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Red-shifted light-harvesting system of freshwater eukaryotic alga Trachydiscus minutus (Eustigmatophyta, Stramenopila)

  • Radek Litvín
  • David BínaEmail author
  • Miroslava Herbstová
  • Marek Pazderník
  • Eva Kotabová
  • Zdenko Gardian
  • Martin Trtílek
  • Ondřej Prášil
  • František Vácha
Original article
  • 182 Downloads

Abstract

Survival of phototrophic organisms depends on their ability to collect and convert enough light energy to support their metabolism. Phototrophs can extend their absorption cross section by using diverse pigments and by tuning the properties of these pigments via pigment–pigment and pigment–protein interaction. It is well known that some cyanobacteria can grow in heavily shaded habitats by utilizing far-red light harvested with far-red-absorbing chlorophylls d and f. We describe a red-shifted light-harvesting system based on chlorophyll a from a freshwater eustigmatophyte alga Trachydiscus minutus (Eustigmatophyceae, Goniochloridales). A comprehensive characterization of the photosynthetic apparatus of T. minutus is presented. We show that thylakoid membranes of T. minutus contain light-harvesting complexes of several sizes differing in the relative amount of far-red chlorophyll a forms absorbing around 700 nm. The pigment arrangement of the major red-shifted light-harvesting complex is similar to that of the red-shifted antenna of a marine alveolate alga Chromera velia. Evolutionary aspects of the algal far-red light-harvesting complexes are discussed. The presence of these antennas in eustigmatophyte algae opens up new ways to modify organisms of this promising group for effective use of far-red light in mass cultures.

Keywords

Light-harvesting protein Violaxanthin Eustigmatophyta Red-shifted LHC Oligomeric LHC Chromatic acclimation 

Notes

Acknowledgements

This research was supported by the Czech Science Foundation under the Grant Numbers 19-28323X (Radek Litvín, David Bína) and GA15-22000S (Martin Trtílek), by institutional support RVO:60077344, Project LO1416 Algatech plus of the programme NPU I (Marek Pazderník, Eva Kotabová, Ondřej Prášil), and European Regional Development Fund (No. CZ.02.1.01/0.0/0.0/15_003/0000441, Zdenko Gardian). Skilled technical assistance of Ivana Hunalová and František Matoušek is gratefully acknowledged.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11120_2019_662_MOESM1_ESM.doc (582 kb)
Supplementary material 1 (DOC 583 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Radek Litvín
    • 1
    • 2
  • David Bína
    • 1
    • 2
    Email author
  • Miroslava Herbstová
    • 1
    • 2
  • Marek Pazderník
    • 1
    • 3
  • Eva Kotabová
    • 1
    • 3
  • Zdenko Gardian
    • 1
    • 2
  • Martin Trtílek
    • 4
  • Ondřej Prášil
    • 1
    • 3
  • František Vácha
    • 1
    • 2
  1. 1.Faculty of ScienceUniversity of South BohemiaČeské BudějoviceCzech Republic
  2. 2.Biology CentreThe Czech Academy of SciencesČeské BudějoviceCzech Republic
  3. 3.Institute of MicrobiologyThe Czech Academy of SciencesTřeboňCzech Republic
  4. 4.PSI (Photon Systems Instruments)DrásovCzech Republic

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