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Effects of Two Energy-Restricted Diets Containing Different Fruit Amounts on Body Weight Loss and Macronutrient Oxidation


The consumption of specific foods in energy-restricted diets may affect the weight loss process. The purpose of this research was to evaluate whether obese women following two hypocaloric diets with distinct fruit content differ in weight loss and metabolic responses. Fifteen obese women were included, who were randomly assigned to follow a low or a high-fruit energy-restricted diet for 8 weeks. The main outcome variables were weight and fat losses. Metabolic measurements concerning macronutrient oxidation were also assessed by using 13C labelled fructose and indirect calorimetry. The induced weight loss was similar for both diets (6.9 ± 2% vs. 6.6 ± 2%, p = 0.785). Both experimental diets similarly improved the lipid plasma profile in the participants, but the cholesterol fall was higher in obese subjects receiving the diet containing more fruit. No statistical differences in lipids carbohydrates and 13C labelled fructose utilisation were observed, but protein oxidation was differently affected by the experimental diets. The compensatory effects of the associated fibre/fructose intake may explain the lack of a specific effect of the fruit amount on hypocaloric diets designed to weight loss, although the increased fibre content from enriched fruit diets may be involved in the favourable effects on cholesterol plasma levels.

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body mass index


central nervous system


resting energy expenditure


respiratory quotient


  1. 1.

    Marti A, Moreno-Aliaga MJ, Hebebrand, J Martinez, JA (2004) Genes, lifestyles and obesity. Int J Obes 28: S29–S36.

    Article  Google Scholar 

  2. 2.

    Thompson D, Wolf AM (2001) The medical-care cost burden of obesity. Obes Rev 2: 189–197.

    Article  CAS  Google Scholar 

  3. 3.

    Bray GA (2003) Risks of obesity. Endocrinol Metab Clin North Am 32: 787–804.

    Article  Google Scholar 

  4. 4.

    Sánchez-Villegas A, Madrigal H, Martínez-González MA, Kearney J, Gibney MJ, de Irala J, Martínez JA (2001) Perception of body image as indicator of weight status in the European Union. J Hum Nutr Diet 14: 93–102.

    Google Scholar 

  5. 5.

    Astrup A, Toubro S, Raben A, Skov AR (1997) The role of low-fat diets and fat substitutes in body weight management: What have we learned from clinical studies? J Am Diet Assoc 97: S82–S87.

    Article  CAS  Google Scholar 

  6. 6.

    Zulet MA, Berkenpas ME, Martinez JA (2005) Comparison of dietary approaches to treat obesity based on the different carbohydrate/fat content: Impact on weight loss and lipid profile. Curr Nutr Food Sci 1: 13–21.

    CAS  Article  Google Scholar 

  7. 7.

    Conceicao de Oliveira M, Sichieri R, Sanchez Moura A (2003) Weight loss associated with a daily intake of three apples or three pears among overweight women. Nutrition 19: 253–256.

    Google Scholar 

  8. 8.

    Rolls BJ, Ello-Martin JA, Tohill BC (2004) What can intervention studies tell us about the relationship between fruit and vegetable consumption and weight management? Nutr Rev 62: 287–301.

    Google Scholar 

  9. 9.

    Pelkman CL, Fishell VK, Maddox DH, Pearson TA, Mauger DT, Kris-Etherton PM (2004) Effects of moderate fat (from monounsaturated fat) and low- fat weight-loss diets on the serum lipid profile and obese men and women. Am J Clin Nutr 79: 204–212.

    CAS  Google Scholar 

  10. 10.

    Foster GD, Wyatt HR, Hill JO, McGuckin BG, Brill C, Mohammmed BS, Szapary PO, Rader DJ, Edman JS, Klein S (2003) A randomized trial of a low carbohydrate diet for obesity. N Engl J Med 348: 2082–2090.

    Article  CAS  Google Scholar 

  11. 11.

    Samaha FF, Iqbal N, Seshadri P, Chicano KL, Daily DA, McGrory J, Williams T, Williams M, Gracely EJ, Stern L (2003) A low carbohydrates compared with a low fat diet in severe obesity. N Engl J Med 348: 2074–2081.

    Article  CAS  Google Scholar 

  12. 12.

    Astrup A, Meinert Larsen T, Harper A (2004) Atkins and other low-carbohydrate diets: Hoax or an effective tool for weight loss? Lancet 364: 897–899.

    Article  Google Scholar 

  13. 13.

    Pawlak DB, Kushner JA, Ludwig DS (2004) Effects of dietary glycaemic index on adiposity, glucose homeostasis, and plasma lipids in animals. Lancet 364: 736–737.

    Article  CAS  Google Scholar 

  14. 14.

    Frost GS, Brynes AE, Bovill-Taylor C, Dornhorst A (2004) A prospective randomised trial to determine the efficacy of a low glycaemic index diet given in addition to healthy eating and weight loss advice in patients with coronary heart disease. Eur J Clin Nutr 58: 121–127.

    Article  CAS  Google Scholar 

  15. 15.

    Bray GA, Nielsen SJ, Popkin BM (2004) Consumption of high-fructose corn syrup in beverages may play a role in the epidemic of obesity. Am J Clin Nutr 79: 537–543.

    CAS  Google Scholar 

  16. 16.

    Elliot SS, Keim NL, Stern JS, Teff K, Havel PJ (2002) Fructose, weight gain, and the insulin resistance syndrome. Am J Clin Nutr 76: 911–922.

    Google Scholar 

  17. 17.

    Marlett JA, McBurney MI, Slavin JL, American Dietetic Association (2002) Position of the American Dietetic Association: Health implications of dietary fiber. J Am Diet Assoc 102: 993–1000.

    Article  Google Scholar 

  18. 18.

    Ferrannini E (1988) The theoretical bases of indirect calorimetry: A review. Metabolism 37: 287–301.

    CAS  Google Scholar 

  19. 19.

    Labayen I, Marques-Lopes I, Martínez JA (1997) Métodos de medida del gasto energético. Nutr Clin 6: 7–18.

    Google Scholar 

  20. 20.

    Friedewald WT, Levy RI, Fredrickson DS (1972) Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18: 499–502.

    CAS  Google Scholar 

  21. 21.

    Ghoos Y, Beaufrere B (1998) 13Cprotein breath tests. Gut 43: 23–24.

    Article  Google Scholar 

  22. 22.

    Bantle JP, Raatz SK, Thomas W, Georgopoulos A (2000) Effects of dietary fructose on plasma lipids in healthy subjects. Am J Clin Nutr 72: 1128–1134.

    CAS  Google Scholar 

  23. 23.

    Teff KL, Elliott SS, Tschop M, Kieffer TJ, Rader D, Heiman M, Townsend RR, Keim NL, Dálessio D, Havel PJ (2004) Dietary fructose reduces circulating insulin and leptin, attenuates postpandrial suppression of ghrelin, and increases tryglicerides in women. J Clin Endocrinol Metab 89: 2963–2972.

    CAS  Google Scholar 

  24. 24.

    Mayes PA (1993) Intermediary metabolism of fructose. Am J Clin Nutr 58: 754–765.

    Google Scholar 

  25. 25.

    Fried SK, Rao SP, Sugars, hypertriglyceridemia, and cardiovascular disease (2003) Am J Clin Nutr 78: 873–880.

    Google Scholar 

  26. 26.

    Smith U (1987) Dietary fibre, diabetes and obesity. Int J Obes 11: 27–31.

    Google Scholar 

  27. 27.

    Jenkins DJ, Axelsen M, Kendall CW, Agustin LS, Vuksan V, Smith U (2000) Dietary fibre, lente carbohydrates and the insulin resistant diseases. Br J Nutr 83: S157–S163.

    CAS  Google Scholar 

  28. 28.

    Shulze MB, Manson JE, Ludwig DS, Colddtiz GA, Stampefer MJ, Willett WC, Hu FB (2004) Sugar-sweetened beverages, weight gain, and incidence of type 2 diabetes in young and middle-aged women. JAMA 292: 927–934.

    Google Scholar 

  29. 29.

    Foster-Powell K, Hofl SH, Brand SH, Brand-Miller JC (2002) International table of glycemic index and glycemic load values. Am J Clin Nutr 76: 5–56.

    CAS  Google Scholar 

  30. 30.

    Labayen I, Forga L, Martinez JA (1999) Nutrient oxidation and metabolic rate as affected by meals containing different proportions of carbohydrate and fat, in healthy young women. Eur J Nutr 38: 158–166.

    Article  CAS  Google Scholar 

  31. 31.

    Hill JO, Peters JC, Reed GW, Schlundt DG, Sharp T, Greene HL (1991) Nutrient balance in humans: Effects of diet composition. Am J Clin Nutr 54: 10–17.

    CAS  Google Scholar 

  32. 32.

    Labayen I, Díez N, Parra D, Gonzalez A, Martinez JA (2004a) Basal and postpandrial substrate oxidation rates in obese women receiving two test meals with different protein content. Clin Nutr 23: 571–578.

    CAS  Google Scholar 

  33. 33.

    Astrup A (1999) Macronutrient balances and obesity: The role of diet and physical activity. Public Health Nutr 2: 341–347.

    CAS  Google Scholar 

  34. 34.

    Labayen I, Diez N, Parra MD, González A, Martínez JA (2004b) Time course changes in macronutrient metabolism inducted by a nutritionally balanced low calorie diet in obese women. Int J Food Sci Nutr 55: 27–35.

    Article  CAS  Google Scholar 

  35. 35.

    Tittelbach TJ, Mattes RD, Gretebeck RJ (2000) Post-exercise substrate utilization after a high glucose vs. High fructose meal during negative energy balance in the obese. Obes Res 8: 496–505.

    CAS  Article  Google Scholar 

  36. 36.

    Due A, Toubro S, Skov AR, Astrup A (2004) Effect of normal-fat diets, either medium or high in protein, on body weight in overweight subjects: A randomised 1-year trial. Int J Obes 28: 1283–1290.

    Article  CAS  Google Scholar 

  37. 37.

    McCaffree J (2004) What you should know about calorie restriction. J Am Diet Assoc 104: 1524–1526.

    Article  Google Scholar 

  38. 38.

    Anderson AS (2005) Obesity prevention and management-evidence. J Hum Nutr Diet 18: 1–2.

    Google Scholar 

  39. 39.

    Yao LH, Jioang YM, Shi J, Tomas-Barberan FA, Datta N, Singanusong R, Chen SS (2004) Flavonoids in food and their health benefits. Plant Foods Hum Nutr 59: 113–122.

    Article  CAS  Google Scholar 

  40. 40.

    Contaldo F, Pasanisi F (2005) Obesity epidemics: Simple or simplicistic answers?. Clin Nutr 24: 1–4.

    Google Scholar 

  41. 41.

    McKee LH, Latner TA (2000) Underutilized sources of dietary fiber: A review. Plant Foods Hum Nutr 55: 285–304.

    Article  CAS  Google Scholar 

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Correspondence to J. Alfredo Martínez.

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Rodríguez, M.C., Parra, M.D., Marques-Lopes, I. et al. Effects of Two Energy-Restricted Diets Containing Different Fruit Amounts on Body Weight Loss and Macronutrient Oxidation. Plant Foods Hum Nutr 60, 219–224 (2005).

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Key Words

  • Fruit
  • Energy-restricted diets
  • Fibre
  • Stable isotopes
  • Weight loss