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Effect of sucrose-bound polynuclear iron oxyhydroxide nanoparticles on the efficiency of electron transport in the photosystem II membranes

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Abstract

Effect of water-soluble and stable sucrose-bound iron oxyhydroxide nanoparticles [Fe[III] sucrose complex (FSC)] on the efficiency of electron transport in the photosystem II membranes was studied. FSC significantly increases (by a factor 1.5) the rate of light-induced oxygen evolution in the presence of alternative electron acceptor 2,6-dichloro-p-benzoquinone (DCBQ). Without DCBQ, FSC only slightly (5%) provides the oxygen evolution. Electron transport supported by pair DCBQ + FSC is inhibited by diuron. Maximum of stimulating effect was recorded at Fe(III) concentration 5 µM. In the case of another benzoquinone electron acceptor (2-phenyl-p-benzoquinone and 2,3-dimethyl-p-benzoquinone) and 2,6-dichlorophenolindophenol, stimulating effect of FSC was not observed. Incubation of PSII membranes at different concentrations with FSC is accompanied by binding of Fe(III) by membrane components but only about 50% of iron can be extracted by membranes from Fe(III) solution at pH 6.5. This result implies the heterogeneity of FSC solution in a buffer. The heterogeneity depends on pH and decreases with its rising. At pH around 9.0 Fe(III), sucrose solution is homogeneous. The study of pH effect has shown that stimulation of electron transport is induced only by iron cations which can be bound by membranes. Not extractable iron pool cannot activate electron transfer from oxygen-evolving complex to DCBQ.

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Abbreviations

BPDS:

Bathophenanthroline disulfonic acid

Chl:

Chlorophyll

DCMU:

3-(3,4-Dichlorophenyl)-1,1-dimethylurea

DCBQ:

2,6-Dichloro-p-benzoquinone

DCPIP:

2,6-Dichlorophenolindophenol

DMBQ:

2,3-Dimethyl-p-benzoquinone

HEPES:

N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid

FSC:

Fe(III) sucrose complex

MES:

2-(N-morpholino)-ethanesulfonic acid

OEC:

Oxygen-evolving complex

PQ:

Plastoquinone

PPBQ:

2-Phenyl-p-benzoquinone

PSII:

Photosystem II

PSII(-Ca):

Ca2+-depleted PSII membranes

RC:

Reaction center

Tris:

Tris(hydroxymethyl)aminomethane

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Acknowledgements

We are very grateful to Dr. Kholina E.G. for helping in the measurement of zeta potential.

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  1. Department of Biophysics, Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia, 119234

    B. К. Semin, L. N. Davletshina & A. B. Rubin

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  1. B. К. Semin
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  2. L. N. Davletshina
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  3. A. B. Rubin
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Correspondence to B. К. Semin.

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Semin, B.К., Davletshina, L.N. & Rubin, A.B. Effect of sucrose-bound polynuclear iron oxyhydroxide nanoparticles on the efficiency of electron transport in the photosystem II membranes. Photosynth Res 142, 57–67 (2019). https://doi.org/10.1007/s11120-019-00647-4

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