Abstract
The objectives of this study were to measure the chlorophyll fluorescence (ChlF) parameters of Barbula indica (Hook.) Spreng and Conocephalum conicum (L.) Dumort subjected to various light intensities (LI) as a reflection of their adaptability to their habitats. The electron transport rate (ETR) of all plants under 500 μmol m–2 s–1 photosynthetic photon flux density (PPFD) was significantly higher than other LI treatments, implying that these plants could be grown under a specific and optimal light intensity adapted to 500 PPFD conditions. As LI increased from 50 to 2,000 PPFD, we observed in all plants increased non-photochemical quenching (NPQ) and photo-inhibitory quenching (qI) and decreased photosystem II efficiency (ΦPSII), potential quantum efficiency of PSII (Fv/Fm), actual PSII efficiency (ΔF/Fm′%), and Fv/Fm%. In addition, energy-dependent quenching (qE), the light protection system (qE + qZ + qT), and qI increased as ΦPSII decreased and photo-inhibition% increased under 1000, 1500, and 2000 PPFD conditions, suggesting that these plants had higher photo-protective ability under high LI treatments to maintain higher photosynthetic system performance. B. indica plants remained photochemically active and maintained higher qE under 300, 500, and 1000 PPFD, whereas C. conicum qZ + qT exhibited higher photo-protection under 500, 1000, and 1500 PPFD conditions. These ChlF indices can be used for predicting photosynthetic responses to light induction in different bryophytes and provide a theoretical basis for ecological monitoring.
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References
Basile A, Sorbo S, Aprile G, Conte B, Cobianchi RC (2008) Comparison of the heavy metal bioaccumulation capacity of an epiphytic moss and an epiphytic lichen. Environ Pollut 151:401–407. https://doi.org/10.1016/j.envpol.2007.07.004
Carriquí M, Roig-Oliver M, Brodribb TJ, Coopman RE, Gill W, Mark K, Niinemets Ü, Perera-Castro AV, Ribas-Carbo M, Sack L, Tosens T, Waite M, Flexas J (2019) Anatomical constraints to nonstomatal diffusion conductance and photosynthesis in lycophytes and bryophytes. New Phytol 222:1256–1270. https://doi.org/10.1111/nph.15675
Chen C, Lin KH, Lin TC, Huang MY, Chen YC, Huang CC, Wang CW (2023) Responses of photosynthesis and chlorophyll fluorescence during light induction in different seedling ages of Mahonia oiwakensis. Bot Stud 64:5. https://doi.org/10.1186/s40529-023-00369-w
Dai Y, Shen Z, Liu Y, Wang L, Hannaway D, Lu H (2009) Effects of shade treatments on the photosynthetic capacity, chlorophyll fluorescence, and chlorophyll content of Tetrastigma hemsleyanum Diels et Gilg. Environ Exp Bot 65(2–3):177–182
D’Ambrosio N, Arena C, De Santo AV (2006) Temperature response of photosynthesis, excitation energy dissipation and alternative electron sinks to carbon assimilation in Beta vulgaris L. Environ Exp Bot 55(3):248–257. https://doi.org/10.1016/j.envexpbot.2004.11.006
Demmig-Adams B, Adams WW III (2006) Photoprotection in an ecological context: the remarkable complexity of thermal energy dissipation. New Phytol 172:11–21. https://doi.org/10.1111/j.1469-8137.2006.01835.x
Demmig-Adams B, Stewart JJ, López-Pozo M, Polutchko SK, Adams WW (2020) Zeaxanthin, a molecule for photo-protection in many different environments. Molecules 25:5825. https://doi.org/10.3390/molecules25245825
Dewir YH, El-Mahrouk MES, Al-Shmgani HS, Rihan HZ, Teixeira da Silva JA, Fuller MP (2015) Photosynthetic and biochemical characterization of in vitro-derived African violet (Saintpaulia ionantha H.Wendl) plants to ex vitro conditions. J Plant Interact 10:101–108. https://doi.org/10.1080/17429145.2015.1018967
Egunyomi A, Oyesiku OO, Bolaji AO (2018) Are the fruiting and non-fruiting acrocarpous moss Barbulaindica (Hooker) Sprengel in Nigeria distinct species? IOSR J Pharm Biol Sci 13:1–5. https://doi.org/10.9790/3008-1301030105
He P, Wright IJ, Zhu S, Onoda Y, Liu H, Li R, Liu X, Hua L, Oyanoghafo OO, Ye Q (2019) Leaf mechanical strength and photosynthetic capacity vary independently across 57 subtropical forest species with contrasting light requirements. New Phytol 223:607–618. https://doi.org/10.1111/nph.15803
La Porta N, Bertamini M, Nedunchezhian N, Raddi P, Muthuchelian K (2005) Photoinhibition of photosynthesis in needles of two cypress (Cupressus sempervirens) clones. Tree Physiol 25:1033–1039. https://doi.org/10.1093/treephys/25.8.1033
Lai YH, Lin KH, Liu CP, Liao TS, Huang MY, Wang CW, Chen CI (2022) Photosynthetic responses of Eulophia dentata, Bletilla formosana, and Saccharum spontaneum under various photosynthetic photon flux density conditions. Photosynthetica 60(4):539–548. https://doi.org/10.32615/ps.2022.050
Leakey ADB, Press MC, Scholes JD (2003) Hightemperature inhibition of photosynthesis is greater under sunflecks than uniform irradiance in a tropical rain forest tree seedling. Plant Cell Environ 26(10):1681–1690. https://doi.org/10.1046/j.1365-3040.2003.01086.x
Levinsh G, Laura G, Didzis E, Ligita L (2020) Relationship between functional traits, functional types, habitat in boreonemoral bryophytes. Proc Latvian Acad Sci 74(3):196–205. https://doi.org/10.2478/prolas-2020-0031
Liepiņa L, Ievinsh G (2013) Potential for fast chlorophyll a fluorescence measurement in bryophyte ecophysiology. Estonian J Ecol 6:137–149. https://doi.org/10.3176/eco.2013.2.05
Lu ZQ, Fan PH, Ji M, Lou HX (2006) Terpenoids and bisbibenzyls from Chinese liverworts Conocephalum conicum and Dumortiera hirsute. J Asian Nat Prod Res 8:187–192. https://doi.org/10.1080/1028602042000325537
Malnoë A (2018) Photoinhibition or photoprotection of photosynthesis? Update on the (newly termed) sustained quenching component qH. Environ Exp Bot 154:123–133. https://doi.org/10.1016/j.envexpbot.2018.05.005
Maresca V, Salbitani G, Moccia F, Cianciullo P, Carraturo F, Sorbo S, Insolvibile M, Carfagna S, Panzella L, Basile A (2022) Antioxidant response to heavy metal pollution of Regi Lagni freshwater in Conocephalum conicum L. (Dum). Ecotoxicol Environ Saf 234:113365. https://doi.org/10.1016/j.ecoenv.2022.113365
Moya I, Loayza H, Lopez ML, Quiroz R, Ounis A, Goulas Y (2019) Canopy chlorophyll fluorescence applied to stress detection using an easy to build microlidar. Photosynth Res 142:1–15. https://doi.org/10.1007/s11120-019-00642-9
Nadal M, Flexas J, Gulias J (2018) Possible link between photosynthesis and leaf modulus of elasticity among vascular plants: a new player in leaf traits relationships? Ecol Lett 21:1372–1379. https://doi.org/10.1111/ele.13103
Orekhova A, Barták M, Casanova-Katny A, Hájek J (2021) Resistance of Antarctic moss Sanionia uncinata to photoinhibition: chlorophyll fluorescence analysis of samples from the western and eastern coasts of the Antarctic Peninsula. Plant Biol 23:653–663. https://doi.org/10.1111/plb.13270
Perera-Castro AV, Nadal M, Flexas J (2020) What drives photosynthesis during desiccation? Mosses and other outliers from the photosynthesis–elasticity trade-off. J Exp Bot 71:6460–6470. https://doi.org/10.1093/jxb/eraa328
Portela FCS, Macieira BPB, Zanetti LV, Gama VN, Silva DM, Milanez CRD, Cuzzuol GRF (2019) How does Cariniana estrellensis respond to different irradiance levels? J For Res 30:31–44. https://doi.org/10.1007/s11676-017-0578-1
Proctor MCF, Bates JW (2018) Chlorophyll-fluorescence measurements in bryophytes: evidence for three main types of light-curve response. J Bryol 40:1–11. https://doi.org/10.1080/03736687.2017.1407280
Proctor MCF, Smirnoff N (2010) Ecophysiology of photosynthesis in bryophytes: major roles for oxygen photoreduction and non-photochemical quenching at high irradiance in mosses with unistratose leaves? Physiol Plant 141:130–140. https://doi.org/10.1111/j.1399-3054.2010.01424.x
Proctor MCF, Smirnoff N (2015) Photoprotection in bryophytes: rate and extent of dark-relaxation of non-photochemical quenching (NPQ) of chlorophyll fluorescence. J Bryol 37:171–177. https://doi.org/10.1179/1743282015Y.0000000001
Son N, Khiem L, Sang N, Tien D, Thang H (2021) Determination of elements due to atmospheric deposition on Barbula indica moss at Dalat, Vietnam using NAA and TXRF techniques. Sains Malaysiana 50(6):1531–1541. https://doi.org/10.17576/jsm-2021-5006-03
Souza CSCR, Santos VAHF, Ferreira MJ, Gonçalves JFC (2017) Biomass, growth and ecophysiological responses of young plants of Bertholletia excelsa Bonpl. subjected to different levels of irradiance. Ciencia Florestal 27(2):557–569
Wang CW, Yang JD, Yao KY, Chen CI, Huang CC, Chu EL, Lai YH (2021) Study on the gas wxchange quantitative and chlorophyll fluorescence of Barbula indica under different irradiations. TW J Biodivers 23(4):38–52
Wang CW, Wong SL, Liao TS, Weng JH, Chen MN, Huang MY, Chen CI (2022) Photosynthesis in response to salinity and submergence in two Rhizophoraceae mangroves adapted to different tidal elevations. Tree Physiol 42:1016–1028. https://doi.org/10.1093/treephys/tpab167
Welc R, Luchowski R, Kluczyk D, Zubik-Duda M, Grudzinski W, Maksim M, Reszczynska E, Sowinski K, Mazur R, Nosalewicz A, Gruszecki WI (2021) Mechanisms shaping the synergism of zeaxanthin and PsbS in photoprotective energy dissipation in the photosynthetic apparatus of plants. Plant J 107:418–433. https://doi.org/10.1111/tpj.15297
Wong SL, Chen CW, Huang HW, Weng JH (2014) Using combined measurements for comparison of light induction of stomatal conductance, electron transport rate and CO2 fixation in woody and fern species adapted to different light regimes. Tree Physiol 32(5):535–544. https://doi.org/10.1093/treephys/tps037
Wu CW, Lin KH, Lee MC, Peng YL, Chou TY, Chang YS (2015) Using chlorophyll fluorescence and vegetation indices to predict the timing of nitrogen demand in Pentas lanceolata. Korean J. Hortic. Sci. Technol. 33, 845–853. https:// doi.org/https://doi.org/10.7235/hort.2015.15043.
Zulfugarov IS, Ham OK, Mishra SR, Kim JY, Nath K, Koo HY, Kim HS, Moon YH, An G, Lee CH (2007) Dependence of reaction center-type energy-dependent quenching on photosystem II antenna size. Biochim Biophys Acta 1767:773–780. https://doi.org/10.1016/j.bbabio.2007.02.021
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This work was partially supported by the Taiwan Government Department for Endemic Species Research Institute.
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CIC, KHL, and CWW conceived and designed the experiments, TCL, MYH, and YCC performed the experiments and analyzed the data, JDY supervised the project, ELC and KYY provided technical assistance to CIC and KHL wrote the article with contributions of all the authors. CWW agrees to serve as the author responsible for contact and ensures communication.
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Chen, CI., Lin, KH., Huang, MY. et al. Photo-protection and photo-inhibition during light induction in Barbula indica and Conocephalum conicum under different light gradients. Photosynth Res 159, 191–202 (2024). https://doi.org/10.1007/s11120-023-01030-0
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DOI: https://doi.org/10.1007/s11120-023-01030-0