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Effects of suppression of chloroplast phosphoglycerate kinase on photosynthesis in rice

Abstract

As chloroplast phosphoglycerate kinase (cpPGK) is one of the enzymes which has the highest capacity among the Calvin–Benson cycle enzymes, it has not been regarded as a determinant for photosynthetic capacity. However, it was reported that the rate of CO2 assimilation decreased under high irradiance and normal [CO2] levels in the Arabidopsis cpPGK-knockdown mutant, implying that cpPGK has a control over photosynthetic capacity at a normal [CO2] level. In the present study, the contribution of cpPGK to photosynthetic capacity was evaluated in transgenic rice plants with decreased amounts of cpPGK protein under high irradiance and various [CO2] levels. The gene encoding cpPGK was suppressed using RNA interference techniques. Two lines of transgenic plants, Pi3 and Pi5, in which the amount of cpPGK protein decreased to 21% and 76% of that in wild-type plants, respectively, were obtained. However, there was no substantial difference in the rates of CO2 assimilation between wild-type and transgenic plants. The rates of CO2 assimilation decreased only slightly at elevated [CO2] levels in the transgenic line Pi3 and did not differ between wild-type plants and the transgenic line Pi5, irrespective of [CO2] level. These results clearly indicate that cpPGK does not have a strong control over photosynthetic capacity at various [CO2] levels in rice.

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All the data that support the findings are included in this MS.

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Acknowledgements

This work was supported by Core Research for Evolutional Science and Technology from the Japan Science and Technology Agency [JPMJCR15O3 to Y.S. and C.M.] and Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science [18H02111 and 21H02084 to Y.S., 16H06379 to A.M.]. We would like to thank Dr. Jochen Kumlehn [Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Germany] for their kind permission to use the pIPKb vector. We would also like to thank the Biotechnology Center in the Faculty of Bioresource Sciences, Akita Prefectural University (Japan) for their aid in the generation of transgenic plants, Dr. Uzuki Matsushima (Iwate University) for assistance with the measurements of photosynthesis, Editage for English editing, and Dr. Louis Irving (Tsukuba University) for his valuable comments.

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YS conceived and designed the experiments. YS, YK, and YT-T generated the transgenic plants. YS, YK, and YT-T performed the experiments. All the authors analyzed and discussed the data. YS wrote the manuscript. All authors reviewed and edited the manuscript.

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Correspondence to Yuji Suzuki.

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Suzuki, Y., Konno, Y., Takegahara-Tamakawa, Y. et al. Effects of suppression of chloroplast phosphoglycerate kinase on photosynthesis in rice. Photosynth Res (2022). https://doi.org/10.1007/s11120-022-00923-w

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  • DOI: https://doi.org/10.1007/s11120-022-00923-w

Keywords

  • Chloroplast phosphoglycerate kinase
  • RNA interference
  • CO2 assimilation
  • Rice (Oryza sativa L.)