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Effect of Anthocyanin-Rich Extract from Black Rice (Oryza sativa L. indica) on Hyperlipidemia and Insulin Resistance in Fructose-Fed Rats


This study was designed to evaluate the effect of an anthocyanin-rich extract from black rice on hyperlipidemia and insulin resistance in fructose-fed rats. Rats fed fructose diet for 4 weeks exhibited significantly higher plasma insulin levels and lower insulin sensitivity than the control rats fed AIN-93G diet. Dietary supplementation with the anthocyanin-rich extract (5 g/kg of high-fructose diet) prevented the development of fructose-induced insulin resistance. After fructose-induced insulin resistance had been established, 4-week treatment with the anthocyanin-rich extract (5 g/kg of high-fructose diet) or pioglitazone (270 mg/kg of high-fructose diet) ameliorated the glucose intolerance and hyperlipidemia, but the extract failed to reverse the fructose-induced hyperinsulinemia as pioglitazone did. In addition, rats supplemented by the extract exhibited lower oxidative stress than the fructose-fed controls, as indicated by the lower concentrations of plasma thiobarbituric acid reactive substances and blood oxidized glutathione. Overall, these results suggest that the anthocyanin-rich extract from black rice improves certain metabolic abnormalities associated with diets high in fructose.

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Figure 1.



Anthocyanin-rich extract from black rice


Area under the curve


Body weight


Free fatty acid


Reduced glutathione


Oxidized glutathione


High-density lipoprotein cholesterol


High fructose


Relative-value of homeostasis model


Low-density lipoprotein cholesterol


Thiobarbituric acid reactive substances


Total cholesterol




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We particularly thank Prof. Mingwei Zhang in the Key Laboratory of Functional Food, Ministry of Agriculture of the P.R. China, for providing the black rice pigment fraction. This work was supported by the research grant from National Natural Science Foundation of China (#30025-037) and China Medical Board of New York Inc. (CMB, #98-677).

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Correspondence to WENHUA LING.

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GUO, H., LING, W., WANG, Q. et al. Effect of Anthocyanin-Rich Extract from Black Rice (Oryza sativa L. indica) on Hyperlipidemia and Insulin Resistance in Fructose-Fed Rats. Plant Foods Hum Nutr 62, 1–6 (2007).

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Key words:

  • Anthocyanin
  • Black rice
  • Hyperlipidemia
  • Insulin resistance
  • Metabolic syndrome
  • Oxidative stress