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Exogenous putrescine alleviates photoinhibition caused by salt stress through cooperation with cyclic electron flow in cucumber

  • Xinyi Wu
  • Sheng Shu
  • Yu Wang
  • Ruonan Yuan
  • Shirong GuoEmail author
Original Article
  • 131 Downloads

Abstract

When plants suffer from abiotic stresses, cyclic electron flow (CEF) is induced for photo-protection. Putrescine (Put), a primary polyamine in chloroplasts, plays a critical role in stress tolerance. However, the relationship between CEF and Put in chloroplasts for photo-protection is unknown. In this study, we investigated the role of Put-induced CEF for salt tolerance in cucumber plants (Cucumis sativus L). Treatment with 90 mM NaCl and/or Put did not influence the maximum photochemical efficiency of PSII (Fv/Fm), but the photoactivity of PSI was severely inhibited by NaCl. Salt stress induced a high level of CEF; moreover, plants treated with both NaCl and Put exhibited much higher CEF activity and ATP accumulation than those exhibited by single-salt-treated plants to provide an adequate ATP/NADPH ratio for plant growth. Furthermore, Put decreased the trans-membrane proton gradient (ΔpH), which was accompanied by reduced pH-dependent non-photochemical quenching (NPQ) and an increased the effective quantum yield of PSII (Y(II)). The ratio of NADP+/NADPH increased significantly with Put in salt-stressed leaves compared with the ratio in leaves treated with NaCl, indicating that Put relieved over-reduction pressure at the PSI acceptor side caused by salt stress. Collectively, our results suggest that exogenous Put creates an excellent condition for CEF promotion: a large amount of pmf is predominantly stored as Δψ, resulting in moderate lumen pH and low NPQ, while maintaining high rates of ATP synthesis (high pmf).

Keywords

Cyclic electron flow Cucumber Photoprotection Putrescine Salt stress 

Abbreviations

Put

Putrescine

CEF

Cyclic electron flow

NPQ

Non-photochemical quenching

PGR5

Protein gradient regulation 5

PGRL1

PGR-like 1

NDH

NAD(P)H dehydrogenase

LEF

Linear electron flow

PAs

Polyamines

Notes

Acknowledgements

Thanks to my supervisor, Guo Shirong, for his valuable support for my work. I am grateful to the numerous individuals who participated in this research. Mr. Sheng Shu and Mr. Yu Wang provided critical discussion and comments, I also thank Ruonan Yuan for help with the experiment technology.

Funding

This work was supported by the National Natural Science Foundation of China (Nos. 31672199 and 31471869) and was supported by China Agriculture Research System (CARS-23-B12).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11120_2019_631_MOESM1_ESM.docx (1.8 mb)
Supplementary material 1 (DOCX 1878 KB)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.College of HorticultureNanjing Agricultural UniversityNanjingChina
  2. 2.Nanjing Agricultural University (Suqian) Academy of Protected HorticultureSuqianChina

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