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Photosynthesis Research

, Volume 137, Issue 2, pp 263–280 | Cite as

The intracellular distribution of inorganic carbon fixing enzymes does not support the presence of a C4 pathway in the diatom Phaeodactylum tricornutum

  • Daniela Ewe
  • Masaaki Tachibana
  • Sae Kikutani
  • Ansgar Gruber
  • Carolina Río Bártulos
  • Grzegorz Konert
  • Aaron Kaplan
  • Yusuke Matsuda
  • Peter G. Kroth
Original Article

Abstract

Diatoms are unicellular algae and important primary producers. The process of carbon fixation in diatoms is very efficient even though the availability of dissolved CO2 in sea water is very low. The operation of a carbon concentrating mechanism (CCM) also makes the more abundant bicarbonate accessible for photosynthetic carbon fixation. Diatoms possess carbonic anhydrases as well as metabolic enzymes potentially involved in C4 pathways; however, the question as to whether a C4 pathway plays a general role in diatoms is not yet solved. While genome analyses indicate that the diatom Phaeodactylum tricornutum possesses all the enzymes required to operate a C4 pathway, silencing of the pyruvate orthophosphate dikinase (PPDK) in a genetically transformed cell line does not lead to reduced photosynthetic carbon fixation. In this study, we have determined the intracellular location of all enzymes potentially involved in C4-like carbon fixing pathways in P. tricornutum by expression of the respective proteins fused to green fluorescent protein (GFP), followed by fluorescence microscopy. Furthermore, we compared the results to known pathways and locations of enzymes in higher plants performing C3 or C4 photosynthesis. This approach revealed that the intracellular distribution of the investigated enzymes is quite different from the one observed in higher plants. In particular, the apparent lack of a plastidic decarboxylase in P. tricornutum indicates that this diatom does not perform a C4-like CCM.

Keywords

C4 photosynthesis Chloroplast Green fluorescent protein (GFP) Carboxylation Decarboxylation 

Notes

Acknowledgements

We are grateful to D. Ballert (Universität Konstanz) for the genetic transformation of P. tricornutum and the cultivation of the transformed cell lines; to the Bioimaging Center at the University of Konstanz for providing the LSM 510 META; to Dr. Katsura Izui for providing the DCDP and to Dr. Pavel Hrouzek (Centre Algatech) for providing the MitoTracker. This study was supported by the German-Israel Foundation for Scientific Research and Development (GIF; Project No. G-1011-199.12/2008 to A.K. and P.G.K.); the University of Konstanz, the Grant-in-Aid for Scientific Research B (Grant No. 24310015 to Y. M.); the Grant-in-Aid for Scientific Research on Innovative Areas (Grant No. 16H06557 to Y. M.); the Japan Society for the Promotion of Science (JSPS; Grant No. 26870750 to S. K.) and by a 2 years Postdoctoral Researcher Fellowship (Mzdová podpora postdoktorandů) from the Academy of Sciences of the Czech Republic (to D.E.).

Supplementary material

11120_2018_500_MOESM1_ESM.tif (36 mb)
Online Resource Figure 5Additional images of proteins which subcellular localization has been experimentally proven in a second and independent laboratory. Presented are fluorescence microscopy images showing transformed P. tricornutum cells with expressed protein::GFP fusion proteins of PEPCK, PPDK and ME1. Bright field, chlorophyll auto-fluorescence (red), GFP fluorescence (green) and a merged image, showing an overlap of the chlorophyll auto- and GFP fluorescence, are shown from left to right. Scale bars of each image represent 5 µm. “full” or “pre” suffixes after the enzyme’s name indicate whether a full length or pre-sequence was used for plasmid construction. PEPCK and ME1 are located in the mitochondrion and PPDK is located in the cytosol. P. tricornutum strain UTEX 642 was used to generate the PPDKfull and strain UTEX 646 was used to generate the PEPCKpre and ME1pre transformed cell lines. Abbreviations: PEPCK: phosphoenolpyruvate carboxykinase; PPDK: pyruvate phosphate dikinase; ME: malic enzyme. (TIF 37800 KB)
11120_2018_500_MOESM2_ESM.docx (17 kb)
Online Resource Table IV (DOCX 17 KB)
11120_2018_500_MOESM3_ESM.xlsx (38 kb)
Online Resource Table V—A list of enzymes including protein IDs and localizations that are mentioned in this study. “Target P” (Emanuelsson et al. 2000) shows the highest probability for the predicted subcellular localization. The chosen organism groups are “Non-plant” for P. tricornutum and “Plant” for A. thaliana. The output style is: cTP - chloroplast transit peptide; mTP - mitochondrial transit peptide; SP - secretory pathway, other - cytosol, “cleavage site” indicates the sequence motif surrounding the signal peptide cleavage site predicted by SignalP 3.0 NN (Bendtsen et al. 2004) (*indicates manual predictions of the putative cleavage site motif), “localization experiment” indicates the results of experimental localizations cited in “reference/comments”. (XLSX 37 KB)

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Fachbereich BiologieUniversität KonstanzKonstanzGermany
  2. 2.Department of Bioscience, School of Science and TechnologyKwansei Gakuin UniversitySandaJapan
  3. 3.Department of Plant and Environmental Sciences, Edmond J. Safra Campus–Givat RamHebrew University of JerusalemJerusalemIsrael
  4. 4.Centre Algatech, Institute of Microbiology of the Czech Academy of SciencesTřeboňCzech Republic
  5. 5.Lion Corporation Pharmaceutical Laboratories No.1OdawaraJapan
  6. 6.Tech Manage Corp.TokyoJapan
  7. 7.Biology Centre, Institute of ParasitologyCzech Academy of SciencesČeské BudějoviceCzech Republic

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