To investigate hypoglycemic substances from pumpkin, protein-bound polysaccharide was isolated by activity-guided isolation from water soluble substances of the fruits. The protein-bound polysaccharide from pumpkin fruits (PBPP) was identified to consist mainly of polysaccharide (approximately 41.21%) and protein (approximately 10.13%) by anthrone test and Lowry-Folin test. Different doses of PBPP were evaluated for hypoglycemic activity and the effect on serum insulin levels in alloxan diabetic rats. The results indicated that PBPP can obviously increase the levels of serum insulin, reduce the blood glucose levels and improve tolerance of glucose. The hypoglycemic effect of big dose PBPP group (1000 mg/kg body weight) excelled that of small dose PBPP group (500 mg/kg body weight) and antidiabetic agent group. The results suggest that the hypoglycemic effect of PBPP depends on the dose and PBPP possesses the possibility of being developed from a new antidiabetic agent.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Zhang F, Sun D, Li M (1995) Preparation of Astraglus polysaccharide and its therapeutic effect on hepatitis. J Chin Pharm Biotechnol 2: 26–28.
Tian G, Feng Y (1995) Evolution of study on plant polysaccharide. J Chin Tradit Med 20: 441–445.
Wang Q, Hong H, Zhao Z, Shen Y, Cheng G (2002) Effect of polysaccharides-2b from Mudan cortex on type 2 diabetes mellitus rats and its mechanism. Chin Pharmacol Bull 18:456–459.
Konno C, Sugiyama K, Kano M (1984) Isolation and hypoglycaemic activity of panaxans A, B, C, D, and E, glycans of Panax ginseng roots. Planta Med 50: 434–436.
Xu Z, Zhou S, Huang L (2000) Protective effect of Coixan on pancreatic islet β cell injury by Alloxan in rats.Chin Pharmacol Bull 16: 639–642.
Jiangsu New Medical College (1985) Dictionary of Chinese Traditional and Herbal Drugs. Shanghai Science and Technology Publishing Company of China.
Zhang Y, Yao H (2002) Study on effect of hypoglycemia of different type pumpkin. J Chin Food Sci 23: 118–120.
Chen Z, Wang X, Jie Y, Huang C, Zhang G (1994) Study on hypoglycemia and hypotension function of pumpkin powder on human. Jiangxi Chin Med 25: 50.
Xiong X, Cao J (2001) Study of extraction and isolation of effective pumpkin polysaccharide component and its reducing glycemia function. China J MAP 18: 662–664.
Szkudelski T (2001) The mechanism of alloxan and streptozotocin action in β cells of the rat pancreas. Physiol Res 50: 536-546.
Zhang W (1987) Biochemistry about complex polysaccharides. Shanghai Science and Technology Publishing Company of China.
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of carbohydrates andrelated substances. Anal Chem 28: 350–356.
Kim SH, Kim HW, Choi EC, Kim BK (1993) Immunological studies on Collybia confluens. J Korean Cancer Assoc 25: 288–298.
Seong-Kug Eo, Young-So Kim, Chong-Kil Lee, Seong-Sun Han (1999) Antiherpetic activities of various protein-bound polysaccharides isolated from Ganoderma lucidum. J Ethnopharmacol 68: 175–181.
Sone Y, Okuda R, Wada N, Kishida E, Misaki A (1985) Structures and antitumor activities of the polysaccharides isolated from fruiting body and the growing culture of mycelium of Ganoderma lucidum. Agric Biol Chem 49: 2541–2653.
Reaven GM (1988) Role of insulin resistance in human disease. Diabetes 37: 1597–1607.
Grandy SF, Buse MG, Crouch RK (1982) Protective role of superoxide dismutase against diabetogenic drugs. J Clin Invest 70: 650–658.
Yadav S, Vats V, Dhunnoo Y, Grover JK (2002) Hypoglycemic and antihyperglycemic activity of Murraya koenigii leaves in diabetic rats. J Ethnopharmacol 82: 111–116.
About this article
Cite this article
Quanhong, L., Caili, F., Yukui, R. et al. Effects of Protein-Bound Polysaccharide Isolated from Pumpkin on Insulin in Diabetic Rats. Plant Foods Hum Nutr 60, 13–16 (2005). https://doi.org/10.1007/s11130-005-2536-x
- Activity-guided isolation
- Hypoglycemic activity
- Protein-bound polysaccharide