obtained here showed that the total isoflavone content varied significantly among products. For defatted and whole soy flours the total isoflavone content ranged from 120 to 340 mg/100 g, for soy protein isolates from 88 to 164 mg/100 g, and for commercial textured soy proteins, from 66 to 183 mg/100 g (wet basis, expressed as aglycones). The highest isoflavone content was found for soy hypocotyl flours, from 542 to 851 mg/100 g. Compared to hypocotyl and whole and defatted flours, soy ingredients presented a decrease of malonylglycosides and deesterified β-glycosides with a significant increase in the percentage of aglycones, mainly for soy fibers (65–76%). While defatting was shown to cause isoflavone concentration without altering conjugation, extrusion process caused destruction of isoflavones and a significant increase in the amount of acetylglycosides, but this effect was less intense for the concentrates. From the results obtained it can be concluded that differences in isoflavone concentration and profile may be related to oscillations in the isoflavone content present in the raw material and to the type of processing.

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References

  1. Adlercreutz H, Mazur W (1997) Phyto-oestrogens and Western diseases. Ann Med 29: 95–120.

    CAS  Google Scholar 

  2. Faraj A, Vasanthan T (2004) Soybean isoflavones: Effects of processing and health benefits. Food Rev Int 20(1): 51–75.

    Article  CAS  Google Scholar 

  3. Naim M, Gestetner B, Bondi A, Birk Y (1976) Antioxidative and antihemolytic activities of soybean isoflavones. J Agric Food Chem 24: 1174–1177.

    Article  CAS  Google Scholar 

  4. Xu X, Wang HJ, Murphy PA, Cook L, Hendrich S (1994) Daidzein is a more bioavailable soymilk isoflavone than is genistein in adult women. J Nutr 124(6): 825–832.

    CAS  Google Scholar 

  5. Setchell KDR, Brown NM, Desai P, Zimmer-Nechemias L, Wolfe BE, Brashear WT, Kirschner AS, Cassidy A, Heubi JE (2001) Bioavailability of pure isoflavones in healthy humans and analysis of commercial soy isoflavone supplements. J Nutr 131: 1362S–1375S.

    CAS  Google Scholar 

  6. Genovese MI, Lajolo FM (2001a) Isoflavonas da soja: fatores que influem nos tipos e teores em alimentos. Food Ingred 11: 62–64.

    Google Scholar 

  7. Genovese MI, Lajolo FM (2001b) Determinação de isoflavonas em derivados de soja. Ciênc Tecnol Alim 21(1): 86–93.

    CAS  Google Scholar 

  8. Genovese MI, Lajolo FM (2002) Isoflavones in soy-based foods consumed in Brazil: levels, distribution, and estimated intake. J Agric Food Chem 50(21): 5987–5993.

    Article  CAS  Google Scholar 

  9. Genovese MI, Hassimotto NMA, Lajolo FM (2005) Isoflavone profile and antioxidant activity of Brazilian soybeans varieties. Food Sci Tech Int 11(3): 205–211.

    Article  CAS  Google Scholar 

  10. Zhang YC, Albrecht D, Bomser J, Schwartz SJ, Vodovotz Y (2003) Isoflavone profile and biological activity of soy bread. J Agric Food Chem 51: 7611–7616.

    Article  CAS  Google Scholar 

  11. Wang H, Murphy PA (1994) Isoflavone Content in Commecial Soybean Foods. J Agric Food Chem 42: 1666–1673.

    Article  CAS  Google Scholar 

  12. Jackson CJC, Dini JP, Lavandier C, Rupasinghe HPV, Faulkner H, Poysa V, Buzzell D, Degrandis S (2002) Effects of processing on the content and composition of isoflavones during manufacturing of soy beverage and tofu. Process Biochem 37: 1117–1123.

    Article  CAS  Google Scholar 

  13. Rickert DA, Meyer MA, Murphy PA (2004) Effect of extraction pH and temperature on isoflavone and saponin partitioning and profile during soy protein isolate production. J Food Sci 69(8): 623–631.

    Article  Google Scholar 

  14. Barbosa ACL, Lajolo FM, Genovese MI (2006) Influence of temperature, pH and ionic strength on the production of isoflavone-rich soy protein isolates. Food Chem 98: 757–766.

    Article  CAS  Google Scholar 

  15. Pinto MS, Lajolo FM, Genovese MI (2005) Effect of storage temperature and water activity on the content and profile of isoflavones, antioxidant activity, and in vitro protein digestibility of soy protein isolates and defatted soy flours. J Agric Food Chem 53: 6340–6346.

    Article  CAS  Google Scholar 

  16. Wang HJ, Murphy PA (1996) Mass balance study of isoflavones during soybean processing. J Agric Food Chem 44: 2377–2383.

    Article  CAS  Google Scholar 

  17. Wang C, Ma Q, Pagadala S, Sherrard MS, Krishnan PG (1998) Changes of isoflavones during processing of soy protein isolates. J Am Chem Soc 75: 337–341.

    Article  CAS  Google Scholar 

  18. Song T, Barua K, Buseman G, Murphy PA (1998) Soy isoflavones analysis: quality control and a new internal standard. Am J Clin Nutr 68: 1474S–1479S.

    CAS  Google Scholar 

  19. Garcia MC, Marina ML, Laborda F, Torre M (1998) Chemical characterization of commercial soybean products. Food Chem 62(3): 325–331.

    Article  Google Scholar 

  20. Tsukamoto C, Shimada S, Igita K, Kudou S, Kokubun M, Okubo K, Kitamura K (1995) Factors affecting isoflavone content in soybean seeds: Changes in isoflavones, saponins, and composition of fatty acids at different temperatures during seed development. J Agric Food Chem 43: 1184–1192.

    Article  CAS  Google Scholar 

  21. Wang C, Sherrard M, Pagadala S, Wixon R, Scott RA (2000) Isoflavone content among maturity group 0 to II soybean. J Am Oil Chem Soc 77(5): 483–487.

    Article  CAS  Google Scholar 

  22. Lee SJ, Yan W, Ahn JK, Chung IIIM (2003) Effects of year, site, genotype and their interactions on various soybean isoflavones. Field Crops Res 81: 181–192.

    Article  Google Scholar 

  23. Umphress ST, Murphy SP, Franke AA, Custer LJ, Blitz CL (2005) Isoflavone content of foods with soy additives. J Food Comp Anal 18: 533–550.

    Article  CAS  Google Scholar 

  24. Eldridge AC, Kwolek WF (1983) Soybean isoflavones: effect of environment and variety on composition. J Agric Food Chem 31: 394–396.

    Article  CAS  Google Scholar 

  25. Carrão-Panizzi MC, Keisuke K, Beléia ADP, Oliveira MCN (1998) Influence of growth locations on isoflavone contents in Brazilian soybean cultivars. Breeding Sci 48: 409–413.

    Google Scholar 

  26. Carrão-Panizzi MC, Beléia ADP, Keisuke K, Oliveira MCN (1999) Effects of genetics and environment on isoflavone content of soybean from different regions of Brazil. Pesq Agropec Bras 34(10): 1787–1795.

    Article  Google Scholar 

  27. Wang H, Murphy PA (1994) Isoflavone composition of American and Japanese soybean in Iowa: effects of variety, crop year, and location. J Agric Food Chem 42: 1674–1677.

    Article  CAS  Google Scholar 

  28. Coward L, Smith M, Kirk M, Barnes S (1998) Chemical modification of isoflavones in soyfoods during cooking and processing. Am J Clin Nutr 68: 1486S–1491S.

    CAS  Google Scholar 

  29. Setchell KDR (1998) Phytoestrogens: the biochemistry, physiology, and implications for human health of soy isoflavones. Am J Clin Nutr 68(suppl): 1333S–1346S.

    CAS  Google Scholar 

  30. Mahungu SM, Diaz-Mercado S, Li J, Schwenk M, Singletary K, Faller J (1999) Stability of isoflavones during extrusion processing of corn/soy mixture. J Agric Food Chem 47: 279–284.

    Article  CAS  Google Scholar 

  31. Izumi T, Piskula MK, Osawa S, Obata A, Tobe K, Saito M, Kataoka S, Kubota Y, Kikuchi M (2000) Soy isoflavone aglycones are absorbed faster and in higher amounts than their glucosides in humans. J Nutr 130: 1695–1699.

    CAS  Google Scholar 

  32. Yeh HY, Su NW, Lee MH (2005) Chemical compositions and physicochemical properties of the fiber-rich materials prepared from shoyu mash residue. J Agric Food Chem 53: 4361–4366.

    Article  CAS  Google Scholar 

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Acknowledgements

To FAPESP and CNPq, for the financial support to this work, CNPQ and CAPES for the grants to Márcia da Silva Pinto and Ana Cristina Lopes Barbosa.

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Authors and Affiliations

  1. Departamento de Alimentos e Nutrição Experimental, Universidade de São Paulo, Av. Prof. Lineu Prestes, 580, Bloco 14, Cep 05508-900, São Paulo, SP, Brazil

    Maria InÉs Genovese, Ana Cristina Lopes Barbosa, MÁrcia Da Silva Pinto & Franco M. Lajolo

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  1. Maria InÉs Genovese
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  2. Ana Cristina Lopes Barbosa
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  3. MÁrcia Da Silva Pinto
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  4. Franco M. Lajolo
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Correspondence to Maria InÉs Genovese.

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Genovese, M.I., Barbosa, A.C.L., Pinto, M.D.S. et al. Commercial Soy Protein Ingredients as Isoflavone Sources for Functional Foods. Plant Foods Hum Nutr 62, 53–58 (2007). https://doi.org/10.1007/s11130-007-0041-0

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